Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0375

Experiment: Doxycycline hyclate 0.0002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0374 and Psest_0375 are separated by 70 nucleotidesPsest_0375 and Psest_0376 are separated by 92 nucleotides Psest_0374: Psest_0374 - 5-dehydro-4-deoxyglucarate dehydratase, at 401,090 to 402,001 _0374 Psest_0375: Psest_0375 - NAD-dependent aldehyde dehydrogenases, at 402,072 to 403,514 _0375 Psest_0376: Psest_0376 - glucarate dehydratase, at 403,607 to 404,947 _0376 Position (kb) 402 403 404Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 401.187 kb on - strand, within Psest_0374at 401.187 kb on - strand, within Psest_0374at 401.188 kb on + strand, within Psest_0374at 401.188 kb on + strand, within Psest_0374at 401.189 kb on - strand, within Psest_0374at 401.282 kb on + strand, within Psest_0374at 401.430 kb on - strand, within Psest_0374at 401.430 kb on - strand, within Psest_0374at 401.510 kb on + strand, within Psest_0374at 401.510 kb on + strand, within Psest_0374at 401.510 kb on + strand, within Psest_0374at 401.511 kb on - strand, within Psest_0374at 401.511 kb on - strand, within Psest_0374at 401.511 kb on - strand, within Psest_0374at 401.511 kb on - strand, within Psest_0374at 401.654 kb on + strand, within Psest_0374at 401.655 kb on - strand, within Psest_0374at 401.684 kb on + strand, within Psest_0374at 401.684 kb on + strand, within Psest_0374at 401.684 kb on + strand, within Psest_0374at 401.685 kb on - strand, within Psest_0374at 401.685 kb on - strand, within Psest_0374at 401.685 kb on - strand, within Psest_0374at 401.685 kb on - strand, within Psest_0374at 401.697 kb on - strand, within Psest_0374at 401.720 kb on + strand, within Psest_0374at 401.720 kb on + strand, within Psest_0374at 401.768 kb on + strand, within Psest_0374at 401.769 kb on - strand, within Psest_0374at 401.769 kb on - strand, within Psest_0374at 401.769 kb on - strand, within Psest_0374at 401.769 kb on - strand, within Psest_0374at 401.864 kb on + strand, within Psest_0374at 401.958 kb on - strandat 402.000 kb on - strandat 402.027 kb on - strandat 402.082 kb on + strandat 402.082 kb on + strandat 402.082 kb on + strandat 402.082 kb on + strandat 402.082 kb on + strandat 402.083 kb on - strandat 402.083 kb on - strandat 402.083 kb on - strandat 402.083 kb on - strandat 402.083 kb on - strandat 402.083 kb on - strandat 402.083 kb on - strandat 402.083 kb on - strandat 402.090 kb on + strandat 402.090 kb on + strandat 402.398 kb on + strand, within Psest_0375at 402.399 kb on - strand, within Psest_0375at 402.399 kb on - strand, within Psest_0375at 402.408 kb on + strand, within Psest_0375at 402.408 kb on + strand, within Psest_0375at 402.409 kb on - strand, within Psest_0375at 402.409 kb on - strand, within Psest_0375at 402.409 kb on - strand, within Psest_0375at 402.409 kb on - strand, within Psest_0375at 402.409 kb on - strand, within Psest_0375at 402.960 kb on + strand, within Psest_0375at 402.960 kb on + strand, within Psest_0375at 402.960 kb on + strand, within Psest_0375at 402.960 kb on + strand, within Psest_0375at 402.960 kb on + strand, within Psest_0375at 402.960 kb on + strand, within Psest_0375at 402.960 kb on + strand, within Psest_0375at 402.961 kb on - strand, within Psest_0375at 402.961 kb on - strand, within Psest_0375at 403.083 kb on + strand, within Psest_0375at 403.083 kb on + strand, within Psest_0375at 403.083 kb on + strand, within Psest_0375at 403.158 kb on + strand, within Psest_0375at 403.158 kb on + strand, within Psest_0375at 403.260 kb on + strand, within Psest_0375at 403.260 kb on + strand, within Psest_0375at 403.336 kb on - strand, within Psest_0375at 403.482 kb on + strandat 403.483 kb on - strandat 403.483 kb on - strandat 403.483 kb on - strandat 403.483 kb on - strandat 403.483 kb on - strandat 403.483 kb on - strandat 403.503 kb on + strandat 403.503 kb on + strandat 403.503 kb on + strandat 403.503 kb on + strandat 403.503 kb on + strandat 403.503 kb on + strandat 403.504 kb on - strandat 403.504 kb on - strandat 403.504 kb on - strandat 403.504 kb on - strandat 403.504 kb on - strandat 403.504 kb on - strandat 403.504 kb on - strandat 403.504 kb on - strandat 403.847 kb on + strand, within Psest_0376at 403.847 kb on + strand, within Psest_0376at 403.847 kb on + strand, within Psest_0376at 403.848 kb on - strand, within Psest_0376at 403.848 kb on - strand, within Psest_0376at 404.195 kb on + strand, within Psest_0376at 404.195 kb on + strand, within Psest_0376

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml
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401,187 - Psest_0374 0.11 -0.1
401,187 - Psest_0374 0.11 -2.9
401,188 + Psest_0374 0.11 -2.4
401,188 + Psest_0374 0.11 +1.2
401,189 - Psest_0374 0.11 -0.8
401,282 + Psest_0374 0.21 +2.2
401,430 - Psest_0374 0.37 +3.1
401,430 - Psest_0374 0.37 -0.5
401,510 + Psest_0374 0.46 -0.8
401,510 + Psest_0374 0.46 -3.4
401,510 + Psest_0374 0.46 +0.5
401,511 - Psest_0374 0.46 +1.5
401,511 - Psest_0374 0.46 -0.8
401,511 - Psest_0374 0.46 +2.3
401,511 - Psest_0374 0.46 -0.2
401,654 + Psest_0374 0.62 +2.3
401,655 - Psest_0374 0.62 +1.4
401,684 + Psest_0374 0.65 +0.3
401,684 + Psest_0374 0.65 +2.1
401,684 + Psest_0374 0.65 +1.5
401,685 - Psest_0374 0.65 -1.4
401,685 - Psest_0374 0.65 -0.7
401,685 - Psest_0374 0.65 +0.1
401,685 - Psest_0374 0.65 +3.0
401,697 - Psest_0374 0.67 -1.3
401,720 + Psest_0374 0.69 +1.0
401,720 + Psest_0374 0.69 +0.7
401,768 + Psest_0374 0.74 -1.1
401,769 - Psest_0374 0.74 +0.9
401,769 - Psest_0374 0.74 +2.4
401,769 - Psest_0374 0.74 -1.3
401,769 - Psest_0374 0.74 -1.1
401,864 + Psest_0374 0.85 -1.2
401,958 - -0.3
402,000 - -0.7
402,027 - -0.4
402,082 + +1.4
402,082 + +1.5
402,082 + +2.5
402,082 + +0.3
402,082 + -0.1
402,083 - -0.1
402,083 - +0.3
402,083 - +1.8
402,083 - -3.1
402,083 - -1.0
402,083 - -1.9
402,083 - -0.8
402,083 - -2.6
402,090 + +1.1
402,090 + +1.6
402,398 + Psest_0375 0.23 -0.6
402,399 - Psest_0375 0.23 -0.5
402,399 - Psest_0375 0.23 -1.0
402,408 + Psest_0375 0.23 -1.2
402,408 + Psest_0375 0.23 -1.6
402,409 - Psest_0375 0.23 -1.4
402,409 - Psest_0375 0.23 -1.5
402,409 - Psest_0375 0.23 +0.7
402,409 - Psest_0375 0.23 +2.3
402,409 - Psest_0375 0.23 -0.6
402,960 + Psest_0375 0.62 +1.7
402,960 + Psest_0375 0.62 +1.3
402,960 + Psest_0375 0.62 -0.8
402,960 + Psest_0375 0.62 -1.7
402,960 + Psest_0375 0.62 +0.2
402,960 + Psest_0375 0.62 -3.5
402,960 + Psest_0375 0.62 +2.5
402,961 - Psest_0375 0.62 -0.4
402,961 - Psest_0375 0.62 +0.1
403,083 + Psest_0375 0.70 +0.0
403,083 + Psest_0375 0.70 +0.2
403,083 + Psest_0375 0.70 -1.0
403,158 + Psest_0375 0.75 -0.1
403,158 + Psest_0375 0.75 +0.3
403,260 + Psest_0375 0.82 -1.3
403,260 + Psest_0375 0.82 -1.2
403,336 - Psest_0375 0.88 +0.3
403,482 + +1.2
403,483 - -0.8
403,483 - -0.2
403,483 - +0.8
403,483 - -0.7
403,483 - -0.7
403,483 - +2.2
403,503 + -1.7
403,503 + +0.9
403,503 + +0.0
403,503 + +2.5
403,503 + -0.6
403,503 + +2.6
403,504 - -0.7
403,504 - +4.8
403,504 - -2.3
403,504 - +1.8
403,504 - -1.6
403,504 - -1.0
403,504 - -1.6
403,504 - +1.1
403,847 + Psest_0376 0.18 +1.9
403,847 + Psest_0376 0.18 -0.9
403,847 + Psest_0376 0.18 -2.6
403,848 - Psest_0376 0.18 -0.9
403,848 - Psest_0376 0.18 +1.1
404,195 + Psest_0376 0.44 -1.6
404,195 + Psest_0376 0.44 +0.2

Or see this region's nucleotide sequence