Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0062

Experiment: Urea (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0059 and Psest_0060 are separated by 2 nucleotidesPsest_0060 and Psest_0061 are separated by 118 nucleotidesPsest_0061 and Psest_0062 are separated by 175 nucleotidesPsest_0062 and Psest_0063 overlap by 4 nucleotides Psest_0059: Psest_0059 - hypothetical protein, at 60,782 to 61,153 _0059 Psest_0060: Psest_0060 - Predicted Fe-S protein, at 61,156 to 61,338 _0060 Psest_0061: Psest_0061 - hypothetical protein, at 61,457 to 61,786 _0061 Psest_0062: Psest_0062 - tryptophan synthase, alpha subunit, at 61,962 to 62,771 _0062 Psest_0063: Psest_0063 - tryptophan synthase, beta subunit, at 62,768 to 63,979 _0063 Position (kb) 61 62 63Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 61.215 kb on + strand, within Psest_0060at 61.216 kb on - strand, within Psest_0060at 61.290 kb on - strand, within Psest_0060at 61.416 kb on + strandat 61.417 kb on - strandat 61.530 kb on + strand, within Psest_0061at 61.530 kb on + strand, within Psest_0061at 61.530 kb on + strand, within Psest_0061at 61.531 kb on - strand, within Psest_0061at 61.641 kb on + strand, within Psest_0061at 61.653 kb on + strand, within Psest_0061at 61.896 kb on + strandat 61.897 kb on - strandat 61.900 kb on + strandat 61.900 kb on + strandat 61.900 kb on + strandat 61.901 kb on - strandat 61.901 kb on - strandat 61.901 kb on - strandat 61.901 kb on - strandat 61.901 kb on - strandat 61.980 kb on + strandat 61.980 kb on + strandat 61.981 kb on - strandat 62.245 kb on + strand, within Psest_0062at 62.245 kb on + strand, within Psest_0062at 62.245 kb on + strand, within Psest_0062at 62.245 kb on + strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.248 kb on + strand, within Psest_0062at 62.249 kb on - strand, within Psest_0062at 62.249 kb on - strand, within Psest_0062at 62.249 kb on - strand, within Psest_0062at 62.464 kb on + strand, within Psest_0062at 62.464 kb on + strand, within Psest_0062at 62.464 kb on + strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.684 kb on - strand, within Psest_0062at 62.992 kb on - strand, within Psest_0063at 62.992 kb on - strand, within Psest_0063at 63.405 kb on + strand, within Psest_0063at 63.405 kb on + strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.564 kb on + strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.742 kb on - strand, within Psest_0063at 63.742 kb on - strand, within Psest_0063at 63.742 kb on - strand, within Psest_0063at 63.742 kb on - strand, within Psest_0063

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Per-strain Table

Position Strand Gene LocusTag Fraction Urea (N)
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61,215 + Psest_0060 0.32 -0.0
61,216 - Psest_0060 0.33 +0.1
61,290 - Psest_0060 0.73 -1.7
61,416 + +1.1
61,417 - +0.4
61,530 + Psest_0061 0.22 -1.1
61,530 + Psest_0061 0.22 -1.1
61,530 + Psest_0061 0.22 +1.1
61,531 - Psest_0061 0.22 +2.1
61,641 + Psest_0061 0.56 -0.4
61,653 + Psest_0061 0.59 -0.1
61,896 + -0.4
61,897 - -0.1
61,900 + +0.1
61,900 + +1.6
61,900 + +0.6
61,901 - -0.3
61,901 - +0.4
61,901 - -1.4
61,901 - +1.4
61,901 - -1.0
61,980 + -0.7
61,980 + -1.7
61,981 - -1.3
62,245 + Psest_0062 0.35 -4.1
62,245 + Psest_0062 0.35 -4.1
62,245 + Psest_0062 0.35 -4.3
62,245 + Psest_0062 0.35 -4.1
62,246 - Psest_0062 0.35 -1.6
62,246 - Psest_0062 0.35 -2.1
62,246 - Psest_0062 0.35 -1.4
62,246 - Psest_0062 0.35 -3.2
62,246 - Psest_0062 0.35 -3.9
62,248 + Psest_0062 0.35 -5.7
62,249 - Psest_0062 0.35 -2.0
62,249 - Psest_0062 0.35 -2.7
62,249 - Psest_0062 0.35 -3.1
62,464 + Psest_0062 0.62 -5.8
62,464 + Psest_0062 0.62 -5.4
62,464 + Psest_0062 0.62 -6.2
62,465 - Psest_0062 0.62 -4.0
62,465 - Psest_0062 0.62 -4.0
62,465 - Psest_0062 0.62 -5.8
62,465 - Psest_0062 0.62 -5.2
62,465 - Psest_0062 0.62 -3.9
62,465 - Psest_0062 0.62 -3.0
62,465 - Psest_0062 0.62 -1.4
62,465 - Psest_0062 0.62 -3.7
62,465 - Psest_0062 0.62 -4.6
62,465 - Psest_0062 0.62 -2.1
62,465 - Psest_0062 0.62 -5.0
62,465 - Psest_0062 0.62 -2.6
62,684 - Psest_0062 0.89 -5.0
62,992 - Psest_0063 0.18 -2.7
62,992 - Psest_0063 0.18 -5.2
63,405 + Psest_0063 0.53 -2.1
63,405 + Psest_0063 0.53 -3.7
63,406 - Psest_0063 0.53 -3.3
63,406 - Psest_0063 0.53 -4.0
63,406 - Psest_0063 0.53 -5.4
63,406 - Psest_0063 0.53 -4.5
63,406 - Psest_0063 0.53 -2.1
63,406 - Psest_0063 0.53 -2.1
63,406 - Psest_0063 0.53 -2.6
63,406 - Psest_0063 0.53 -3.2
63,564 + Psest_0063 0.66 -4.2
63,565 - Psest_0063 0.66 -3.7
63,565 - Psest_0063 0.66 -4.5
63,565 - Psest_0063 0.66 -3.6
63,565 - Psest_0063 0.66 -3.0
63,565 - Psest_0063 0.66 -3.7
63,741 + Psest_0063 0.80 -4.0
63,741 + Psest_0063 0.80 -2.7
63,741 + Psest_0063 0.80 -1.1
63,741 + Psest_0063 0.80 -2.7
63,741 + Psest_0063 0.80 -2.9
63,742 - Psest_0063 0.80 -5.8
63,742 - Psest_0063 0.80 -3.5
63,742 - Psest_0063 0.80 -1.6
63,742 - Psest_0063 0.80 -0.2

Or see this region's nucleotide sequence