Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2279 and Psest_2280 are separated by 85 nucleotides Psest_2280 and Psest_2281 overlap by 20 nucleotides Psest_2281 and Psest_2282 overlap by 8 nucleotides Psest_2282 and Psest_2283 are separated by 102 nucleotides
Psest_2279: Psest_2279 - coenzyme PQQ biosynthesis protein E, at 2,427,544 to 2,428,689
_2279
Psest_2280: Psest_2280 - Predicted membrane protein, at 2,428,775 to 2,429,278
_2280
Psest_2281: Psest_2281 - Phosphoserine aminotransferase, at 2,429,259 to 2,430,359
_2281
Psest_2282: Psest_2282 - Dihydroorotate dehydrogenase, at 2,430,352 to 2,431,197
_2282
Psest_2283: Psest_2283 - Parvulin-like peptidyl-prolyl isomerase, at 2,431,300 to 2,433,156
_2283
Position (kb)
2429
2430
2431 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2428.259 kb on - strand, within Psest_2279 at 2428.261 kb on + strand, within Psest_2279 at 2428.261 kb on + strand, within Psest_2279 at 2428.262 kb on - strand, within Psest_2279 at 2428.262 kb on - strand, within Psest_2279 at 2428.262 kb on - strand, within Psest_2279 at 2428.620 kb on + strand at 2428.621 kb on - strand at 2428.747 kb on - strand at 2428.846 kb on + strand, within Psest_2280 at 2428.846 kb on + strand, within Psest_2280 at 2428.846 kb on + strand, within Psest_2280 at 2428.846 kb on + strand, within Psest_2280 at 2428.847 kb on - strand, within Psest_2280 at 2428.847 kb on - strand, within Psest_2280 at 2428.847 kb on - strand, within Psest_2280 at 2429.040 kb on + strand, within Psest_2280 at 2429.040 kb on + strand, within Psest_2280 at 2429.041 kb on - strand, within Psest_2280 at 2429.041 kb on - strand, within Psest_2280 at 2429.189 kb on - strand, within Psest_2280 at 2429.292 kb on + strand at 2429.292 kb on + strand at 2429.293 kb on - strand at 2429.293 kb on - strand at 2429.458 kb on + strand, within Psest_2281 at 2429.478 kb on + strand, within Psest_2281 at 2429.479 kb on - strand, within Psest_2281 at 2429.479 kb on - strand, within Psest_2281 at 2429.776 kb on + strand, within Psest_2281 at 2429.848 kb on - strand, within Psest_2281 at 2429.848 kb on - strand, within Psest_2281 at 2429.890 kb on + strand, within Psest_2281 at 2429.890 kb on + strand, within Psest_2281 at 2429.891 kb on - strand, within Psest_2281 at 2429.891 kb on - strand, within Psest_2281 at 2429.897 kb on - strand, within Psest_2281 at 2429.953 kb on - strand, within Psest_2281 at 2429.953 kb on - strand, within Psest_2281 at 2430.045 kb on - strand, within Psest_2281 at 2430.126 kb on + strand, within Psest_2281 at 2430.126 kb on + strand, within Psest_2281 at 2430.272 kb on - strand at 2430.272 kb on - strand at 2430.347 kb on + strand at 2430.348 kb on - strand at 2430.357 kb on + strand at 2430.358 kb on - strand at 2430.479 kb on + strand, within Psest_2282 at 2430.479 kb on + strand, within Psest_2282 at 2430.722 kb on - strand, within Psest_2282 at 2430.866 kb on + strand, within Psest_2282 at 2430.939 kb on - strand, within Psest_2282
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 2,428,259 - Psest_2279 0.62 +0.4 2,428,261 + Psest_2279 0.63 -2.4 2,428,261 + Psest_2279 0.63 +0.2 2,428,262 - Psest_2279 0.63 -0.2 2,428,262 - Psest_2279 0.63 -0.3 2,428,262 - Psest_2279 0.63 +0.2 2,428,620 + +1.5 2,428,621 - +0.5 2,428,747 - +0.9 2,428,846 + Psest_2280 0.14 -1.7 2,428,846 + Psest_2280 0.14 -0.7 2,428,846 + Psest_2280 0.14 -2.0 2,428,846 + Psest_2280 0.14 -0.8 2,428,847 - Psest_2280 0.14 -0.1 2,428,847 - Psest_2280 0.14 -0.5 2,428,847 - Psest_2280 0.14 -1.3 2,429,040 + Psest_2280 0.53 +1.6 2,429,040 + Psest_2280 0.53 +0.3 2,429,041 - Psest_2280 0.53 +1.0 2,429,041 - Psest_2280 0.53 +1.6 2,429,189 - Psest_2280 0.82 +0.9 2,429,292 + +0.1 2,429,292 + +0.2 2,429,293 - +1.3 2,429,293 - -1.8 2,429,458 + Psest_2281 0.18 +0.3 2,429,478 + Psest_2281 0.20 +0.9 2,429,479 - Psest_2281 0.20 +2.3 2,429,479 - Psest_2281 0.20 -0.3 2,429,776 + Psest_2281 0.47 -0.1 2,429,848 - Psest_2281 0.53 +2.8 2,429,848 - Psest_2281 0.53 +0.6 2,429,890 + Psest_2281 0.57 +0.4 2,429,890 + Psest_2281 0.57 -1.1 2,429,891 - Psest_2281 0.57 +1.0 2,429,891 - Psest_2281 0.57 -1.7 2,429,897 - Psest_2281 0.58 +1.0 2,429,953 - Psest_2281 0.63 -0.2 2,429,953 - Psest_2281 0.63 -2.8 2,430,045 - Psest_2281 0.71 +0.6 2,430,126 + Psest_2281 0.79 +0.6 2,430,126 + Psest_2281 0.79 +0.6 2,430,272 - +0.1 2,430,272 - +0.7 2,430,347 + -0.5 2,430,348 - -0.2 2,430,357 + +0.2 2,430,358 - -0.0 2,430,479 + Psest_2282 0.15 -0.3 2,430,479 + Psest_2282 0.15 -0.1 2,430,722 - Psest_2282 0.44 +0.4 2,430,866 + Psest_2282 0.61 +1.0 2,430,939 - Psest_2282 0.69 -0.6
Or see this region's nucleotide sequence