Experiment: Time16-nophage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2022 and Pf1N1B4_2023 are separated by 173 nucleotides Pf1N1B4_2023 and Pf1N1B4_2024 are separated by 175 nucleotides
Pf1N1B4_2022 - Transcriptional regulator, AraC family, at 2,307,473 to 2,308,471
_2022
Pf1N1B4_2023 - L-asparaginase (EC 3.5.1.1) (from data) , at 2,308,645 to 2,309,649
_2023
Pf1N1B4_2024 - sodium/alanine transporter, at 2,309,825 to 2,311,276
_2024
Position (kb)
2308
2309
2310 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2308.158 kb on - strand at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.214 kb on + strand, within Pf1N1B4_2022 at 2308.215 kb on - strand, within Pf1N1B4_2022 at 2308.215 kb on - strand, within Pf1N1B4_2022 at 2308.237 kb on - strand, within Pf1N1B4_2022 at 2308.286 kb on + strand at 2308.286 kb on + strand, within Pf1N1B4_2022 at 2308.287 kb on - strand, within Pf1N1B4_2022 at 2308.488 kb on + strand at 2308.488 kb on + strand at 2308.488 kb on + strand at 2308.489 kb on - strand at 2308.489 kb on - strand at 2308.492 kb on + strand at 2308.511 kb on + strand at 2308.511 kb on + strand at 2308.511 kb on + strand at 2308.511 kb on + strand at 2308.511 kb on + strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.513 kb on + strand at 2308.513 kb on + strand at 2308.513 kb on + strand at 2308.584 kb on + strand at 2308.585 kb on - strand at 2308.585 kb on - strand at 2308.718 kb on - strand at 2308.805 kb on + strand, within Pf1N1B4_2023 at 2308.805 kb on + strand, within Pf1N1B4_2023 at 2308.931 kb on + strand, within Pf1N1B4_2023 at 2308.931 kb on + strand, within Pf1N1B4_2023 at 2309.037 kb on + strand, within Pf1N1B4_2023 at 2309.038 kb on - strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.339 kb on + strand, within Pf1N1B4_2023 at 2309.340 kb on - strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.523 kb on - strand, within Pf1N1B4_2023 at 2309.523 kb on - strand, within Pf1N1B4_2023 at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.753 kb on + strand at 2309.754 kb on - strand at 2310.126 kb on - strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.199 kb on - strand at 2310.199 kb on - strand, within Pf1N1B4_2024 at 2310.199 kb on - strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.207 kb on + strand, within Pf1N1B4_2024 at 2310.207 kb on + strand, within Pf1N1B4_2024 at 2310.208 kb on - strand, within Pf1N1B4_2024 at 2310.333 kb on + strand, within Pf1N1B4_2024 at 2310.333 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024
Per-strain Table
Position Strand Gene LocusTag Fraction Time16-nophage remove 2,307,649 - Pf1N1B4_2022 0.18 +0.1 2,307,649 - Pf1N1B4_2022 0.18 -1.0 2,307,649 - Pf1N1B4_2022 0.18 +1.3 2,307,755 + Pf1N1B4_2022 0.28 -1.7 2,307,755 + Pf1N1B4_2022 0.28 +0.4 2,307,755 + Pf1N1B4_2022 0.28 -0.8 2,307,756 - Pf1N1B4_2022 0.28 -0.4 2,307,756 - Pf1N1B4_2022 0.28 -0.5 2,307,756 - Pf1N1B4_2022 0.28 +0.1 2,307,756 - Pf1N1B4_2022 0.28 +0.5 2,308,158 - +0.5 2,308,196 + Pf1N1B4_2022 0.72 +0.3 2,308,196 + Pf1N1B4_2022 0.72 -0.7 2,308,196 + Pf1N1B4_2022 0.72 -0.4 2,308,196 + Pf1N1B4_2022 0.72 +0.3 2,308,197 - Pf1N1B4_2022 0.72 -0.9 2,308,197 - Pf1N1B4_2022 0.72 -1.0 2,308,197 - Pf1N1B4_2022 0.72 +0.3 2,308,214 + Pf1N1B4_2022 0.74 -0.8 2,308,215 - Pf1N1B4_2022 0.74 -0.4 2,308,215 - Pf1N1B4_2022 0.74 -0.8 2,308,237 - Pf1N1B4_2022 0.76 -0.1 2,308,286 + -0.1 2,308,286 + Pf1N1B4_2022 0.81 -0.7 2,308,287 - Pf1N1B4_2022 0.81 +0.5 2,308,488 + -0.7 2,308,488 + +0.2 2,308,488 + +0.3 2,308,489 - -1.1 2,308,489 - +0.1 2,308,492 + -1.7 2,308,511 + -1.1 2,308,511 + +0.1 2,308,511 + -1.3 2,308,511 + +0.5 2,308,511 + -1.4 2,308,512 - -0.6 2,308,512 - -0.1 2,308,512 - -1.0 2,308,512 - -0.5 2,308,512 - +0.2 2,308,512 - +1.1 2,308,512 - +0.5 2,308,512 - -0.8 2,308,513 + -0.7 2,308,513 + +0.7 2,308,513 + -1.3 2,308,584 + +0.5 2,308,585 - -0.9 2,308,585 - -0.4 2,308,718 - -1.9 2,308,805 + Pf1N1B4_2023 0.16 +0.0 2,308,805 + Pf1N1B4_2023 0.16 -0.6 2,308,931 + Pf1N1B4_2023 0.28 -0.9 2,308,931 + Pf1N1B4_2023 0.28 -0.9 2,309,037 + Pf1N1B4_2023 0.39 -0.3 2,309,038 - Pf1N1B4_2023 0.39 -1.5 2,309,048 + Pf1N1B4_2023 0.40 -1.2 2,309,048 + Pf1N1B4_2023 0.40 +0.1 2,309,048 + Pf1N1B4_2023 0.40 -1.8 2,309,049 - Pf1N1B4_2023 0.40 -1.6 2,309,049 - Pf1N1B4_2023 0.40 -1.7 2,309,049 - Pf1N1B4_2023 0.40 -1.3 2,309,049 - Pf1N1B4_2023 0.40 -0.8 2,309,177 + Pf1N1B4_2023 0.53 -1.1 2,309,177 + Pf1N1B4_2023 0.53 +1.4 2,309,177 + Pf1N1B4_2023 0.53 -1.4 2,309,178 - Pf1N1B4_2023 0.53 -2.2 2,309,178 - Pf1N1B4_2023 0.53 -1.1 2,309,178 - Pf1N1B4_2023 0.53 -1.3 2,309,178 - Pf1N1B4_2023 0.53 -0.3 2,309,178 - Pf1N1B4_2023 0.53 -0.9 2,309,178 - Pf1N1B4_2023 0.53 -0.1 2,309,339 + Pf1N1B4_2023 0.69 -0.9 2,309,340 - Pf1N1B4_2023 0.69 -0.7 2,309,522 + Pf1N1B4_2023 0.87 -1.8 2,309,522 + Pf1N1B4_2023 0.87 +0.2 2,309,522 + Pf1N1B4_2023 0.87 -1.5 2,309,523 - Pf1N1B4_2023 0.87 -1.1 2,309,523 - Pf1N1B4_2023 0.87 -1.2 2,309,604 - -1.0 2,309,604 - +0.5 2,309,604 - -1.3 2,309,604 - -0.8 2,309,604 - -0.9 2,309,604 - -1.3 2,309,753 + -1.3 2,309,754 - +2.7 2,310,126 - Pf1N1B4_2024 0.21 -1.1 2,310,198 + Pf1N1B4_2024 0.26 +0.3 2,310,198 + Pf1N1B4_2024 0.26 -0.1 2,310,198 + Pf1N1B4_2024 0.26 -0.1 2,310,199 - -0.5 2,310,199 - Pf1N1B4_2024 0.26 +1.7 2,310,199 - Pf1N1B4_2024 0.26 -0.2 2,310,201 + Pf1N1B4_2024 0.26 +0.2 2,310,201 + Pf1N1B4_2024 0.26 +0.7 2,310,201 + Pf1N1B4_2024 0.26 +0.1 2,310,201 + Pf1N1B4_2024 0.26 -0.7 2,310,201 + Pf1N1B4_2024 0.26 +0.4 2,310,201 + Pf1N1B4_2024 0.26 -1.8 2,310,202 - Pf1N1B4_2024 0.26 +1.7 2,310,202 - Pf1N1B4_2024 0.26 +1.4 2,310,202 - Pf1N1B4_2024 0.26 +0.1 2,310,202 - Pf1N1B4_2024 0.26 -1.5 2,310,202 - Pf1N1B4_2024 0.26 -1.0 2,310,202 - Pf1N1B4_2024 0.26 +0.3 2,310,202 - Pf1N1B4_2024 0.26 +0.0 2,310,202 - Pf1N1B4_2024 0.26 -0.3 2,310,202 - Pf1N1B4_2024 0.26 +0.4 2,310,207 + Pf1N1B4_2024 0.26 -1.0 2,310,207 + Pf1N1B4_2024 0.26 -0.0 2,310,208 - Pf1N1B4_2024 0.26 -2.8 2,310,333 + Pf1N1B4_2024 0.35 -0.8 2,310,333 + Pf1N1B4_2024 0.35 +0.1 2,310,624 + Pf1N1B4_2024 0.55 -0.8 2,310,624 + Pf1N1B4_2024 0.55 -1.1 2,310,624 + Pf1N1B4_2024 0.55 +1.7 2,310,624 + Pf1N1B4_2024 0.55 -0.8 2,310,624 + Pf1N1B4_2024 0.55 +1.4 2,310,625 - Pf1N1B4_2024 0.55 -0.3 2,310,625 - Pf1N1B4_2024 0.55 +0.4 2,310,625 - Pf1N1B4_2024 0.55 -0.2 2,310,625 - Pf1N1B4_2024 0.55 -1.3 2,310,625 - Pf1N1B4_2024 0.55 -0.7 2,310,625 - Pf1N1B4_2024 0.55 -1.7 2,310,625 - Pf1N1B4_2024 0.55 +0.2 2,310,642 + Pf1N1B4_2024 0.56 +0.9 2,310,642 + Pf1N1B4_2024 0.56 -1.2 2,310,642 + Pf1N1B4_2024 0.56 +1.3 2,310,642 + Pf1N1B4_2024 0.56 +0.1 2,310,642 + Pf1N1B4_2024 0.56 -0.6 2,310,642 + Pf1N1B4_2024 0.56 +1.7 2,310,643 - Pf1N1B4_2024 0.56 -1.3 2,310,643 - Pf1N1B4_2024 0.56 +0.6 2,310,643 - Pf1N1B4_2024 0.56 -0.3 2,310,643 - Pf1N1B4_2024 0.56 -0.8
Or see this region's nucleotide sequence