Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0483 and Psest_0484 overlap by 4 nucleotides Psest_0484 and Psest_0485 overlap by 4 nucleotides Psest_0485 and Psest_0486 overlap by 4 nucleotides
Psest_0483: Psest_0483 - DNA topoisomerase IV, B subunit, proteobacterial, at 532,502 to 534,481
_0483
Psest_0484: Psest_0484 - hypothetical protein, at 534,478 to 535,461
_0484
Psest_0485: Psest_0485 - clan AA aspartic protease, TIGR02281 family, at 535,458 to 535,985
_0485
Psest_0486: Psest_0486 - DNA topoisomerase IV, A subunit, proteobacterial, at 535,982 to 538,234
_0486
Position (kb) 535
536 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 534.675 kb on + strand, within Psest_0484 at 534.759 kb on + strand, within Psest_0484 at 534.759 kb on + strand, within Psest_0484 at 534.841 kb on + strand, within Psest_0484 at 535.094 kb on + strand, within Psest_0484 at 535.095 kb on - strand, within Psest_0484 at 535.315 kb on + strand, within Psest_0484 at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.612 kb on + strand, within Psest_0485 at 535.618 kb on + strand, within Psest_0485 at 535.618 kb on + strand, within Psest_0485 at 535.619 kb on - strand, within Psest_0485 at 535.619 kb on - strand, within Psest_0485 at 535.619 kb on - strand, within Psest_0485 at 535.619 kb on - strand, within Psest_0485 at 535.664 kb on + strand, within Psest_0485 at 535.665 kb on - strand, within Psest_0485
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 534,675 + Psest_0484 0.20 +0.2 534,759 + Psest_0484 0.29 +0.6 534,759 + Psest_0484 0.29 +1.1 534,841 + Psest_0484 0.37 +0.9 535,094 + Psest_0484 0.63 +3.0 535,095 - Psest_0484 0.63 -1.0 535,315 + Psest_0484 0.85 +0.5 535,371 + -1.4 535,371 + -0.8 535,371 + -1.2 535,371 + -1.3 535,371 + +0.3 535,612 + Psest_0485 0.29 -1.1 535,618 + Psest_0485 0.30 -1.3 535,618 + Psest_0485 0.30 -0.8 535,619 - Psest_0485 0.30 -1.3 535,619 - Psest_0485 0.30 +0.6 535,619 - Psest_0485 0.30 -2.3 535,619 - Psest_0485 0.30 -2.4 535,664 + Psest_0485 0.39 +0.8 535,665 - Psest_0485 0.39 -0.1
Or see this region's nucleotide sequence