Experiment: Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0007 and Psest_0008 are separated by 50 nucleotides Psest_0008 and Psest_0009 overlap by 4 nucleotides Psest_0009 and Psest_0010 overlap by 32 nucleotides
Psest_0007: Psest_0007 - diguanylate cyclase (GGDEF) domain, at 8,642 to 10,012
_0007
Psest_0008: Psest_0008 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 10,063 to 10,812
_0008
Psest_0009: Psest_0009 - histidinol-phosphate phosphatase family domain/HAD-superfamily hydrolase, subfamily IIIA, at 10,809 to 11,387
_0009
Psest_0010: Psest_0010 - glycyl-tRNA synthetase, tetrameric type, beta subunit, at 11,356 to 13,410
_0010
Position (kb)
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 9.083 kb on + strand, within Psest_0007 at 9.083 kb on + strand, within Psest_0007 at 9.247 kb on + strand, within Psest_0007 at 9.281 kb on + strand, within Psest_0007 at 9.281 kb on + strand, within Psest_0007 at 9.281 kb on + strand, within Psest_0007 at 9.281 kb on + strand, within Psest_0007 at 9.282 kb on - strand, within Psest_0007 at 9.282 kb on - strand, within Psest_0007 at 9.282 kb on - strand, within Psest_0007 at 9.282 kb on - strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.286 kb on - strand, within Psest_0007 at 9.286 kb on - strand, within Psest_0007 at 9.286 kb on - strand, within Psest_0007 at 9.544 kb on - strand, within Psest_0007 at 9.883 kb on - strand at 9.883 kb on - strand at 10.001 kb on + strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.013 kb on - strand at 10.828 kb on - strand at 10.828 kb on - strand at 10.828 kb on - strand at 11.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml remove 9,083 + Psest_0007 0.32 +1.0 9,083 + Psest_0007 0.32 +0.7 9,247 + Psest_0007 0.44 -1.3 9,281 + Psest_0007 0.47 -0.6 9,281 + Psest_0007 0.47 +0.1 9,281 + Psest_0007 0.47 +1.3 9,281 + Psest_0007 0.47 +1.3 9,282 - Psest_0007 0.47 -1.2 9,282 - Psest_0007 0.47 -0.5 9,282 - Psest_0007 0.47 -1.2 9,282 - Psest_0007 0.47 -1.8 9,285 + Psest_0007 0.47 +2.2 9,285 + Psest_0007 0.47 +0.2 9,285 + Psest_0007 0.47 -0.2 9,285 + Psest_0007 0.47 -0.4 9,285 + Psest_0007 0.47 -1.7 9,285 + Psest_0007 0.47 -0.1 9,285 + Psest_0007 0.47 -0.5 9,286 - Psest_0007 0.47 +1.3 9,286 - Psest_0007 0.47 +1.0 9,286 - Psest_0007 0.47 +0.3 9,544 - Psest_0007 0.66 -1.0 9,883 - +2.3 9,883 - +0.9 10,001 + -0.5 10,002 - +0.1 10,002 - -1.5 10,002 - -3.1 10,002 - -0.5 10,002 - -3.0 10,013 - -1.0 10,828 - +0.1 10,828 - -2.1 10,828 - -0.9 11,358 - +2.0
Or see this region's nucleotide sequence