Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3401 and Psest_3402 are separated by 3 nucleotides Psest_3402 and Psest_3403 are separated by 57 nucleotides Psest_3403 and Psest_3404 are separated by 144 nucleotides
Psest_3401: Psest_3401 - DNA repair protein RadA, at 3,568,806 to 3,570,167
_3401
Psest_3402: Psest_3402 - PilZ domain., at 3,570,171 to 3,570,539
_3402
Psest_3403: Psest_3403 - Putative protein-S-isoprenylcysteine methyltransferase, at 3,570,597 to 3,571,058
_3403
Psest_3404: Psest_3404 - Glycine/serine hydroxymethyltransferase, at 3,571,203 to 3,572,456
_3404
Position (kb)
3570
3571
3572 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3569.847 kb on + strand, within Psest_3401 at 3569.847 kb on + strand, within Psest_3401 at 3569.848 kb on - strand, within Psest_3401 at 3569.848 kb on - strand, within Psest_3401 at 3569.848 kb on - strand, within Psest_3401 at 3570.273 kb on - strand, within Psest_3402 at 3570.531 kb on - strand at 3570.569 kb on + strand at 3570.570 kb on - strand at 3570.570 kb on - strand at 3570.571 kb on + strand at 3570.571 kb on + strand at 3570.571 kb on + strand at 3570.571 kb on + strand at 3570.571 kb on + strand at 3570.571 kb on + strand at 3570.572 kb on - strand at 3570.701 kb on - strand, within Psest_3403 at 3570.701 kb on - strand, within Psest_3403 at 3570.701 kb on - strand, within Psest_3403 at 3570.701 kb on - strand, within Psest_3403 at 3570.701 kb on - strand, within Psest_3403 at 3570.763 kb on + strand, within Psest_3403 at 3570.763 kb on + strand, within Psest_3403 at 3570.763 kb on + strand, within Psest_3403 at 3570.763 kb on + strand, within Psest_3403 at 3570.763 kb on + strand, within Psest_3403 at 3570.763 kb on + strand, within Psest_3403 at 3570.764 kb on - strand, within Psest_3403 at 3570.767 kb on + strand, within Psest_3403 at 3570.768 kb on - strand, within Psest_3403 at 3570.768 kb on - strand, within Psest_3403 at 3570.768 kb on - strand, within Psest_3403 at 3570.768 kb on - strand, within Psest_3403 at 3570.784 kb on + strand, within Psest_3403 at 3570.784 kb on + strand, within Psest_3403 at 3570.784 kb on + strand, within Psest_3403 at 3570.784 kb on + strand, within Psest_3403 at 3570.785 kb on - strand, within Psest_3403 at 3570.788 kb on + strand, within Psest_3403 at 3570.788 kb on + strand, within Psest_3403 at 3570.789 kb on - strand, within Psest_3403 at 3570.789 kb on - strand, within Psest_3403 at 3570.789 kb on - strand, within Psest_3403 at 3570.789 kb on - strand, within Psest_3403 at 3570.794 kb on + strand, within Psest_3403 at 3570.794 kb on + strand, within Psest_3403 at 3570.794 kb on + strand, within Psest_3403 at 3570.795 kb on - strand, within Psest_3403 at 3570.795 kb on - strand, within Psest_3403 at 3570.795 kb on - strand, within Psest_3403 at 3570.795 kb on - strand, within Psest_3403 at 3570.934 kb on + strand, within Psest_3403 at 3570.934 kb on + strand, within Psest_3403 at 3570.935 kb on - strand, within Psest_3403 at 3570.935 kb on - strand, within Psest_3403 at 3570.935 kb on - strand, within Psest_3403 at 3570.935 kb on - strand, within Psest_3403 at 3571.012 kb on + strand at 3571.012 kb on + strand at 3571.012 kb on + strand at 3571.012 kb on + strand at 3571.012 kb on + strand at 3571.013 kb on - strand at 3571.013 kb on - strand at 3571.013 kb on - strand at 3571.013 kb on - strand at 3571.013 kb on - strand at 3571.058 kb on + strand at 3571.058 kb on + strand at 3571.059 kb on - strand at 3571.059 kb on - strand at 3571.059 kb on - strand at 3571.059 kb on - strand at 3571.155 kb on + strand at 3571.156 kb on - strand at 3571.204 kb on + strand at 3571.204 kb on + strand at 3571.205 kb on - strand at 3571.205 kb on - strand at 3571.205 kb on - strand at 3571.205 kb on - strand at 3571.205 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 3,569,847 + Psest_3401 0.76 -1.7 3,569,847 + Psest_3401 0.76 +0.1 3,569,848 - Psest_3401 0.77 +2.4 3,569,848 - Psest_3401 0.77 -3.1 3,569,848 - Psest_3401 0.77 +0.5 3,570,273 - Psest_3402 0.28 -0.1 3,570,531 - +0.5 3,570,569 + +0.2 3,570,570 - -0.1 3,570,570 - +0.9 3,570,571 + -0.5 3,570,571 + +0.4 3,570,571 + -0.2 3,570,571 + -0.3 3,570,571 + -2.0 3,570,571 + +1.0 3,570,572 - +1.0 3,570,701 - Psest_3403 0.23 -0.1 3,570,701 - Psest_3403 0.23 -0.7 3,570,701 - Psest_3403 0.23 -1.8 3,570,701 - Psest_3403 0.23 -1.8 3,570,701 - Psest_3403 0.23 -1.7 3,570,763 + Psest_3403 0.36 -0.3 3,570,763 + Psest_3403 0.36 -0.6 3,570,763 + Psest_3403 0.36 +0.1 3,570,763 + Psest_3403 0.36 +0.2 3,570,763 + Psest_3403 0.36 -0.1 3,570,763 + Psest_3403 0.36 -1.0 3,570,764 - Psest_3403 0.36 -0.5 3,570,767 + Psest_3403 0.37 +0.3 3,570,768 - Psest_3403 0.37 +0.1 3,570,768 - Psest_3403 0.37 -1.0 3,570,768 - Psest_3403 0.37 +0.2 3,570,768 - Psest_3403 0.37 -0.3 3,570,784 + Psest_3403 0.40 -0.3 3,570,784 + Psest_3403 0.40 +0.2 3,570,784 + Psest_3403 0.40 +0.1 3,570,784 + Psest_3403 0.40 -0.2 3,570,785 - Psest_3403 0.41 +0.2 3,570,788 + Psest_3403 0.41 -0.5 3,570,788 + Psest_3403 0.41 +1.3 3,570,789 - Psest_3403 0.42 +0.0 3,570,789 - Psest_3403 0.42 +0.1 3,570,789 - Psest_3403 0.42 +0.2 3,570,789 - Psest_3403 0.42 -0.8 3,570,794 + Psest_3403 0.43 -0.7 3,570,794 + Psest_3403 0.43 -0.6 3,570,794 + Psest_3403 0.43 +0.1 3,570,795 - Psest_3403 0.43 +0.7 3,570,795 - Psest_3403 0.43 -1.2 3,570,795 - Psest_3403 0.43 -1.5 3,570,795 - Psest_3403 0.43 +1.0 3,570,934 + Psest_3403 0.73 +1.1 3,570,934 + Psest_3403 0.73 -0.2 3,570,935 - Psest_3403 0.73 +0.0 3,570,935 - Psest_3403 0.73 +0.2 3,570,935 - Psest_3403 0.73 +0.0 3,570,935 - Psest_3403 0.73 -0.4 3,571,012 + -0.8 3,571,012 + -0.5 3,571,012 + +0.2 3,571,012 + +0.4 3,571,012 + +0.7 3,571,013 - +1.2 3,571,013 - -0.6 3,571,013 - -0.8 3,571,013 - -0.4 3,571,013 - -0.5 3,571,058 + +0.2 3,571,058 + +0.3 3,571,059 - +0.1 3,571,059 - -1.2 3,571,059 - -1.7 3,571,059 - +1.9 3,571,155 + +0.2 3,571,156 - -0.3 3,571,204 + +0.7 3,571,204 + -0.3 3,571,205 - +0.3 3,571,205 - -1.7 3,571,205 - -1.3 3,571,205 - -1.0 3,571,205 - -0.4
Or see this region's nucleotide sequence