Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0258 and Psest_0259 overlap by 4 nucleotides Psest_0259 and Psest_0260 overlap by 4 nucleotides Psest_0260 and Psest_0261 are separated by 112 nucleotides
Psest_0258: Psest_0258 - signal recognition particle-docking protein FtsY, at 272,579 to 273,859
_0258
Psest_0259: Psest_0259 - cell division ATP-binding protein FtsE, at 273,856 to 274,527
_0259
Psest_0260: Psest_0260 - Cell division protein, at 274,524 to 275,543
_0260
Psest_0261: Psest_0261 - alternative sigma factor RpoH, at 275,656 to 276,510
_0261
Position (kb) 274
275
276 Strain fitness (log2 ratio) -2
-1
0
1 at 274.314 kb on - strand, within Psest_0259 at 274.347 kb on + strand, within Psest_0259 at 275.283 kb on + strand, within Psest_0260 at 275.284 kb on - strand, within Psest_0260 at 275.304 kb on + strand, within Psest_0260 at 275.305 kb on - strand, within Psest_0260 at 275.491 kb on + strand at 275.491 kb on + strand at 275.492 kb on - strand at 275.622 kb on + strand at 275.622 kb on + strand at 275.622 kb on + strand at 276.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 274,314 - Psest_0259 0.68 -0.2 274,347 + Psest_0259 0.73 +0.5 275,283 + Psest_0260 0.74 -1.8 275,284 - Psest_0260 0.75 -0.3 275,304 + Psest_0260 0.76 -0.6 275,305 - Psest_0260 0.77 +1.1 275,491 + +1.6 275,491 + -0.2 275,492 - -0.5 275,622 + -0.8 275,622 + +1.2 275,622 + -0.4 276,524 - -0.4
Or see this region's nucleotide sequence