Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0146 and Psest_0147 are separated by 191 nucleotides Psest_0147 and Psest_0148 are separated by 88 nucleotides Psest_0148 and Psest_0149 are separated by 11 nucleotides Psest_0149 and Psest_0150 are separated by 26 nucleotides
Psest_0146: Psest_0146 - Uncharacterized protein involved in outer membrane biogenesis, at 144,376 to 146,601
_0146
Psest_0147: Psest_0147 - Acetyltransferase, GNAT family., at 146,793 to 147,191
_0147
Psest_0148: Psest_0148 - nitrate transport ATP-binding subunits C and D, at 147,280 to 148,062
_0148
Psest_0149: Psest_0149 - nitrate ABC transporter, permease protein, at 148,074 to 148,943
_0149
Psest_0150: Psest_0150 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 148,970 to 150,301
_0150
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.499 kb on - strand at 146.499 kb on - strand at 146.499 kb on - strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.616 kb on - strand at 146.616 kb on - strand at 146.616 kb on - strand at 146.642 kb on + strand at 146.643 kb on - strand at 146.643 kb on - strand at 146.644 kb on + strand at 146.644 kb on + strand at 146.644 kb on + strand at 146.795 kb on - strand at 147.087 kb on - strand, within Psest_0147 at 147.091 kb on + strand, within Psest_0147 at 147.091 kb on + strand, within Psest_0147 at 147.092 kb on - strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.145 kb on - strand, within Psest_0147 at 147.181 kb on + strand at 147.181 kb on + strand at 147.181 kb on + strand at 147.182 kb on - strand at 147.231 kb on + strand at 147.338 kb on + strand at 147.338 kb on + strand at 147.339 kb on - strand at 147.339 kb on - strand at 147.339 kb on - strand at 147.662 kb on + strand, within Psest_0148 at 147.662 kb on + strand, within Psest_0148 at 147.663 kb on - strand, within Psest_0148 at 147.783 kb on - strand, within Psest_0148 at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.157 kb on - strand at 148.291 kb on - strand, within Psest_0149 at 148.306 kb on + strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.342 kb on + strand, within Psest_0149 at 148.627 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.700 kb on - strand, within Psest_0149 at 148.700 kb on - strand, within Psest_0149 at 149.004 kb on + strand at 149.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 146,498 + -0.1 146,498 + -0.2 146,498 + -0.2 146,498 + +0.3 146,498 + -1.5 146,498 + -2.2 146,498 + -1.3 146,499 - +0.4 146,499 - -0.2 146,499 - +0.0 146,615 + -1.7 146,615 + +0.6 146,615 + +0.4 146,615 + -1.3 146,615 + +1.4 146,615 + +0.4 146,615 + -0.1 146,615 + -0.5 146,615 + -0.5 146,616 - -0.4 146,616 - -1.8 146,616 - +1.0 146,642 + +0.6 146,643 - +0.2 146,643 - +0.8 146,644 + -1.0 146,644 + -2.7 146,644 + -2.5 146,795 - +0.7 147,087 - Psest_0147 0.74 +0.5 147,091 + Psest_0147 0.75 +0.1 147,091 + Psest_0147 0.75 -2.9 147,092 - Psest_0147 0.75 +0.2 147,124 + Psest_0147 0.83 +1.9 147,124 + Psest_0147 0.83 -0.9 147,124 + Psest_0147 0.83 +0.8 147,125 - Psest_0147 0.83 -0.5 147,125 - Psest_0147 0.83 +0.0 147,125 - Psest_0147 0.83 +1.9 147,125 - Psest_0147 0.83 +0.5 147,125 - Psest_0147 0.83 -0.4 147,125 - Psest_0147 0.83 +0.5 147,145 - Psest_0147 0.88 +0.8 147,181 + -2.3 147,181 + -0.4 147,181 + -0.9 147,182 - +0.9 147,231 + +0.1 147,338 + +0.3 147,338 + -1.0 147,339 - +0.4 147,339 - +0.3 147,339 - -1.0 147,662 + Psest_0148 0.49 -0.9 147,662 + Psest_0148 0.49 -1.0 147,663 - Psest_0148 0.49 -0.5 147,783 - Psest_0148 0.64 -0.8 148,055 + -1.9 148,055 + +0.7 148,055 + +1.1 148,055 + +0.7 148,055 + +0.2 148,055 + +0.6 148,055 + -0.3 148,055 + -0.7 148,056 - +1.1 148,056 - +0.5 148,056 - +0.0 148,056 - +0.3 148,056 - +0.7 148,056 - -2.1 148,056 - +0.2 148,056 - +0.6 148,056 - +0.6 148,156 + +0.2 148,156 + -1.0 148,156 + +0.2 148,156 + -0.1 148,157 - -0.7 148,291 - Psest_0149 0.25 -2.4 148,306 + Psest_0149 0.27 -0.7 148,307 - Psest_0149 0.27 +0.1 148,307 - Psest_0149 0.27 +0.3 148,307 - Psest_0149 0.27 -0.3 148,307 - Psest_0149 0.27 -0.3 148,307 - Psest_0149 0.27 +0.8 148,307 - Psest_0149 0.27 +2.8 148,307 - Psest_0149 0.27 -0.3 148,307 - Psest_0149 0.27 +0.6 148,307 - Psest_0149 0.27 +0.3 148,342 + Psest_0149 0.31 -0.9 148,627 + Psest_0149 0.64 +0.1 148,699 + Psest_0149 0.72 +1.4 148,699 + Psest_0149 0.72 -0.9 148,699 + Psest_0149 0.72 -1.1 148,700 - Psest_0149 0.72 +0.5 148,700 - Psest_0149 0.72 +0.0 149,004 + +0.4 149,005 - +1.2
Or see this region's nucleotide sequence