Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_122

Experiment: TIme6-nophage

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_121 and Pf1N1B4_122 overlap by 4 nucleotidesPf1N1B4_122 and Pf1N1B4_123 are separated by 9 nucleotidesPf1N1B4_123 and Pf1N1B4_124 are separated by 245 nucleotides Pf1N1B4_121 - Membrane fusion component of tripartite multidrug resistance system, at 132,172 to 133,293 _121 Pf1N1B4_122 - Inner membrane component of tripartite multidrug resistance system, at 133,290 to 134,882 _122 Pf1N1B4_123 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 134,892 to 135,332 _123 Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023 _124 Position (kb) 133 134 135Strain fitness (log2 ratio) -1 0 1 2at 132.349 kb on + strand, within Pf1N1B4_121at 132.349 kb on + strand, within Pf1N1B4_121at 132.350 kb on - strand, within Pf1N1B4_121at 132.524 kb on + strand, within Pf1N1B4_121at 132.641 kb on - strand, within Pf1N1B4_121at 132.856 kb on + strand, within Pf1N1B4_121at 132.968 kb on + strand, within Pf1N1B4_121at 133.429 kb on + strandat 133.833 kb on + strand, within Pf1N1B4_122at 133.834 kb on - strand, within Pf1N1B4_122at 133.930 kb on - strand, within Pf1N1B4_122at 133.975 kb on - strand, within Pf1N1B4_122at 134.187 kb on + strand, within Pf1N1B4_122at 134.293 kb on - strand, within Pf1N1B4_122at 134.675 kb on + strand, within Pf1N1B4_122at 134.676 kb on - strandat 134.772 kb on + strandat 134.772 kb on + strandat 134.772 kb on + strandat 134.773 kb on - strandat 134.894 kb on - strandat 135.011 kb on - strand, within Pf1N1B4_123at 135.011 kb on - strand, within Pf1N1B4_123at 135.311 kb on - strandat 135.389 kb on - strandat 135.389 kb on - strandat 135.394 kb on + strandat 135.403 kb on - strandat 135.403 kb on - strandat 135.425 kb on + strandat 135.526 kb on + strandat 135.735 kb on + strand, within Pf1N1B4_124

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Per-strain Table

Position Strand Gene LocusTag Fraction TIme6-nophage
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132,349 + Pf1N1B4_121 0.16 +0.3
132,349 + Pf1N1B4_121 0.16 -0.0
132,350 - Pf1N1B4_121 0.16 -0.3
132,524 + Pf1N1B4_121 0.31 -1.3
132,641 - Pf1N1B4_121 0.42 -0.4
132,856 + Pf1N1B4_121 0.61 +0.8
132,968 + Pf1N1B4_121 0.71 -0.1
133,429 + -0.2
133,833 + Pf1N1B4_122 0.34 -1.4
133,834 - Pf1N1B4_122 0.34 +1.0
133,930 - Pf1N1B4_122 0.40 -0.6
133,975 - Pf1N1B4_122 0.43 -0.5
134,187 + Pf1N1B4_122 0.56 -0.9
134,293 - Pf1N1B4_122 0.63 -1.6
134,675 + Pf1N1B4_122 0.87 -0.1
134,676 - -0.5
134,772 + -0.1
134,772 + +1.2
134,772 + +0.5
134,773 - +1.5
134,894 - -1.1
135,011 - Pf1N1B4_123 0.27 +0.5
135,011 - Pf1N1B4_123 0.27 -0.7
135,311 - -0.8
135,389 - +0.4
135,389 - +0.3
135,394 + -0.5
135,403 - -0.2
135,403 - -0.1
135,425 + +0.5
135,526 + +2.5
135,735 + Pf1N1B4_124 0.11 -1.1

Or see this region's nucleotide sequence