Experiment: Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0319 and Psest_0320 are separated by 174 nucleotides Psest_0320 and Psest_0321 are separated by 12 nucleotides Psest_0321 and Psest_0322 are separated by 55 nucleotides
Psest_0319: Psest_0319 - Predicted ferric reductase, at 342,114 to 343,445
_0319
Psest_0320: Psest_0320 - ABC-type multidrug transport system, ATPase component, at 343,620 to 344,543
_0320
Psest_0321: Psest_0321 - Predicted membrane protein, at 344,556 to 345,317
_0321
Psest_0322: Psest_0322 - hypothetical protein, at 345,373 to 345,708
_0322
Position (kb) 343
344
345 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 342.720 kb on - strand, within Psest_0319 at 342.897 kb on + strand, within Psest_0319 at 342.898 kb on - strand, within Psest_0319 at 342.898 kb on - strand, within Psest_0319 at 342.898 kb on - strand, within Psest_0319 at 342.898 kb on - strand, within Psest_0319 at 342.925 kb on + strand, within Psest_0319 at 342.925 kb on + strand, within Psest_0319 at 342.926 kb on - strand, within Psest_0319 at 342.926 kb on - strand, within Psest_0319 at 342.926 kb on - strand, within Psest_0319 at 343.016 kb on - strand, within Psest_0319 at 343.222 kb on + strand, within Psest_0319 at 343.223 kb on - strand, within Psest_0319 at 343.321 kb on + strand at 343.321 kb on + strand at 343.322 kb on - strand at 343.322 kb on - strand at 343.322 kb on - strand at 343.322 kb on - strand at 343.423 kb on + strand at 343.423 kb on + strand at 343.424 kb on - strand at 343.424 kb on - strand at 343.424 kb on - strand at 343.424 kb on - strand at 343.434 kb on + strand at 343.434 kb on + strand at 343.434 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.496 kb on + strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.497 kb on - strand at 343.579 kb on + strand at 343.580 kb on - strand at 343.583 kb on + strand at 343.659 kb on + strand at 343.659 kb on + strand at 343.660 kb on - strand at 343.660 kb on - strand at 344.196 kb on + strand, within Psest_0320 at 344.196 kb on + strand, within Psest_0320 at 344.197 kb on - strand, within Psest_0320 at 344.197 kb on - strand, within Psest_0320 at 344.197 kb on - strand, within Psest_0320 at 344.197 kb on - strand, within Psest_0320 at 344.197 kb on - strand, within Psest_0320 at 344.197 kb on - strand, within Psest_0320 at 344.197 kb on - strand, within Psest_0320 at 344.565 kb on + strand at 344.583 kb on + strand at 344.583 kb on + strand at 344.583 kb on + strand at 344.584 kb on - strand at 344.584 kb on - strand at 344.584 kb on - strand at 344.584 kb on - strand at 344.658 kb on + strand, within Psest_0321 at 344.658 kb on + strand, within Psest_0321 at 344.659 kb on - strand, within Psest_0321 at 344.659 kb on - strand, within Psest_0321 at 344.659 kb on - strand, within Psest_0321 at 344.668 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.796 kb on + strand, within Psest_0321 at 344.797 kb on + strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.797 kb on - strand, within Psest_0321 at 344.823 kb on + strand, within Psest_0321 at 344.823 kb on + strand, within Psest_0321 at 344.823 kb on + strand, within Psest_0321 at 344.823 kb on + strand, within Psest_0321 at 344.823 kb on + strand, within Psest_0321 at 344.823 kb on + strand, within Psest_0321 at 344.824 kb on - strand, within Psest_0321 at 344.824 kb on - strand, within Psest_0321 at 344.850 kb on + strand, within Psest_0321 at 344.850 kb on + strand, within Psest_0321 at 344.851 kb on - strand, within Psest_0321 at 344.851 kb on - strand, within Psest_0321 at 344.851 kb on - strand, within Psest_0321 at 344.851 kb on - strand, within Psest_0321 at 344.946 kb on + strand, within Psest_0321 at 344.946 kb on + strand, within Psest_0321 at 345.111 kb on + strand, within Psest_0321 at 345.111 kb on + strand, within Psest_0321 at 345.111 kb on + strand, within Psest_0321 at 345.111 kb on + strand, within Psest_0321 at 345.111 kb on + strand, within Psest_0321 at 345.112 kb on - strand, within Psest_0321 at 345.112 kb on - strand, within Psest_0321 at 345.112 kb on - strand, within Psest_0321 at 345.171 kb on + strand, within Psest_0321 at 345.171 kb on + strand, within Psest_0321 at 345.172 kb on - strand, within Psest_0321 at 345.172 kb on - strand, within Psest_0321 at 345.172 kb on - strand, within Psest_0321 at 345.172 kb on - strand, within Psest_0321 at 345.172 kb on - strand, within Psest_0321 at 345.201 kb on + strand, within Psest_0321 at 345.201 kb on + strand, within Psest_0321 at 345.201 kb on + strand, within Psest_0321 at 345.201 kb on + strand, within Psest_0321 at 345.201 kb on + strand, within Psest_0321 at 345.202 kb on - strand, within Psest_0321 at 345.202 kb on - strand, within Psest_0321 at 345.358 kb on + strand at 345.358 kb on + strand at 345.359 kb on - strand at 345.359 kb on - strand at 345.374 kb on + strand at 345.374 kb on + strand at 345.374 kb on + strand at 345.374 kb on + strand at 345.374 kb on + strand at 345.374 kb on + strand at 345.374 kb on + strand at 345.375 kb on - strand at 345.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml remove 342,720 - Psest_0319 0.45 -1.0 342,897 + Psest_0319 0.59 -1.1 342,898 - Psest_0319 0.59 +0.9 342,898 - Psest_0319 0.59 -1.9 342,898 - Psest_0319 0.59 -1.3 342,898 - Psest_0319 0.59 -0.3 342,925 + Psest_0319 0.61 -1.9 342,925 + Psest_0319 0.61 -0.9 342,926 - Psest_0319 0.61 +1.8 342,926 - Psest_0319 0.61 +1.6 342,926 - Psest_0319 0.61 +0.8 343,016 - Psest_0319 0.68 +0.4 343,222 + Psest_0319 0.83 +1.2 343,223 - Psest_0319 0.83 +0.3 343,321 + -1.1 343,321 + +1.1 343,322 - -1.7 343,322 - -0.2 343,322 - -1.1 343,322 - -1.3 343,423 + -0.8 343,423 + +1.8 343,424 - -1.4 343,424 - +1.2 343,424 - -0.9 343,424 - +0.8 343,434 + -2.2 343,434 + -0.5 343,434 + -3.3 343,496 + +0.0 343,496 + +1.1 343,496 + +1.8 343,496 + +2.5 343,496 + +2.7 343,496 + -2.0 343,496 + +1.7 343,496 + -3.6 343,496 + -2.3 343,496 + -1.8 343,496 + +0.0 343,496 + -0.2 343,496 + -0.6 343,496 + -1.8 343,496 + -1.2 343,496 + +0.2 343,496 + -0.5 343,496 + -1.8 343,496 + -0.8 343,496 + +2.2 343,496 + -0.1 343,496 + -2.1 343,496 + -1.1 343,496 + +0.9 343,496 + -2.6 343,496 + +1.6 343,496 + -2.9 343,496 + -0.8 343,496 + +0.5 343,496 + +0.4 343,496 + +1.6 343,496 + -1.9 343,496 + -1.1 343,496 + -0.8 343,496 + -1.1 343,497 - +1.5 343,497 - +0.5 343,497 - +1.1 343,497 - -1.1 343,497 - +0.6 343,497 - -0.9 343,497 - +1.6 343,497 - +0.6 343,497 - +1.8 343,497 - +2.6 343,497 - +0.3 343,497 - +1.2 343,497 - -0.2 343,497 - +1.1 343,497 - +0.8 343,497 - +0.8 343,497 - +0.5 343,497 - +0.0 343,497 - -3.0 343,497 - +2.1 343,497 - +1.1 343,497 - +1.2 343,497 - +0.1 343,497 - -0.6 343,497 - -0.8 343,497 - +0.2 343,497 - -0.5 343,497 - +0.2 343,497 - -1.5 343,497 - -1.1 343,497 - +0.2 343,497 - +3.0 343,579 + -1.9 343,580 - +2.6 343,583 + +2.7 343,659 + +3.8 343,659 + +2.6 343,660 - +2.1 343,660 - +1.5 344,196 + Psest_0320 0.62 +0.7 344,196 + Psest_0320 0.62 +1.1 344,197 - Psest_0320 0.62 +3.2 344,197 - Psest_0320 0.62 +3.4 344,197 - Psest_0320 0.62 +3.8 344,197 - Psest_0320 0.62 +2.0 344,197 - Psest_0320 0.62 -0.7 344,197 - Psest_0320 0.62 +0.4 344,197 - Psest_0320 0.62 -0.4 344,565 + -2.1 344,583 + +1.1 344,583 + +0.8 344,583 + +2.3 344,584 - +1.2 344,584 - -0.8 344,584 - -1.9 344,584 - +1.6 344,658 + Psest_0321 0.13 +1.0 344,658 + Psest_0321 0.13 +0.9 344,659 - Psest_0321 0.14 -0.9 344,659 - Psest_0321 0.14 -2.3 344,659 - Psest_0321 0.14 -2.4 344,668 + Psest_0321 0.15 -0.1 344,796 + Psest_0321 0.31 -1.3 344,796 + Psest_0321 0.31 -0.8 344,796 + Psest_0321 0.31 -2.1 344,796 + Psest_0321 0.31 -1.4 344,796 + Psest_0321 0.31 -1.6 344,796 + Psest_0321 0.31 +2.8 344,796 + Psest_0321 0.31 +0.1 344,796 + Psest_0321 0.31 +1.4 344,796 + Psest_0321 0.31 -1.4 344,796 + Psest_0321 0.31 -0.1 344,796 + Psest_0321 0.31 -1.3 344,796 + Psest_0321 0.31 +2.2 344,796 + Psest_0321 0.31 -0.1 344,796 + Psest_0321 0.31 +1.2 344,796 + Psest_0321 0.31 +0.4 344,796 + Psest_0321 0.31 -0.8 344,796 + Psest_0321 0.31 -0.1 344,796 + Psest_0321 0.31 +1.2 344,796 + Psest_0321 0.31 -1.6 344,797 + Psest_0321 0.32 -0.8 344,797 - Psest_0321 0.32 -0.1 344,797 - Psest_0321 0.32 -1.9 344,797 - Psest_0321 0.32 +1.6 344,797 - Psest_0321 0.32 -1.9 344,797 - Psest_0321 0.32 -0.6 344,797 - Psest_0321 0.32 +0.6 344,797 - Psest_0321 0.32 -0.1 344,797 - Psest_0321 0.32 +2.1 344,797 - Psest_0321 0.32 -0.7 344,797 - Psest_0321 0.32 -0.5 344,797 - Psest_0321 0.32 -2.6 344,797 - Psest_0321 0.32 -2.4 344,797 - Psest_0321 0.32 +0.5 344,797 - Psest_0321 0.32 -1.3 344,797 - Psest_0321 0.32 -0.5 344,823 + Psest_0321 0.35 +1.1 344,823 + Psest_0321 0.35 +1.5 344,823 + Psest_0321 0.35 -1.3 344,823 + Psest_0321 0.35 +0.5 344,823 + Psest_0321 0.35 +0.1 344,823 + Psest_0321 0.35 +0.8 344,824 - Psest_0321 0.35 -0.9 344,824 - Psest_0321 0.35 -1.4 344,850 + Psest_0321 0.39 -1.6 344,850 + Psest_0321 0.39 +0.6 344,851 - Psest_0321 0.39 +4.2 344,851 - Psest_0321 0.39 +0.7 344,851 - Psest_0321 0.39 +2.4 344,851 - Psest_0321 0.39 +1.2 344,946 + Psest_0321 0.51 -0.1 344,946 + Psest_0321 0.51 -1.4 345,111 + Psest_0321 0.73 +0.5 345,111 + Psest_0321 0.73 +0.8 345,111 + Psest_0321 0.73 +0.4 345,111 + Psest_0321 0.73 +0.0 345,111 + Psest_0321 0.73 -0.7 345,112 - Psest_0321 0.73 +1.1 345,112 - Psest_0321 0.73 -2.0 345,112 - Psest_0321 0.73 -1.3 345,171 + Psest_0321 0.81 +1.7 345,171 + Psest_0321 0.81 -0.7 345,172 - Psest_0321 0.81 -0.2 345,172 - Psest_0321 0.81 +2.6 345,172 - Psest_0321 0.81 -1.6 345,172 - Psest_0321 0.81 -1.8 345,172 - Psest_0321 0.81 +1.3 345,201 + Psest_0321 0.85 -1.5 345,201 + Psest_0321 0.85 -0.2 345,201 + Psest_0321 0.85 +1.1 345,201 + Psest_0321 0.85 -0.1 345,201 + Psest_0321 0.85 +0.5 345,202 - Psest_0321 0.85 +1.6 345,202 - Psest_0321 0.85 -1.2 345,358 + +1.7 345,358 + +0.7 345,359 - +1.4 345,359 - +0.3 345,374 + +1.8 345,374 + +1.7 345,374 + +1.5 345,374 + -1.3 345,374 + -1.0 345,374 + -0.9 345,374 + -0.1 345,375 - -1.7 345,375 - -0.8
Or see this region's nucleotide sequence