Experiment: Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0481 and Psest_0482 are separated by 166 nucleotides Psest_0482 and Psest_0483 are separated by 63 nucleotides Psest_0483 and Psest_0484 overlap by 4 nucleotides Psest_0484 and Psest_0485 overlap by 4 nucleotides
Psest_0481: Psest_0481 - Predicted phosphohydrolases, at 530,851 to 531,666
_0481
Psest_0482: Psest_0482 - yqiA-like hydrolase, affects the cell envelope (from data) , at 531,833 to 532,438
_0482
Psest_0483: Psest_0483 - DNA topoisomerase IV, B subunit, proteobacterial, at 532,502 to 534,481
_0483
Psest_0484: Psest_0484 - hypothetical protein, at 534,478 to 535,461
_0484
Psest_0485: Psest_0485 - clan AA aspartic protease, TIGR02281 family, at 535,458 to 535,985
_0485
Position (kb)
532
533
534
535 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 531.662 kb on - strand at 531.738 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.802 kb on + strand at 531.802 kb on + strand at 531.803 kb on - strand at 531.803 kb on - strand at 531.820 kb on - strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 532.056 kb on - strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.142 kb on + strand, within Psest_0482 at 532.166 kb on + strand, within Psest_0482 at 532.292 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.333 kb on + strand, within Psest_0482 at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 534.675 kb on + strand, within Psest_0484 at 534.759 kb on + strand, within Psest_0484 at 534.759 kb on + strand, within Psest_0484 at 534.841 kb on + strand, within Psest_0484 at 535.094 kb on + strand, within Psest_0484 at 535.095 kb on - strand, within Psest_0484 at 535.315 kb on + strand, within Psest_0484 at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml remove 531,662 - -1.5 531,738 + +1.7 531,746 + -0.5 531,746 + -1.8 531,746 + -0.2 531,746 + +0.1 531,746 + -0.2 531,746 + -0.3 531,747 - -1.8 531,747 - +3.8 531,747 - -0.1 531,747 - -1.3 531,747 - -0.6 531,747 - -0.5 531,802 + +0.0 531,802 + -1.9 531,803 - -1.4 531,803 - -1.6 531,820 - -3.4 531,860 + +1.3 531,860 + +1.5 531,860 + -0.8 531,860 + -1.7 531,860 + +0.3 531,860 + -1.1 531,860 + +1.3 531,860 + -1.1 531,860 + -2.9 531,861 - -1.1 531,861 - -0.8 531,861 - -0.7 531,861 - -2.5 531,861 - -1.9 531,861 - -2.4 531,861 - -0.5 531,862 + -3.2 531,862 + -0.2 531,862 + -1.5 531,862 + -3.5 531,862 + -0.4 531,862 + -1.4 531,862 + -0.6 531,862 + -0.9 531,862 + -1.5 531,862 + -1.1 531,862 + -0.5 531,862 + +0.5 531,862 - +1.5 531,863 - +0.1 531,863 - -0.1 531,863 - -0.8 531,863 - -2.9 531,863 - -0.4 531,863 - +0.3 531,863 - -1.6 531,863 - -1.1 531,863 - +0.6 531,863 - -2.5 531,863 - -1.0 531,863 - -0.7 531,863 - -0.2 531,864 + -1.5 531,864 + -1.0 531,864 + -0.1 531,864 + -3.2 531,864 + -1.3 531,864 + -1.3 531,864 + -1.5 531,864 + -0.7 531,864 + -2.1 531,864 + -2.7 531,864 + -2.7 531,864 + -1.7 531,865 - -0.2 531,865 - -0.8 531,865 - -0.3 531,865 - +1.5 531,865 - +0.9 531,865 - -0.1 531,865 - +0.6 531,865 - -1.6 531,865 - -2.4 531,865 - -0.9 531,865 - -1.7 531,865 - -0.9 531,865 - +0.0 532,056 - Psest_0482 0.37 -2.6 532,058 + Psest_0482 0.37 -2.4 532,058 + Psest_0482 0.37 -2.7 532,058 + Psest_0482 0.37 -3.8 532,142 + Psest_0482 0.51 -1.7 532,166 + Psest_0482 0.55 -1.8 532,292 + Psest_0482 0.76 -2.2 532,313 + Psest_0482 0.79 -1.4 532,313 + Psest_0482 0.79 -0.3 532,313 + Psest_0482 0.79 -2.5 532,333 + Psest_0482 0.83 -0.1 532,576 + -0.8 532,576 + -2.0 532,576 + -1.1 532,576 + +1.9 532,576 + +0.6 532,576 + -0.1 532,576 + +0.3 532,576 + +0.7 532,576 + -1.3 532,576 + +1.4 534,675 + Psest_0484 0.20 +0.9 534,759 + Psest_0484 0.29 +0.8 534,759 + Psest_0484 0.29 +2.2 534,841 + Psest_0484 0.37 +1.4 535,094 + Psest_0484 0.63 +3.1 535,095 - Psest_0484 0.63 -0.8 535,315 + Psest_0484 0.85 -1.8 535,371 + +2.6 535,371 + +2.3 535,371 + -1.6 535,371 + +2.3 535,371 + +0.6
Or see this region's nucleotide sequence