Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2991 and Psest_2992 are separated by 72 nucleotides Psest_2992 and Psest_2993 are separated by 112 nucleotides Psest_2993 and Psest_2994 are separated by 90 nucleotides Psest_2994 and Psest_2995 overlap by 4 nucleotides
Psest_2991: Psest_2991 - hypothetical protein, at 3,182,268 to 3,183,488
_2991
Psest_2992: Psest_2992 - Uncharacterized conserved protein, at 3,183,561 to 3,184,010
_2992
Psest_2993: Psest_2993 - hypothetical protein, at 3,184,123 to 3,184,527
_2993
Psest_2994: Psest_2994 - Uncharacterized protein conserved in bacteria, at 3,184,618 to 3,184,911
_2994
Psest_2995: Psest_2995 - ribonuclease D, at 3,184,908 to 3,186,032
_2995
Position (kb)
3183
3184
3185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.591 kb on - strand, within Psest_2991 at 3182.722 kb on + strand, within Psest_2991 at 3182.722 kb on + strand, within Psest_2991 at 3182.723 kb on - strand, within Psest_2991 at 3182.723 kb on - strand, within Psest_2991 at 3182.894 kb on + strand, within Psest_2991 at 3183.295 kb on + strand, within Psest_2991 at 3183.296 kb on - strand, within Psest_2991 at 3183.298 kb on + strand, within Psest_2991 at 3183.526 kb on + strand at 3183.526 kb on + strand at 3183.526 kb on + strand at 3183.527 kb on - strand at 3183.527 kb on - strand at 3183.556 kb on + strand at 3183.556 kb on + strand at 3183.556 kb on + strand at 3183.557 kb on - strand at 3183.557 kb on - strand at 3183.557 kb on - strand at 3183.557 kb on - strand at 3183.557 kb on - strand at 3183.707 kb on + strand, within Psest_2992 at 3183.872 kb on - strand, within Psest_2992 at 3183.872 kb on - strand, within Psest_2992 at 3183.872 kb on - strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.875 kb on - strand, within Psest_2992 at 3183.875 kb on - strand, within Psest_2992 at 3183.875 kb on - strand, within Psest_2992 at 3184.016 kb on + strand at 3184.016 kb on + strand at 3184.016 kb on + strand at 3184.016 kb on + strand at 3184.017 kb on - strand at 3184.017 kb on - strand at 3184.489 kb on + strand at 3184.541 kb on - strand at 3184.564 kb on + strand at 3184.583 kb on - strand at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.856 kb on + strand, within Psest_2994 at 3184.857 kb on - strand, within Psest_2994 at 3184.857 kb on - strand, within Psest_2994 at 3184.857 kb on - strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.888 kb on - strand at 3184.888 kb on - strand at 3184.889 kb on + strand at 3184.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,182,590 + Psest_2991 0.26 -1.4 3,182,590 + Psest_2991 0.26 -0.1 3,182,590 + Psest_2991 0.26 +0.7 3,182,590 + Psest_2991 0.26 +0.9 3,182,590 + Psest_2991 0.26 +0.7 3,182,590 + Psest_2991 0.26 +1.0 3,182,590 + Psest_2991 0.26 -0.9 3,182,591 - Psest_2991 0.26 -0.1 3,182,722 + Psest_2991 0.37 -1.2 3,182,722 + Psest_2991 0.37 +0.1 3,182,723 - Psest_2991 0.37 +0.3 3,182,723 - Psest_2991 0.37 +0.3 3,182,894 + Psest_2991 0.51 -0.5 3,183,295 + Psest_2991 0.84 +0.2 3,183,296 - Psest_2991 0.84 +1.2 3,183,298 + Psest_2991 0.84 +0.9 3,183,526 + -0.3 3,183,526 + -1.5 3,183,526 + +0.1 3,183,527 - -0.8 3,183,527 - +0.5 3,183,556 + +0.8 3,183,556 + -0.4 3,183,556 + +0.3 3,183,557 - +1.5 3,183,557 - -0.8 3,183,557 - +0.2 3,183,557 - -1.5 3,183,557 - +0.7 3,183,707 + Psest_2992 0.32 -0.8 3,183,872 - Psest_2992 0.69 +2.5 3,183,872 - Psest_2992 0.69 +0.9 3,183,872 - Psest_2992 0.69 -0.6 3,183,874 + Psest_2992 0.70 +0.4 3,183,874 + Psest_2992 0.70 -0.8 3,183,874 + Psest_2992 0.70 -0.7 3,183,874 + Psest_2992 0.70 -0.6 3,183,874 + Psest_2992 0.70 -1.3 3,183,874 + Psest_2992 0.70 +1.0 3,183,875 - Psest_2992 0.70 -0.0 3,183,875 - Psest_2992 0.70 -1.2 3,183,875 - Psest_2992 0.70 -0.5 3,184,016 + +2.2 3,184,016 + +1.1 3,184,016 + -1.3 3,184,016 + +0.4 3,184,017 - +0.8 3,184,017 - -3.0 3,184,489 + +2.0 3,184,541 - -0.0 3,184,564 + +0.0 3,184,583 - +0.7 3,184,667 + Psest_2994 0.17 +0.8 3,184,667 + Psest_2994 0.17 -0.3 3,184,667 + Psest_2994 0.17 -0.6 3,184,667 + Psest_2994 0.17 -0.3 3,184,667 + Psest_2994 0.17 +0.1 3,184,667 + Psest_2994 0.17 -0.8 3,184,667 + Psest_2994 0.17 -0.4 3,184,668 - Psest_2994 0.17 -0.9 3,184,668 - Psest_2994 0.17 -1.2 3,184,668 - Psest_2994 0.17 +0.8 3,184,668 - Psest_2994 0.17 -0.6 3,184,668 - Psest_2994 0.17 +0.3 3,184,668 - Psest_2994 0.17 +1.7 3,184,856 + Psest_2994 0.81 -1.5 3,184,857 - Psest_2994 0.81 +0.2 3,184,857 - Psest_2994 0.81 +0.9 3,184,857 - Psest_2994 0.81 +0.2 3,184,862 + Psest_2994 0.83 +0.5 3,184,862 + Psest_2994 0.83 -0.6 3,184,862 + Psest_2994 0.83 -0.3 3,184,862 + Psest_2994 0.83 +0.8 3,184,862 + Psest_2994 0.83 +0.2 3,184,863 - Psest_2994 0.83 -1.8 3,184,863 - Psest_2994 0.83 +2.7 3,184,863 - Psest_2994 0.83 -0.1 3,184,863 - Psest_2994 0.83 +1.0 3,184,863 - Psest_2994 0.83 +0.2 3,184,863 - Psest_2994 0.83 -0.3 3,184,863 - Psest_2994 0.83 -1.6 3,184,863 - Psest_2994 0.83 -0.8 3,184,863 - Psest_2994 0.83 +0.5 3,184,863 - Psest_2994 0.83 -2.9 3,184,863 - Psest_2994 0.83 -2.3 3,184,863 - Psest_2994 0.83 +1.2 3,184,888 - -0.0 3,184,888 - +1.0 3,184,889 + -1.3 3,184,889 + +0.8
Or see this region's nucleotide sequence