Experiment: Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0227 and Psest_0228 are separated by 92 nucleotides Psest_0228 and Psest_0229 are separated by 88 nucleotides Psest_0229 and Psest_0230 are separated by 87 nucleotides
Psest_0227: Psest_0227 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 235,979 to 237,373
_0227
Psest_0228: Psest_0228 - Glycerophosphoryl diester phosphodiesterase, at 237,466 to 238,581
_0228
Psest_0229: Psest_0229 - Dihydrofolate reductase, at 238,670 to 239,248
_0229
Psest_0230: Psest_0230 - Uncharacterized BCR, YitT family COG1284., at 239,336 to 239,983
_0230
Position (kb)
237
238
239 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 236.562 kb on + strand, within Psest_0227 at 236.562 kb on + strand, within Psest_0227 at 236.563 kb on - strand, within Psest_0227 at 236.563 kb on - strand, within Psest_0227 at 236.563 kb on - strand, within Psest_0227 at 236.791 kb on + strand, within Psest_0227 at 236.791 kb on + strand, within Psest_0227 at 236.791 kb on + strand, within Psest_0227 at 236.792 kb on - strand, within Psest_0227 at 236.831 kb on + strand, within Psest_0227 at 236.843 kb on + strand at 237.636 kb on - strand, within Psest_0228 at 237.725 kb on + strand, within Psest_0228 at 237.725 kb on + strand, within Psest_0228 at 237.725 kb on + strand, within Psest_0228 at 237.725 kb on + strand, within Psest_0228 at 237.725 kb on + strand, within Psest_0228 at 237.725 kb on + strand, within Psest_0228 at 237.725 kb on + strand, within Psest_0228 at 237.725 kb on + strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.726 kb on - strand, within Psest_0228 at 237.765 kb on + strand, within Psest_0228 at 237.766 kb on - strand, within Psest_0228 at 237.766 kb on - strand, within Psest_0228 at 237.801 kb on - strand, within Psest_0228 at 237.833 kb on + strand, within Psest_0228 at 237.834 kb on - strand, within Psest_0228 at 237.921 kb on + strand, within Psest_0228 at 237.922 kb on - strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.923 kb on + strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.924 kb on - strand, within Psest_0228 at 237.947 kb on + strand, within Psest_0228 at 238.361 kb on + strand, within Psest_0228 at 238.361 kb on + strand, within Psest_0228 at 238.362 kb on - strand, within Psest_0228 at 238.362 kb on - strand, within Psest_0228 at 238.362 kb on - strand, within Psest_0228 at 238.391 kb on + strand, within Psest_0228 at 238.392 kb on - strand, within Psest_0228 at 238.658 kb on + strand at 238.658 kb on + strand at 238.658 kb on + strand at 238.659 kb on - strand at 238.659 kb on - strand at 238.659 kb on - strand at 238.659 kb on - strand at 238.717 kb on + strand at 238.717 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.301 kb on + strand at 239.302 kb on - strand at 239.302 kb on - strand at 239.302 kb on - strand at 239.465 kb on + strand, within Psest_0230 at 239.466 kb on - strand, within Psest_0230 at 239.466 kb on - strand, within Psest_0230 at 239.466 kb on - strand, within Psest_0230 at 239.466 kb on - strand, within Psest_0230 at 239.466 kb on - strand, within Psest_0230 at 239.522 kb on + strand, within Psest_0230 at 239.576 kb on + strand, within Psest_0230 at 239.576 kb on + strand, within Psest_0230 at 239.576 kb on + strand, within Psest_0230 at 239.576 kb on + strand, within Psest_0230 at 239.576 kb on + strand, within Psest_0230 at 239.576 kb on + strand, within Psest_0230 at 239.576 kb on + strand, within Psest_0230 at 239.577 kb on - strand, within Psest_0230
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml remove 236,562 + Psest_0227 0.42 -0.8 236,562 + Psest_0227 0.42 -0.7 236,563 - Psest_0227 0.42 -1.1 236,563 - Psest_0227 0.42 +0.6 236,563 - Psest_0227 0.42 +1.2 236,791 + Psest_0227 0.58 -0.4 236,791 + Psest_0227 0.58 +2.7 236,791 + Psest_0227 0.58 -1.2 236,792 - Psest_0227 0.58 -1.1 236,831 + Psest_0227 0.61 +1.0 236,843 + -2.1 237,636 - Psest_0228 0.15 -0.4 237,725 + Psest_0228 0.23 -1.7 237,725 + Psest_0228 0.23 +0.9 237,725 + Psest_0228 0.23 -0.4 237,725 + Psest_0228 0.23 +1.9 237,725 + Psest_0228 0.23 -0.7 237,725 + Psest_0228 0.23 -0.6 237,725 + Psest_0228 0.23 -1.0 237,725 + Psest_0228 0.23 +1.7 237,726 - Psest_0228 0.23 -0.2 237,726 - Psest_0228 0.23 +1.1 237,726 - Psest_0228 0.23 +2.3 237,726 - Psest_0228 0.23 -1.3 237,726 - Psest_0228 0.23 -0.4 237,726 - Psest_0228 0.23 -0.7 237,726 - Psest_0228 0.23 +1.5 237,726 - Psest_0228 0.23 +1.5 237,726 - Psest_0228 0.23 +0.5 237,726 - Psest_0228 0.23 +1.2 237,726 - Psest_0228 0.23 -0.3 237,765 + Psest_0228 0.27 -3.6 237,766 - Psest_0228 0.27 -1.2 237,766 - Psest_0228 0.27 -1.3 237,801 - Psest_0228 0.30 +1.4 237,833 + Psest_0228 0.33 -0.6 237,834 - Psest_0228 0.33 +0.6 237,921 + Psest_0228 0.41 -1.7 237,922 - Psest_0228 0.41 +2.0 237,923 + Psest_0228 0.41 -1.4 237,923 + Psest_0228 0.41 -0.2 237,923 + Psest_0228 0.41 -0.2 237,923 + Psest_0228 0.41 +1.2 237,923 + Psest_0228 0.41 +0.6 237,923 + Psest_0228 0.41 +0.2 237,923 + Psest_0228 0.41 -0.2 237,923 + Psest_0228 0.41 -1.2 237,923 + Psest_0228 0.41 -1.6 237,924 - Psest_0228 0.41 +1.2 237,924 - Psest_0228 0.41 -0.3 237,924 - Psest_0228 0.41 +3.1 237,924 - Psest_0228 0.41 +0.5 237,924 - Psest_0228 0.41 -2.3 237,924 - Psest_0228 0.41 +2.6 237,924 - Psest_0228 0.41 -1.7 237,924 - Psest_0228 0.41 +3.9 237,924 - Psest_0228 0.41 -0.3 237,924 - Psest_0228 0.41 +2.1 237,924 - Psest_0228 0.41 +0.6 237,924 - Psest_0228 0.41 +0.0 237,924 - Psest_0228 0.41 +0.6 237,924 - Psest_0228 0.41 +1.6 237,947 + Psest_0228 0.43 +2.0 238,361 + Psest_0228 0.80 -1.0 238,361 + Psest_0228 0.80 -1.4 238,362 - Psest_0228 0.80 -1.3 238,362 - Psest_0228 0.80 -1.6 238,362 - Psest_0228 0.80 -1.0 238,391 + Psest_0228 0.83 -0.0 238,392 - Psest_0228 0.83 +0.5 238,658 + +0.1 238,658 + -1.7 238,658 + +2.4 238,659 - +1.2 238,659 - +1.0 238,659 - -0.6 238,659 - -2.2 238,717 + +0.3 238,717 + -0.9 239,301 + +0.2 239,301 + +0.4 239,301 + +1.4 239,301 + -3.6 239,301 + -0.9 239,301 + -0.7 239,301 + -0.2 239,301 + +0.5 239,301 + +1.4 239,301 + +0.6 239,302 - +0.7 239,302 - +1.1 239,302 - +3.0 239,465 + Psest_0230 0.20 +1.1 239,466 - Psest_0230 0.20 -0.4 239,466 - Psest_0230 0.20 -2.3 239,466 - Psest_0230 0.20 -0.8 239,466 - Psest_0230 0.20 -1.4 239,466 - Psest_0230 0.20 -0.6 239,522 + Psest_0230 0.29 +0.6 239,576 + Psest_0230 0.37 +0.2 239,576 + Psest_0230 0.37 +1.1 239,576 + Psest_0230 0.37 -0.0 239,576 + Psest_0230 0.37 +0.5 239,576 + Psest_0230 0.37 -0.7 239,576 + Psest_0230 0.37 -2.5 239,576 + Psest_0230 0.37 -0.3 239,577 - Psest_0230 0.37 -0.3
Or see this region's nucleotide sequence