Experiment: TIme6-nophage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_654 and Pf1N1B4_655 overlap by 4 nucleotides Pf1N1B4_655 and Pf1N1B4_656 are separated by 164 nucleotides Pf1N1B4_656 and Pf1N1B4_657 are separated by 133 nucleotides
Pf1N1B4_654 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein, at 748,397 to 749,341
_654
Pf1N1B4_655 - Glutathione S-transferase (EC 2.5.1.18), at 749,338 to 749,970
_655
Pf1N1B4_656 - Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1), at 750,135 to 751,352
_656
Pf1N1B4_657 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 751,486 to 754,221
_657
Position (kb) 750
751
752 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 749.324 kb on + strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.347 kb on + strand at 749.347 kb on + strand at 749.348 kb on - strand at 749.348 kb on - strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.397 kb on - strand at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.437 kb on + strand, within Pf1N1B4_655 at 749.437 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.459 kb on - strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.750 kb on + strand, within Pf1N1B4_655 at 749.751 kb on - strand, within Pf1N1B4_655 at 749.882 kb on + strand, within Pf1N1B4_655 at 749.989 kb on - strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.145 kb on + strand at 750.166 kb on + strand at 750.166 kb on + strand at 750.167 kb on - strand at 750.167 kb on - strand at 750.167 kb on - strand at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.776 kb on - strand, within Pf1N1B4_656 at 750.842 kb on + strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 751.016 kb on - strand at 751.138 kb on + strand, within Pf1N1B4_656 at 751.139 kb on - strand, within Pf1N1B4_656 at 751.143 kb on + strand, within Pf1N1B4_656 at 751.365 kb on + strand at 751.366 kb on - strand at 751.372 kb on + strand at 751.372 kb on + strand at 751.372 kb on + strand at 751.372 kb on + strand at 751.373 kb on - strand at 751.373 kb on - strand at 751.373 kb on - strand at 751.401 kb on + strand at 751.401 kb on + strand at 751.401 kb on + strand at 751.401 kb on + strand at 751.402 kb on - strand at 751.419 kb on + strand at 751.419 kb on + strand at 751.419 kb on + strand at 751.419 kb on + strand at 751.419 kb on + strand at 751.444 kb on + strand at 751.445 kb on - strand at 751.445 kb on - strand at 751.445 kb on - strand at 751.474 kb on + strand at 751.475 kb on - strand at 751.475 kb on - strand at 751.475 kb on - strand at 751.487 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TIme6-nophage remove 749,324 + +0.3 749,325 - +1.3 749,325 - +1.0 749,325 - -0.7 749,325 - -0.0 749,325 - -1.1 749,325 - +0.1 749,347 + -0.4 749,347 + -1.1 749,348 - +0.0 749,348 - +0.3 749,396 + +0.5 749,396 + +1.6 749,396 + -0.6 749,396 + -0.6 749,396 + -1.0 749,396 + -0.5 749,397 - +1.6 749,411 + Pf1N1B4_655 0.12 +0.5 749,411 + Pf1N1B4_655 0.12 -0.8 749,411 + Pf1N1B4_655 0.12 -0.4 749,411 + Pf1N1B4_655 0.12 +0.3 749,412 - Pf1N1B4_655 0.12 +0.7 749,412 - Pf1N1B4_655 0.12 -0.4 749,412 - Pf1N1B4_655 0.12 +0.3 749,412 - Pf1N1B4_655 0.12 -0.3 749,412 - Pf1N1B4_655 0.12 +0.5 749,437 + Pf1N1B4_655 0.16 +0.7 749,437 + Pf1N1B4_655 0.16 -0.1 749,444 + Pf1N1B4_655 0.17 +1.9 749,444 + Pf1N1B4_655 0.17 -0.4 749,444 + Pf1N1B4_655 0.17 +0.6 749,444 + Pf1N1B4_655 0.17 +0.3 749,445 - Pf1N1B4_655 0.17 +0.2 749,445 - Pf1N1B4_655 0.17 -0.3 749,445 - Pf1N1B4_655 0.17 -0.5 749,445 - Pf1N1B4_655 0.17 -0.3 749,445 - Pf1N1B4_655 0.17 -0.3 749,459 - Pf1N1B4_655 0.19 +1.3 749,634 + Pf1N1B4_655 0.47 -0.3 749,634 + Pf1N1B4_655 0.47 +0.7 749,634 + Pf1N1B4_655 0.47 +0.7 749,635 - Pf1N1B4_655 0.47 -0.3 749,635 - Pf1N1B4_655 0.47 -0.3 749,635 - Pf1N1B4_655 0.47 +0.2 749,635 - Pf1N1B4_655 0.47 +1.9 749,635 - Pf1N1B4_655 0.47 +0.2 749,750 + Pf1N1B4_655 0.65 -0.2 749,751 - Pf1N1B4_655 0.65 -1.7 749,882 + Pf1N1B4_655 0.86 -0.9 749,989 - +1.0 750,136 + -0.3 750,136 + -1.5 750,136 + -0.7 750,136 + -2.3 750,136 + +0.3 750,137 - +0.0 750,137 - +0.7 750,137 - +1.2 750,137 - +0.0 750,137 - -1.3 750,140 + -0.4 750,140 + -1.3 750,140 + -0.3 750,140 + +0.2 750,140 + -1.5 750,140 + -1.4 750,140 + -1.1 750,140 + -0.3 750,140 + -1.3 750,140 + -0.3 750,140 + -2.0 750,140 + -1.8 750,140 + -0.6 750,140 + +0.2 750,140 + -1.1 750,141 - -1.0 750,141 - -1.0 750,141 - -1.9 750,141 - -0.7 750,141 - +0.1 750,141 - -2.3 750,141 - -1.2 750,141 - -1.5 750,141 - -0.1 750,141 - +0.4 750,141 - +0.0 750,141 - -0.9 750,141 - +0.5 750,141 - -1.6 750,141 - -1.4 750,145 + -1.6 750,166 + -1.7 750,166 + -1.1 750,167 - -0.2 750,167 - -0.9 750,167 - -1.3 750,280 + Pf1N1B4_656 0.12 +1.1 750,280 + Pf1N1B4_656 0.12 -2.1 750,280 + Pf1N1B4_656 0.12 -0.4 750,280 + Pf1N1B4_656 0.12 +0.6 750,280 + Pf1N1B4_656 0.12 -0.3 750,280 + Pf1N1B4_656 0.12 +0.8 750,281 - Pf1N1B4_656 0.12 -1.3 750,281 - Pf1N1B4_656 0.12 +0.1 750,281 - Pf1N1B4_656 0.12 -0.2 750,281 - Pf1N1B4_656 0.12 -0.8 750,281 - Pf1N1B4_656 0.12 -1.0 750,281 - Pf1N1B4_656 0.12 -0.1 750,281 - Pf1N1B4_656 0.12 -0.1 750,330 + Pf1N1B4_656 0.16 -1.1 750,330 + -0.3 750,330 + Pf1N1B4_656 0.16 -0.4 750,330 + Pf1N1B4_656 0.16 -2.0 750,330 + Pf1N1B4_656 0.16 -0.8 750,331 - Pf1N1B4_656 0.16 +0.7 750,331 - Pf1N1B4_656 0.16 -0.3 750,331 - Pf1N1B4_656 0.16 -1.6 750,331 - Pf1N1B4_656 0.16 -1.7 750,331 - Pf1N1B4_656 0.16 -1.0 750,775 + Pf1N1B4_656 0.53 -3.0 750,775 + Pf1N1B4_656 0.53 -1.4 750,775 + Pf1N1B4_656 0.53 -0.8 750,775 + Pf1N1B4_656 0.53 -0.4 750,775 + Pf1N1B4_656 0.53 -1.2 750,775 + Pf1N1B4_656 0.53 -0.4 750,776 - Pf1N1B4_656 0.53 -0.3 750,842 + Pf1N1B4_656 0.58 -0.5 750,843 - Pf1N1B4_656 0.58 -0.4 750,843 - Pf1N1B4_656 0.58 +0.6 750,843 - Pf1N1B4_656 0.58 -1.5 750,843 - Pf1N1B4_656 0.58 -0.4 750,843 - Pf1N1B4_656 0.58 -1.5 751,016 - +0.6 751,138 + Pf1N1B4_656 0.82 -1.8 751,139 - Pf1N1B4_656 0.82 -0.7 751,143 + Pf1N1B4_656 0.83 -0.5 751,365 + -2.0 751,366 - +0.7 751,372 + -1.3 751,372 + -1.3 751,372 + -1.5 751,372 + -1.5 751,373 - +0.3 751,373 - -0.5 751,373 - +0.7 751,401 + -1.1 751,401 + -0.1 751,401 + -0.3 751,401 + -1.0 751,402 - -0.5 751,419 + -1.2 751,419 + -1.3 751,419 + +0.1 751,419 + -1.0 751,419 + -1.5 751,444 + -1.1 751,445 - -0.5 751,445 - -0.1 751,445 - +0.9 751,474 + -1.3 751,475 - +1.3 751,475 - +0.1 751,475 - +0.5 751,487 + -1.4
Or see this region's nucleotide sequence