Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0279

Experiment: L-Valine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0277 and Psest_0278 overlap by 4 nucleotidesPsest_0278 and Psest_0279 are separated by 26 nucleotidesPsest_0279 and Psest_0280 are separated by 9 nucleotides Psest_0277: Psest_0277 - non-canonical purine NTP pyrophosphatase, rdgB/HAM1 family, at 292,995 to 293,588 _0277 Psest_0278: Psest_0278 - hypothetical protein, at 293,585 to 294,007 _0278 Psest_0279: Psest_0279 - methionine biosynthesis protein MetW, at 294,034 to 294,633 _0279 Psest_0280: Psest_0280 - homoserine O-acetyltransferase, at 294,643 to 295,782 _0280 Position (kb) 294 295Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 293.332 kb on + strand, within Psest_0277at 293.332 kb on + strand, within Psest_0277at 293.333 kb on - strand, within Psest_0277at 293.649 kb on - strand, within Psest_0278at 293.703 kb on + strand, within Psest_0278at 293.703 kb on + strand, within Psest_0278at 293.704 kb on - strand, within Psest_0278at 293.704 kb on - strand, within Psest_0278at 293.704 kb on - strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.913 kb on + strand, within Psest_0278at 293.913 kb on + strand, within Psest_0278at 293.913 kb on + strand, within Psest_0278at 293.913 kb on + strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.925 kb on + strand, within Psest_0278at 293.926 kb on - strand, within Psest_0278at 293.926 kb on - strand, within Psest_0278at 293.926 kb on - strand, within Psest_0278at 294.007 kb on + strandat 294.007 kb on + strandat 294.007 kb on + strandat 294.008 kb on - strandat 294.008 kb on - strandat 294.013 kb on + strandat 294.059 kb on + strandat 294.060 kb on - strandat 294.218 kb on + strand, within Psest_0279at 294.218 kb on + strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.227 kb on + strand, within Psest_0279at 294.227 kb on + strand, within Psest_0279at 294.228 kb on - strand, within Psest_0279at 294.228 kb on - strand, within Psest_0279at 294.644 kb on + strandat 294.644 kb on + strandat 294.645 kb on - strandat 294.646 kb on + strandat 294.646 kb on + strandat 294.646 kb on + strandat 294.646 kb on + strandat 294.646 kb on + strandat 294.646 kb on + strandat 294.647 kb on - strandat 294.647 kb on - strandat 294.665 kb on + strandat 294.743 kb on + strandat 294.744 kb on - strandat 294.905 kb on + strand, within Psest_0280at 294.906 kb on - strand, within Psest_0280at 294.906 kb on - strand, within Psest_0280at 294.906 kb on - strand, within Psest_0280at 294.932 kb on + strand, within Psest_0280at 294.933 kb on - strand, within Psest_0280at 294.933 kb on - strand, within Psest_0280at 294.933 kb on - strand, within Psest_0280at 294.933 kb on - strand, within Psest_0280at 294.933 kb on - strand, within Psest_0280at 294.933 kb on - strand, within Psest_0280at 294.937 kb on - strand, within Psest_0280at 294.937 kb on - strand, within Psest_0280at 294.937 kb on - strand, within Psest_0280at 294.983 kb on + strand, within Psest_0280at 294.984 kb on - strand, within Psest_0280at 294.984 kb on - strand, within Psest_0280at 295.019 kb on + strand, within Psest_0280at 295.019 kb on + strand, within Psest_0280at 295.019 kb on + strand, within Psest_0280at 295.019 kb on + strand, within Psest_0280at 295.020 kb on - strand, within Psest_0280at 295.082 kb on + strand, within Psest_0280at 295.083 kb on - strand, within Psest_0280at 295.083 kb on - strand, within Psest_0280at 295.419 kb on - strand, within Psest_0280at 295.512 kb on + strand, within Psest_0280at 295.512 kb on + strand, within Psest_0280at 295.512 kb on + strand, within Psest_0280at 295.513 kb on - strand, within Psest_0280at 295.513 kb on - strand, within Psest_0280at 295.513 kb on - strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.516 kb on + strand, within Psest_0280at 295.517 kb on - strand, within Psest_0280at 295.517 kb on - strand, within Psest_0280at 295.517 kb on - strand, within Psest_0280at 295.517 kb on - strand, within Psest_0280at 295.517 kb on - strand, within Psest_0280at 295.560 kb on + strand, within Psest_0280

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (N)
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293,332 + Psest_0277 0.57 +1.3
293,332 + Psest_0277 0.57 -0.3
293,333 - Psest_0277 0.57 +0.9
293,649 - Psest_0278 0.15 +0.8
293,703 + Psest_0278 0.28 -1.8
293,703 + Psest_0278 0.28 -0.1
293,704 - Psest_0278 0.28 +1.0
293,704 - Psest_0278 0.28 +0.1
293,704 - Psest_0278 0.28 +1.0
293,706 + Psest_0278 0.29 +2.1
293,706 + Psest_0278 0.29 -2.1
293,706 + Psest_0278 0.29 -1.3
293,706 + Psest_0278 0.29 +0.4
293,706 + Psest_0278 0.29 -0.5
293,707 - Psest_0278 0.29 -2.8
293,707 - Psest_0278 0.29 +0.8
293,707 - Psest_0278 0.29 +1.6
293,707 - Psest_0278 0.29 +0.4
293,707 - Psest_0278 0.29 -0.0
293,913 + Psest_0278 0.78 +0.5
293,913 + Psest_0278 0.78 +1.0
293,913 + Psest_0278 0.78 +0.6
293,913 + Psest_0278 0.78 +0.2
293,914 - Psest_0278 0.78 +0.0
293,914 - Psest_0278 0.78 -0.1
293,914 - Psest_0278 0.78 -0.3
293,914 - Psest_0278 0.78 -1.1
293,914 - Psest_0278 0.78 +0.7
293,914 - Psest_0278 0.78 -0.1
293,914 - Psest_0278 0.78 +1.6
293,914 - Psest_0278 0.78 -0.1
293,925 + Psest_0278 0.80 +0.8
293,926 - Psest_0278 0.81 +1.8
293,926 - Psest_0278 0.81 +1.0
293,926 - Psest_0278 0.81 -0.3
294,007 + +0.2
294,007 + -0.4
294,007 + +0.4
294,008 - +0.3
294,008 - -0.1
294,013 + -0.4
294,059 + -2.2
294,060 - -0.9
294,218 + Psest_0279 0.31 -0.9
294,218 + Psest_0279 0.31 -0.8
294,219 - Psest_0279 0.31 -4.1
294,219 - Psest_0279 0.31 -3.3
294,219 - Psest_0279 0.31 -1.9
294,219 - Psest_0279 0.31 -2.5
294,219 - Psest_0279 0.31 -3.3
294,219 - Psest_0279 0.31 -3.5
294,219 - Psest_0279 0.31 -3.0
294,227 + Psest_0279 0.32 -2.2
294,227 + Psest_0279 0.32 -2.2
294,228 - Psest_0279 0.32 -4.5
294,228 - Psest_0279 0.32 -2.2
294,644 + +0.8
294,644 + -0.9
294,645 - -0.9
294,646 + -0.6
294,646 + +0.3
294,646 + -2.0
294,646 + +0.3
294,646 + -0.4
294,646 + -2.0
294,647 - -3.3
294,647 - -0.6
294,665 + -4.2
294,743 + -1.9
294,744 - -4.9
294,905 + Psest_0280 0.23 -1.8
294,906 - Psest_0280 0.23 -2.3
294,906 - Psest_0280 0.23 -2.8
294,906 - Psest_0280 0.23 -2.1
294,932 + Psest_0280 0.25 -2.5
294,933 - Psest_0280 0.25 +0.3
294,933 - Psest_0280 0.25 -2.4
294,933 - Psest_0280 0.25 +0.9
294,933 - Psest_0280 0.25 -2.9
294,933 - Psest_0280 0.25 -3.5
294,933 - Psest_0280 0.25 -2.9
294,937 - Psest_0280 0.26 -3.3
294,937 - Psest_0280 0.26 -2.8
294,937 - Psest_0280 0.26 -0.8
294,983 + Psest_0280 0.30 -1.9
294,984 - Psest_0280 0.30 -1.4
294,984 - Psest_0280 0.30 -3.7
295,019 + Psest_0280 0.33 -4.0
295,019 + Psest_0280 0.33 -1.4
295,019 + Psest_0280 0.33 -3.1
295,019 + Psest_0280 0.33 -2.0
295,020 - Psest_0280 0.33 -3.2
295,082 + Psest_0280 0.39 -0.1
295,083 - Psest_0280 0.39 -1.8
295,083 - Psest_0280 0.39 -2.1
295,419 - Psest_0280 0.68 -3.4
295,512 + Psest_0280 0.76 -3.0
295,512 + Psest_0280 0.76 -5.2
295,512 + Psest_0280 0.76 -1.6
295,513 - Psest_0280 0.76 -1.8
295,513 - Psest_0280 0.76 -0.5
295,513 - Psest_0280 0.76 -1.8
295,516 + Psest_0280 0.77 -3.3
295,516 + Psest_0280 0.77 -3.0
295,516 + Psest_0280 0.77 -1.3
295,516 + Psest_0280 0.77 -4.8
295,516 + Psest_0280 0.77 -1.6
295,516 + Psest_0280 0.77 -2.6
295,516 + Psest_0280 0.77 -3.9
295,516 + Psest_0280 0.77 -4.6
295,516 + Psest_0280 0.77 -3.9
295,516 + Psest_0280 0.77 -1.8
295,516 + Psest_0280 0.77 -2.9
295,517 - Psest_0280 0.77 -2.8
295,517 - Psest_0280 0.77 -3.4
295,517 - Psest_0280 0.77 -4.4
295,517 - Psest_0280 0.77 +1.1
295,517 - Psest_0280 0.77 -1.6
295,560 + Psest_0280 0.80 -0.9

Or see this region's nucleotide sequence