Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5268

Experiment: Time6-FRS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5267 and Pf1N1B4_5268 are separated by 208 nucleotidesPf1N1B4_5268 and Pf1N1B4_5269 are separated by 330 nucleotides Pf1N1B4_5267 - hypothetical protein, at 24,892 to 25,410 _5267 Pf1N1B4_5268 - hypothetical protein, at 25,619 to 26,140 _5268 Pf1N1B4_5269 - hypothetical protein, at 26,471 to 26,764 _5269 Position (kb) 25 26 27Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 24.725 kb on - strandat 24.727 kb on + strandat 24.728 kb on - strandat 24.800 kb on + strandat 24.811 kb on + strandat 24.811 kb on + strandat 24.811 kb on + strandat 24.812 kb on - strandat 25.039 kb on + strand, within Pf1N1B4_5267at 25.040 kb on - strandat 25.040 kb on - strand, within Pf1N1B4_5267at 25.040 kb on - strand, within Pf1N1B4_5267at 25.125 kb on + strand, within Pf1N1B4_5267at 25.125 kb on + strand, within Pf1N1B4_5267at 25.125 kb on + strand, within Pf1N1B4_5267at 25.126 kb on - strand, within Pf1N1B4_5267at 25.126 kb on - strand, within Pf1N1B4_5267at 25.126 kb on - strand, within Pf1N1B4_5267at 25.126 kb on - strand, within Pf1N1B4_5267at 25.204 kb on + strand, within Pf1N1B4_5267at 25.204 kb on + strand, within Pf1N1B4_5267at 25.205 kb on - strand, within Pf1N1B4_5267at 25.320 kb on + strand, within Pf1N1B4_5267at 25.321 kb on - strand, within Pf1N1B4_5267at 25.476 kb on + strandat 25.575 kb on + strandat 25.841 kb on + strand, within Pf1N1B4_5268at 25.850 kb on + strand, within Pf1N1B4_5268at 25.850 kb on + strand, within Pf1N1B4_5268at 25.850 kb on + strand, within Pf1N1B4_5268at 25.850 kb on + strand, within Pf1N1B4_5268at 25.979 kb on + strand, within Pf1N1B4_5268at 25.979 kb on + strand, within Pf1N1B4_5268at 25.979 kb on + strand, within Pf1N1B4_5268at 25.989 kb on + strand, within Pf1N1B4_5268at 25.990 kb on - strand, within Pf1N1B4_5268at 25.990 kb on - strand, within Pf1N1B4_5268at 26.054 kb on + strand, within Pf1N1B4_5268at 26.054 kb on + strand, within Pf1N1B4_5268at 26.054 kb on + strand, within Pf1N1B4_5268at 26.055 kb on - strand, within Pf1N1B4_5268at 26.058 kb on + strandat 26.058 kb on + strand, within Pf1N1B4_5268at 26.058 kb on + strand, within Pf1N1B4_5268at 26.058 kb on + strand, within Pf1N1B4_5268at 26.058 kb on + strand, within Pf1N1B4_5268at 26.058 kb on + strand, within Pf1N1B4_5268at 26.059 kb on - strand, within Pf1N1B4_5268at 26.059 kb on - strand, within Pf1N1B4_5268at 26.128 kb on - strandat 26.128 kb on - strandat 26.166 kb on + strandat 26.166 kb on + strandat 26.167 kb on - strandat 26.189 kb on + strandat 26.189 kb on + strandat 26.190 kb on - strandat 26.190 kb on - strandat 26.227 kb on - strandat 26.348 kb on + strandat 26.348 kb on + strandat 26.382 kb on - strandat 26.385 kb on + strandat 26.386 kb on - strandat 26.521 kb on + strand, within Pf1N1B4_5269at 26.521 kb on + strand, within Pf1N1B4_5269at 26.521 kb on + strand, within Pf1N1B4_5269at 26.522 kb on - strand, within Pf1N1B4_5269at 26.660 kb on + strand, within Pf1N1B4_5269at 26.660 kb on + strand, within Pf1N1B4_5269at 26.660 kb on + strand, within Pf1N1B4_5269at 26.661 kb on - strand, within Pf1N1B4_5269at 26.837 kb on - strandat 26.980 kb on - strandat 26.980 kb on - strandat 26.980 kb on - strandat 27.045 kb on + strandat 27.046 kb on - strandat 27.046 kb on - strandat 27.046 kb on - strandat 27.046 kb on - strandat 27.046 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Time6-FRS
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24,725 - +0.5
24,727 + +1.2
24,728 - -1.8
24,800 + -0.5
24,811 + -0.1
24,811 + -0.2
24,811 + -1.8
24,812 - -0.1
25,039 + Pf1N1B4_5267 0.28 -1.1
25,040 - +1.5
25,040 - Pf1N1B4_5267 0.29 +1.2
25,040 - Pf1N1B4_5267 0.29 -1.3
25,125 + Pf1N1B4_5267 0.45 -1.6
25,125 + Pf1N1B4_5267 0.45 +0.7
25,125 + Pf1N1B4_5267 0.45 -0.7
25,126 - Pf1N1B4_5267 0.45 -2.9
25,126 - Pf1N1B4_5267 0.45 -0.7
25,126 - Pf1N1B4_5267 0.45 -0.1
25,126 - Pf1N1B4_5267 0.45 -1.0
25,204 + Pf1N1B4_5267 0.60 -0.1
25,204 + Pf1N1B4_5267 0.60 +1.2
25,205 - Pf1N1B4_5267 0.60 +0.8
25,320 + Pf1N1B4_5267 0.82 +1.1
25,321 - Pf1N1B4_5267 0.83 -1.7
25,476 + +1.1
25,575 + -0.0
25,841 + Pf1N1B4_5268 0.43 -1.2
25,850 + Pf1N1B4_5268 0.44 +0.8
25,850 + Pf1N1B4_5268 0.44 -0.8
25,850 + Pf1N1B4_5268 0.44 -0.8
25,850 + Pf1N1B4_5268 0.44 -0.1
25,979 + Pf1N1B4_5268 0.69 -0.6
25,979 + Pf1N1B4_5268 0.69 -0.1
25,979 + Pf1N1B4_5268 0.69 +0.5
25,989 + Pf1N1B4_5268 0.71 -1.0
25,990 - Pf1N1B4_5268 0.71 -0.5
25,990 - Pf1N1B4_5268 0.71 +0.2
26,054 + Pf1N1B4_5268 0.83 -0.3
26,054 + Pf1N1B4_5268 0.83 +0.2
26,054 + Pf1N1B4_5268 0.83 -0.7
26,055 - Pf1N1B4_5268 0.84 -0.5
26,058 + -0.1
26,058 + Pf1N1B4_5268 0.84 -1.2
26,058 + Pf1N1B4_5268 0.84 +1.5
26,058 + Pf1N1B4_5268 0.84 -0.5
26,058 + Pf1N1B4_5268 0.84 +1.5
26,058 + Pf1N1B4_5268 0.84 +0.0
26,059 - Pf1N1B4_5268 0.84 +1.0
26,059 - Pf1N1B4_5268 0.84 -0.1
26,128 - -0.0
26,128 - -0.4
26,166 + +2.5
26,166 + +0.7
26,167 - -1.8
26,189 + +0.9
26,189 + +0.0
26,190 - -0.3
26,190 - -0.8
26,227 - +1.1
26,348 + +0.2
26,348 + +0.6
26,382 - +0.1
26,385 + +0.9
26,386 - -0.1
26,521 + Pf1N1B4_5269 0.17 +0.7
26,521 + Pf1N1B4_5269 0.17 +0.3
26,521 + Pf1N1B4_5269 0.17 -0.5
26,522 - Pf1N1B4_5269 0.17 +0.3
26,660 + Pf1N1B4_5269 0.64 -0.3
26,660 + Pf1N1B4_5269 0.64 +0.1
26,660 + Pf1N1B4_5269 0.64 +0.8
26,661 - Pf1N1B4_5269 0.65 -0.8
26,837 - -0.5
26,980 - +0.9
26,980 - +1.2
26,980 - +0.4
27,045 + -1.1
27,046 - -1.8
27,046 - -0.5
27,046 - -0.7
27,046 - +0.9
27,046 - -0.7

Or see this region's nucleotide sequence