Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2808 and Psest_2809 are separated by 19 nucleotides Psest_2809 and Psest_2810 overlap by 4 nucleotides Psest_2810 and Psest_2811 overlap by 1 nucleotides
Psest_2808: Psest_2808 - Predicted membrane protein, at 2,997,477 to 2,998,412
_2808
Psest_2809: Psest_2809 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 2,998,432 to 2,999,109
_2809
Psest_2810: Psest_2810 - 5'/3'-nucleotidase SurE, at 2,999,106 to 2,999,855
_2810
Psest_2811: Psest_2811 - Uncharacterized conserved protein, at 2,999,855 to 3,000,895
_2811
Position (kb)
2999
3000 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2998.469 kb on - strand at 2998.638 kb on - strand, within Psest_2809 at 2998.893 kb on + strand, within Psest_2809 at 2998.893 kb on + strand, within Psest_2809 at 2998.931 kb on + strand, within Psest_2809 at 2998.931 kb on + strand, within Psest_2809 at 2999.035 kb on + strand, within Psest_2809 at 2999.035 kb on + strand, within Psest_2809 at 2999.036 kb on - strand, within Psest_2809 at 2999.188 kb on - strand, within Psest_2810 at 2999.188 kb on - strand, within Psest_2810 at 2999.191 kb on + strand, within Psest_2810 at 2999.191 kb on + strand, within Psest_2810 at 2999.192 kb on - strand, within Psest_2810 at 2999.264 kb on - strand, within Psest_2810 at 2999.393 kb on + strand, within Psest_2810 at 2999.393 kb on + strand, within Psest_2810 at 2999.393 kb on + strand, within Psest_2810 at 2999.482 kb on - strand, within Psest_2810 at 2999.604 kb on - strand, within Psest_2810 at 2999.604 kb on - strand, within Psest_2810 at 2999.604 kb on - strand, within Psest_2810 at 2999.798 kb on - strand at 2999.821 kb on + strand at 2999.821 kb on + strand at 2999.822 kb on - strand at 2999.822 kb on - strand at 2999.822 kb on - strand at 2999.849 kb on + strand at 2999.850 kb on - strand at 3000.084 kb on - strand, within Psest_2811 at 3000.273 kb on - strand, within Psest_2811 at 3000.273 kb on - strand, within Psest_2811 at 3000.273 kb on - strand, within Psest_2811 at 3000.278 kb on - strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.292 kb on + strand, within Psest_2811 at 3000.292 kb on + strand, within Psest_2811 at 3000.293 kb on - strand, within Psest_2811 at 3000.357 kb on + strand, within Psest_2811 at 3000.414 kb on + strand, within Psest_2811 at 3000.414 kb on + strand, within Psest_2811 at 3000.414 kb on + strand, within Psest_2811 at 3000.415 kb on - strand, within Psest_2811 at 3000.669 kb on + strand, within Psest_2811 at 3000.669 kb on + strand, within Psest_2811 at 3000.670 kb on - strand, within Psest_2811 at 3000.837 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 2,998,469 - -0.7 2,998,638 - Psest_2809 0.30 -0.4 2,998,893 + Psest_2809 0.68 -0.5 2,998,893 + Psest_2809 0.68 -1.7 2,998,931 + Psest_2809 0.74 -1.0 2,998,931 + Psest_2809 0.74 -1.7 2,999,035 + Psest_2809 0.89 -0.4 2,999,035 + Psest_2809 0.89 -1.6 2,999,036 - Psest_2809 0.89 -2.8 2,999,188 - Psest_2810 0.11 -0.7 2,999,188 - Psest_2810 0.11 -1.5 2,999,191 + Psest_2810 0.11 -2.2 2,999,191 + Psest_2810 0.11 -0.5 2,999,192 - Psest_2810 0.11 -0.4 2,999,264 - Psest_2810 0.21 -1.0 2,999,393 + Psest_2810 0.38 -2.0 2,999,393 + Psest_2810 0.38 -1.5 2,999,393 + Psest_2810 0.38 -2.3 2,999,482 - Psest_2810 0.50 -2.7 2,999,604 - Psest_2810 0.66 -0.7 2,999,604 - Psest_2810 0.66 +0.0 2,999,604 - Psest_2810 0.66 -1.4 2,999,798 - -1.0 2,999,821 + -1.2 2,999,821 + -1.0 2,999,822 - +0.3 2,999,822 - -1.2 2,999,822 - -0.8 2,999,849 + -1.0 2,999,850 - +0.0 3,000,084 - Psest_2811 0.22 -1.1 3,000,273 - Psest_2811 0.40 -1.1 3,000,273 - Psest_2811 0.40 -2.4 3,000,273 - Psest_2811 0.40 +0.2 3,000,278 - Psest_2811 0.41 +1.7 3,000,288 + Psest_2811 0.42 +0.2 3,000,288 + Psest_2811 0.42 +0.2 3,000,288 + Psest_2811 0.42 -1.2 3,000,288 + Psest_2811 0.42 -1.2 3,000,288 + Psest_2811 0.42 -0.5 3,000,289 - Psest_2811 0.42 -0.0 3,000,289 - Psest_2811 0.42 -0.7 3,000,289 - Psest_2811 0.42 -1.0 3,000,289 - Psest_2811 0.42 -1.8 3,000,289 - Psest_2811 0.42 -0.4 3,000,289 - Psest_2811 0.42 -1.2 3,000,289 - Psest_2811 0.42 +0.4 3,000,292 + Psest_2811 0.42 +1.0 3,000,292 + Psest_2811 0.42 -0.5 3,000,293 - Psest_2811 0.42 +0.6 3,000,357 + Psest_2811 0.48 -0.8 3,000,414 + Psest_2811 0.54 +0.3 3,000,414 + Psest_2811 0.54 +0.5 3,000,414 + Psest_2811 0.54 +0.4 3,000,415 - Psest_2811 0.54 +0.1 3,000,669 + Psest_2811 0.78 -0.1 3,000,669 + Psest_2811 0.78 -0.2 3,000,670 - Psest_2811 0.78 +0.3 3,000,837 - -0.6
Or see this region's nucleotide sequence