Experiment: Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2059 and Psest_2060 are separated by 278 nucleotides Psest_2060 and Psest_2061 are separated by 90 nucleotides
Psest_2059: Psest_2059 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 2,183,478 to 2,184,716
_2059
Psest_2060: Psest_2060 - PQQ-dependent dehydrogenase, methanol/ethanol family, at 2,184,995 to 2,186,773
_2060
Psest_2061: Psest_2061 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,186,864 to 2,187,700
_2061
Position (kb)
2184
2185
2186
2187 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2184.097 kb on + strand, within Psest_2059 at 2184.098 kb on - strand, within Psest_2059 at 2184.137 kb on - strand, within Psest_2059 at 2184.223 kb on + strand, within Psest_2059 at 2184.224 kb on - strand, within Psest_2059 at 2184.367 kb on + strand, within Psest_2059 at 2184.368 kb on - strand, within Psest_2059 at 2184.610 kb on + strand at 2184.610 kb on + strand at 2184.610 kb on + strand at 2184.610 kb on + strand at 2184.611 kb on - strand at 2184.611 kb on - strand at 2184.611 kb on - strand at 2184.611 kb on - strand at 2184.611 kb on - strand at 2184.629 kb on - strand at 2184.678 kb on - strand at 2184.777 kb on + strand at 2184.777 kb on + strand at 2184.777 kb on + strand at 2184.868 kb on + strand at 2184.868 kb on + strand at 2184.886 kb on - strand at 2184.967 kb on + strand at 2184.967 kb on + strand at 2184.968 kb on - strand at 2185.286 kb on + strand, within Psest_2060 at 2185.286 kb on + strand, within Psest_2060 at 2185.286 kb on + strand, within Psest_2060 at 2185.286 kb on + strand, within Psest_2060 at 2185.287 kb on - strand, within Psest_2060 at 2185.287 kb on - strand, within Psest_2060 at 2185.352 kb on + strand, within Psest_2060 at 2185.352 kb on + strand, within Psest_2060 at 2185.418 kb on + strand, within Psest_2060 at 2185.419 kb on - strand, within Psest_2060 at 2185.515 kb on - strand, within Psest_2060 at 2185.515 kb on - strand, within Psest_2060 at 2185.619 kb on + strand, within Psest_2060 at 2185.619 kb on + strand, within Psest_2060 at 2185.619 kb on + strand, within Psest_2060 at 2185.679 kb on + strand, within Psest_2060 at 2185.794 kb on - strand, within Psest_2060 at 2185.797 kb on - strand, within Psest_2060 at 2185.880 kb on + strand, within Psest_2060 at 2185.881 kb on - strand, within Psest_2060 at 2185.997 kb on + strand, within Psest_2060 at 2186.057 kb on + strand, within Psest_2060 at 2186.057 kb on + strand, within Psest_2060 at 2186.058 kb on - strand, within Psest_2060 at 2186.058 kb on - strand, within Psest_2060 at 2186.061 kb on + strand, within Psest_2060 at 2186.061 kb on + strand, within Psest_2060 at 2186.152 kb on + strand, within Psest_2060 at 2186.156 kb on + strand, within Psest_2060 at 2186.156 kb on + strand, within Psest_2060 at 2186.156 kb on + strand, within Psest_2060 at 2186.157 kb on - strand, within Psest_2060 at 2186.264 kb on + strand, within Psest_2060 at 2186.288 kb on + strand, within Psest_2060 at 2186.288 kb on + strand, within Psest_2060 at 2186.288 kb on + strand, within Psest_2060 at 2186.288 kb on + strand, within Psest_2060 at 2186.288 kb on + strand, within Psest_2060 at 2186.289 kb on - strand, within Psest_2060 at 2186.289 kb on - strand, within Psest_2060 at 2186.289 kb on - strand, within Psest_2060 at 2186.289 kb on - strand, within Psest_2060 at 2186.289 kb on - strand, within Psest_2060 at 2186.289 kb on - strand, within Psest_2060 at 2186.357 kb on + strand, within Psest_2060 at 2186.399 kb on + strand, within Psest_2060 at 2186.399 kb on + strand, within Psest_2060 at 2186.400 kb on - strand, within Psest_2060 at 2186.400 kb on - strand, within Psest_2060 at 2186.400 kb on - strand, within Psest_2060 at 2186.400 kb on - strand, within Psest_2060 at 2186.400 kb on - strand, within Psest_2060 at 2186.462 kb on + strand, within Psest_2060 at 2186.463 kb on - strand, within Psest_2060 at 2186.463 kb on - strand, within Psest_2060 at 2186.463 kb on - strand, within Psest_2060 at 2186.532 kb on - strand, within Psest_2060 at 2186.636 kb on + strand at 2186.636 kb on + strand at 2186.636 kb on + strand at 2186.636 kb on + strand at 2186.636 kb on + strand at 2186.637 kb on - strand at 2186.941 kb on - strand at 2187.027 kb on + strand, within Psest_2061 at 2187.027 kb on + strand, within Psest_2061 at 2187.028 kb on - strand, within Psest_2061 at 2187.028 kb on - strand, within Psest_2061 at 2187.028 kb on - strand, within Psest_2061 at 2187.172 kb on - strand, within Psest_2061 at 2187.172 kb on - strand, within Psest_2061 at 2187.172 kb on - strand, within Psest_2061 at 2187.172 kb on - strand, within Psest_2061 at 2187.433 kb on - strand, within Psest_2061 at 2187.486 kb on + strand, within Psest_2061 at 2187.754 kb on + strand at 2187.754 kb on + strand at 2187.754 kb on + strand at 2187.755 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml remove 2,184,097 + Psest_2059 0.50 +0.3 2,184,098 - Psest_2059 0.50 -0.5 2,184,137 - Psest_2059 0.53 -0.9 2,184,223 + Psest_2059 0.60 -2.0 2,184,224 - Psest_2059 0.60 +0.3 2,184,367 + Psest_2059 0.72 -0.7 2,184,368 - Psest_2059 0.72 +1.2 2,184,610 + -1.1 2,184,610 + -3.7 2,184,610 + +0.8 2,184,610 + +0.6 2,184,611 - +0.4 2,184,611 - +1.0 2,184,611 - -0.0 2,184,611 - +0.2 2,184,611 - -2.9 2,184,629 - -0.3 2,184,678 - +1.2 2,184,777 + +0.6 2,184,777 + +0.8 2,184,777 + -0.4 2,184,868 + +0.4 2,184,868 + +0.6 2,184,886 - -0.1 2,184,967 + +0.7 2,184,967 + +1.2 2,184,968 - -0.5 2,185,286 + Psest_2060 0.16 -1.3 2,185,286 + Psest_2060 0.16 -1.7 2,185,286 + Psest_2060 0.16 +1.2 2,185,286 + Psest_2060 0.16 +0.9 2,185,287 - Psest_2060 0.16 -0.5 2,185,287 - Psest_2060 0.16 +2.2 2,185,352 + Psest_2060 0.20 +0.2 2,185,352 + Psest_2060 0.20 +0.1 2,185,418 + Psest_2060 0.24 +1.1 2,185,419 - Psest_2060 0.24 -0.9 2,185,515 - Psest_2060 0.29 -3.4 2,185,515 - Psest_2060 0.29 +0.7 2,185,619 + Psest_2060 0.35 -2.2 2,185,619 + Psest_2060 0.35 -0.4 2,185,619 + Psest_2060 0.35 +3.2 2,185,679 + Psest_2060 0.38 +0.5 2,185,794 - Psest_2060 0.45 -0.9 2,185,797 - Psest_2060 0.45 +0.0 2,185,880 + Psest_2060 0.50 -1.8 2,185,881 - Psest_2060 0.50 -0.9 2,185,997 + Psest_2060 0.56 +2.0 2,186,057 + Psest_2060 0.60 -2.2 2,186,057 + Psest_2060 0.60 +0.1 2,186,058 - Psest_2060 0.60 +0.8 2,186,058 - Psest_2060 0.60 -0.9 2,186,061 + Psest_2060 0.60 +0.3 2,186,061 + Psest_2060 0.60 -0.0 2,186,152 + Psest_2060 0.65 -2.9 2,186,156 + Psest_2060 0.65 +0.1 2,186,156 + Psest_2060 0.65 +1.5 2,186,156 + Psest_2060 0.65 -1.0 2,186,157 - Psest_2060 0.65 +0.2 2,186,264 + Psest_2060 0.71 +0.4 2,186,288 + Psest_2060 0.73 +0.9 2,186,288 + Psest_2060 0.73 +1.0 2,186,288 + Psest_2060 0.73 -0.6 2,186,288 + Psest_2060 0.73 +2.1 2,186,288 + Psest_2060 0.73 +1.6 2,186,289 - Psest_2060 0.73 +1.3 2,186,289 - Psest_2060 0.73 -1.4 2,186,289 - Psest_2060 0.73 +0.3 2,186,289 - Psest_2060 0.73 +2.4 2,186,289 - Psest_2060 0.73 +1.2 2,186,289 - Psest_2060 0.73 +0.5 2,186,357 + Psest_2060 0.77 +1.6 2,186,399 + Psest_2060 0.79 -1.2 2,186,399 + Psest_2060 0.79 +0.3 2,186,400 - Psest_2060 0.79 +3.9 2,186,400 - Psest_2060 0.79 -0.8 2,186,400 - Psest_2060 0.79 -0.2 2,186,400 - Psest_2060 0.79 -2.0 2,186,400 - Psest_2060 0.79 +1.2 2,186,462 + Psest_2060 0.82 +2.3 2,186,463 - Psest_2060 0.83 -1.1 2,186,463 - Psest_2060 0.83 +3.4 2,186,463 - Psest_2060 0.83 -0.1 2,186,532 - Psest_2060 0.86 +2.0 2,186,636 + -0.6 2,186,636 + +1.9 2,186,636 + -1.0 2,186,636 + -0.5 2,186,636 + -1.9 2,186,637 - -0.2 2,186,941 - +2.6 2,187,027 + Psest_2061 0.19 +1.6 2,187,027 + Psest_2061 0.19 -1.0 2,187,028 - Psest_2061 0.20 -2.7 2,187,028 - Psest_2061 0.20 -2.2 2,187,028 - Psest_2061 0.20 -0.8 2,187,172 - Psest_2061 0.37 -1.5 2,187,172 - Psest_2061 0.37 -0.3 2,187,172 - Psest_2061 0.37 -2.3 2,187,172 - Psest_2061 0.37 +0.7 2,187,433 - Psest_2061 0.68 +1.7 2,187,486 + Psest_2061 0.74 -1.1 2,187,754 + +1.4 2,187,754 + -0.6 2,187,754 + +1.2 2,187,755 - -0.6
Or see this region's nucleotide sequence