Experiment: TIme6-nophage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_121 and Pf1N1B4_122 overlap by 4 nucleotides Pf1N1B4_122 and Pf1N1B4_123 are separated by 9 nucleotides Pf1N1B4_123 and Pf1N1B4_124 are separated by 245 nucleotides
Pf1N1B4_121 - Membrane fusion component of tripartite multidrug resistance system, at 132,172 to 133,293
_121
Pf1N1B4_122 - Inner membrane component of tripartite multidrug resistance system, at 133,290 to 134,882
_122
Pf1N1B4_123 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 134,892 to 135,332
_123
Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023
_124
Position (kb)
133
134
135 Strain fitness (log2 ratio)
-1
0
1
2 at 132.349 kb on + strand, within Pf1N1B4_121 at 132.349 kb on + strand, within Pf1N1B4_121 at 132.350 kb on - strand, within Pf1N1B4_121 at 132.524 kb on + strand, within Pf1N1B4_121 at 132.641 kb on - strand, within Pf1N1B4_121 at 132.856 kb on + strand, within Pf1N1B4_121 at 132.968 kb on + strand, within Pf1N1B4_121 at 133.429 kb on + strand at 133.833 kb on + strand, within Pf1N1B4_122 at 133.834 kb on - strand, within Pf1N1B4_122 at 133.930 kb on - strand, within Pf1N1B4_122 at 133.975 kb on - strand, within Pf1N1B4_122 at 134.187 kb on + strand, within Pf1N1B4_122 at 134.293 kb on - strand, within Pf1N1B4_122 at 134.675 kb on + strand, within Pf1N1B4_122 at 134.676 kb on - strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.773 kb on - strand at 134.894 kb on - strand at 135.011 kb on - strand, within Pf1N1B4_123 at 135.011 kb on - strand, within Pf1N1B4_123 at 135.311 kb on - strand at 135.389 kb on - strand at 135.389 kb on - strand at 135.394 kb on + strand at 135.403 kb on - strand at 135.403 kb on - strand at 135.425 kb on + strand at 135.526 kb on + strand at 135.735 kb on + strand, within Pf1N1B4_124
Per-strain Table
Position Strand Gene LocusTag Fraction TIme6-nophage remove 132,349 + Pf1N1B4_121 0.16 +0.3 132,349 + Pf1N1B4_121 0.16 -0.0 132,350 - Pf1N1B4_121 0.16 -0.3 132,524 + Pf1N1B4_121 0.31 -1.3 132,641 - Pf1N1B4_121 0.42 -0.4 132,856 + Pf1N1B4_121 0.61 +0.8 132,968 + Pf1N1B4_121 0.71 -0.1 133,429 + -0.2 133,833 + Pf1N1B4_122 0.34 -1.4 133,834 - Pf1N1B4_122 0.34 +1.0 133,930 - Pf1N1B4_122 0.40 -0.6 133,975 - Pf1N1B4_122 0.43 -0.5 134,187 + Pf1N1B4_122 0.56 -0.9 134,293 - Pf1N1B4_122 0.63 -1.6 134,675 + Pf1N1B4_122 0.87 -0.1 134,676 - -0.5 134,772 + -0.1 134,772 + +1.2 134,772 + +0.5 134,773 - +1.5 134,894 - -1.1 135,011 - Pf1N1B4_123 0.27 +0.5 135,011 - Pf1N1B4_123 0.27 -0.7 135,311 - -0.8 135,389 - +0.4 135,389 - +0.3 135,394 + -0.5 135,403 - -0.2 135,403 - -0.1 135,425 + +0.5 135,526 + +2.5 135,735 + Pf1N1B4_124 0.11 -1.1
Or see this region's nucleotide sequence