Experiment: Time6-FRS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4723 and Pf1N1B4_4724 are separated by 198 nucleotides Pf1N1B4_4724 and Pf1N1B4_4725 are separated by 272 nucleotides Pf1N1B4_4725 and Pf1N1B4_4726 overlap by 4 nucleotides
Pf1N1B4_4723 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 5,218,782 to 5,220,527
_4723
Pf1N1B4_4724 - FIG00961385: hypothetical protein, at 5,220,726 to 5,220,908
_4724
Pf1N1B4_4725 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 5,221,181 to 5,222,161
_4725
Pf1N1B4_4726 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 5,222,158 to 5,223,681
_4726
Position (kb)
5221
5222
5223 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5220.405 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.637 kb on - strand at 5220.637 kb on - strand at 5220.637 kb on - strand at 5220.898 kb on - strand at 5220.940 kb on - strand at 5220.970 kb on - strand at 5221.137 kb on + strand at 5221.137 kb on + strand at 5221.137 kb on + strand at 5221.157 kb on - strand at 5221.162 kb on + strand at 5221.166 kb on - strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.179 kb on - strand at 5221.179 kb on - strand at 5221.222 kb on + strand at 5221.252 kb on + strand at 5221.253 kb on - strand at 5221.265 kb on + strand at 5221.423 kb on + strand, within Pf1N1B4_4725 at 5221.423 kb on + strand, within Pf1N1B4_4725 at 5221.424 kb on - strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.473 kb on - strand at 5221.473 kb on - strand, within Pf1N1B4_4725 at 5221.475 kb on - strand, within Pf1N1B4_4725 at 5221.572 kb on - strand, within Pf1N1B4_4725 at 5222.269 kb on + strand at 5222.269 kb on + strand at 5222.270 kb on - strand at 5222.270 kb on - strand at 5222.270 kb on - strand at 5222.462 kb on - strand, within Pf1N1B4_4726 at 5222.462 kb on - strand, within Pf1N1B4_4726 at 5222.548 kb on + strand, within Pf1N1B4_4726 at 5222.746 kb on + strand, within Pf1N1B4_4726 at 5222.747 kb on - strand, within Pf1N1B4_4726 at 5222.943 kb on - strand, within Pf1N1B4_4726 at 5223.160 kb on + strand, within Pf1N1B4_4726 at 5223.160 kb on + strand, within Pf1N1B4_4726 at 5223.161 kb on - strand, within Pf1N1B4_4726
Per-strain Table
Position Strand Gene LocusTag Fraction Time6-FRS remove 5,220,405 - -0.9 5,220,550 - -0.2 5,220,550 - -0.1 5,220,550 - -0.9 5,220,550 - -0.9 5,220,550 - -0.1 5,220,550 - -0.8 5,220,637 - -0.2 5,220,637 - +0.0 5,220,637 - -0.5 5,220,898 - +0.7 5,220,940 - -1.5 5,220,970 - -0.8 5,221,137 + +1.2 5,221,137 + -0.6 5,221,137 + +2.2 5,221,157 - +0.7 5,221,162 + -1.1 5,221,166 - -1.0 5,221,178 + -0.9 5,221,178 + -0.2 5,221,178 + +0.7 5,221,178 + +1.1 5,221,179 - -0.6 5,221,179 - -2.1 5,221,222 + -0.6 5,221,252 + +0.2 5,221,253 - +1.5 5,221,265 + -0.7 5,221,423 + Pf1N1B4_4725 0.25 +0.2 5,221,423 + Pf1N1B4_4725 0.25 -1.1 5,221,424 - Pf1N1B4_4725 0.25 -0.6 5,221,472 + Pf1N1B4_4725 0.30 -1.0 5,221,472 + Pf1N1B4_4725 0.30 -0.3 5,221,472 + Pf1N1B4_4725 0.30 +0.5 5,221,473 - -1.1 5,221,473 - Pf1N1B4_4725 0.30 +0.2 5,221,475 - Pf1N1B4_4725 0.30 +0.0 5,221,572 - Pf1N1B4_4725 0.40 -0.7 5,222,269 + +0.2 5,222,269 + -2.2 5,222,270 - -0.6 5,222,270 - +1.2 5,222,270 - -0.6 5,222,462 - Pf1N1B4_4726 0.20 -1.6 5,222,462 - Pf1N1B4_4726 0.20 -0.2 5,222,548 + Pf1N1B4_4726 0.26 +0.2 5,222,746 + Pf1N1B4_4726 0.39 -1.8 5,222,747 - Pf1N1B4_4726 0.39 +0.1 5,222,943 - Pf1N1B4_4726 0.52 +1.0 5,223,160 + Pf1N1B4_4726 0.66 -0.1 5,223,160 + Pf1N1B4_4726 0.66 +1.2 5,223,161 - Pf1N1B4_4726 0.66 +0.1
Or see this region's nucleotide sequence