Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3048

Experiment: nitrite (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_3047 and Psest_3048 are separated by 63 nucleotidesPsest_3048 and Psest_3049 overlap by 11 nucleotidesPsest_3049 and Psest_3050 are separated by 80 nucleotidesPsest_3050 and Psest_3051 are separated by 95 nucleotides Psest_3047: Psest_3047 - methionyl-tRNA synthetase/methionyl-tRNA synthetase C-terminal region/beta chain, at 3,229,308 to 3,231,350 _3047 Psest_3048: Psest_3048 - hypothetical protein, at 3,231,414 to 3,231,626 _3048 Psest_3049: Psest_3049 - pseudouridine synthase, at 3,231,616 to 3,232,308 _3049 Psest_3050: Psest_3050 - tRNA_Gly_CCC, at 3,232,389 to 3,232,462 _3050 Psest_3051: Psest_3051 - Predicted Fe-S-cluster oxidoreductase, at 3,232,558 to 3,233,229 _3051 Position (kb) 3231 3232Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3231.001 kb on + strand, within Psest_3047at 3231.063 kb on - strand, within Psest_3047at 3231.348 kb on + strandat 3231.349 kb on - strandat 3231.427 kb on + strandat 3231.427 kb on + strandat 3231.428 kb on - strandat 3231.428 kb on - strandat 3231.521 kb on + strand, within Psest_3048at 3231.521 kb on + strand, within Psest_3048at 3231.522 kb on - strand, within Psest_3048at 3231.813 kb on + strand, within Psest_3049at 3231.814 kb on - strand, within Psest_3049at 3231.940 kb on - strand, within Psest_3049at 3232.009 kb on + strand, within Psest_3049at 3232.009 kb on + strand, within Psest_3049at 3232.010 kb on - strand, within Psest_3049at 3232.010 kb on - strand, within Psest_3049at 3232.010 kb on - strand, within Psest_3049at 3232.010 kb on - strand, within Psest_3049at 3232.072 kb on + strand, within Psest_3049at 3232.072 kb on + strand, within Psest_3049at 3232.072 kb on + strand, within Psest_3049at 3232.072 kb on + strand, within Psest_3049at 3232.072 kb on + strand, within Psest_3049at 3232.072 kb on + strand, within Psest_3049at 3232.073 kb on - strand, within Psest_3049at 3232.073 kb on - strand, within Psest_3049at 3232.073 kb on - strand, within Psest_3049at 3232.073 kb on - strand, within Psest_3049at 3232.073 kb on - strand, within Psest_3049at 3232.073 kb on - strand, within Psest_3049at 3232.163 kb on - strand, within Psest_3049at 3232.399 kb on + strand, within Psest_3050at 3232.399 kb on + strand, within Psest_3050at 3232.399 kb on + strand, within Psest_3050at 3232.400 kb on - strand, within Psest_3050at 3232.400 kb on - strand, within Psest_3050at 3232.402 kb on - strand, within Psest_3050at 3232.411 kb on - strand, within Psest_3050at 3232.560 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite (N)
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3,231,001 + Psest_3047 0.83 +1.4
3,231,063 - Psest_3047 0.86 -1.3
3,231,348 + -2.0
3,231,349 - +0.7
3,231,427 + +0.7
3,231,427 + +1.9
3,231,428 - -0.3
3,231,428 - -1.5
3,231,521 + Psest_3048 0.50 -0.4
3,231,521 + Psest_3048 0.50 +2.4
3,231,522 - Psest_3048 0.51 +0.6
3,231,813 + Psest_3049 0.28 -0.6
3,231,814 - Psest_3049 0.29 -0.8
3,231,940 - Psest_3049 0.47 -0.0
3,232,009 + Psest_3049 0.57 -0.2
3,232,009 + Psest_3049 0.57 -1.3
3,232,010 - Psest_3049 0.57 +0.3
3,232,010 - Psest_3049 0.57 +0.9
3,232,010 - Psest_3049 0.57 +0.8
3,232,010 - Psest_3049 0.57 -1.3
3,232,072 + Psest_3049 0.66 -0.2
3,232,072 + Psest_3049 0.66 -0.1
3,232,072 + Psest_3049 0.66 -1.6
3,232,072 + Psest_3049 0.66 -1.5
3,232,072 + Psest_3049 0.66 +0.0
3,232,072 + Psest_3049 0.66 +2.6
3,232,073 - Psest_3049 0.66 +0.5
3,232,073 - Psest_3049 0.66 +0.2
3,232,073 - Psest_3049 0.66 -0.5
3,232,073 - Psest_3049 0.66 +0.1
3,232,073 - Psest_3049 0.66 -0.3
3,232,073 - Psest_3049 0.66 -1.3
3,232,163 - Psest_3049 0.79 +0.1
3,232,399 + Psest_3050 0.14 -1.1
3,232,399 + Psest_3050 0.14 -0.4
3,232,399 + Psest_3050 0.14 +0.7
3,232,400 - Psest_3050 0.15 +2.4
3,232,400 - Psest_3050 0.15 -0.3
3,232,402 - Psest_3050 0.18 +0.1
3,232,411 - Psest_3050 0.30 +1.6
3,232,560 - -2.9

Or see this region's nucleotide sequence