Experiment: Time6-FRS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3582 and Pf1N1B4_3583 are separated by 16 nucleotides Pf1N1B4_3583 and Pf1N1B4_3584 are separated by 51 nucleotides Pf1N1B4_3584 and Pf1N1B4_3585 are separated by 30 nucleotides
Pf1N1B4_3582 - C-methyltransferase, at 3,992,603 to 3,993,673
_3582
Pf1N1B4_3583 - Cephalosporin hydroxylase, at 3,993,690 to 3,994,427
_3583
Pf1N1B4_3584 - O-antigen biosynthesis protein, at 3,994,479 to 3,997,415
_3584
Pf1N1B4_3585 - UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.-), at 3,997,446 to 3,998,447
_3585
Position (kb)
3994
3995
3996
3997
3998 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3993.599 kb on + strand at 3993.599 kb on + strand at 3993.600 kb on - strand at 3993.635 kb on + strand at 3993.636 kb on - strand at 3993.687 kb on - strand at 3993.817 kb on - strand at 3993.817 kb on - strand, within Pf1N1B4_3583 at 3993.817 kb on - strand, within Pf1N1B4_3583 at 3993.823 kb on - strand at 3993.957 kb on + strand, within Pf1N1B4_3583 at 3994.064 kb on + strand, within Pf1N1B4_3583 at 3994.065 kb on - strand, within Pf1N1B4_3583 at 3994.065 kb on - strand, within Pf1N1B4_3583 at 3994.200 kb on + strand, within Pf1N1B4_3583 at 3994.228 kb on - strand, within Pf1N1B4_3583 at 3994.228 kb on - strand, within Pf1N1B4_3583 at 3994.329 kb on + strand, within Pf1N1B4_3583 at 3994.330 kb on - strand, within Pf1N1B4_3583 at 3994.588 kb on - strand at 3994.590 kb on + strand at 3994.590 kb on + strand at 3994.591 kb on - strand at 3994.591 kb on - strand at 3994.591 kb on - strand at 3994.599 kb on + strand at 3994.600 kb on - strand at 3994.701 kb on + strand at 3994.702 kb on - strand at 3994.702 kb on - strand at 3994.755 kb on + strand at 3994.755 kb on + strand at 3994.755 kb on + strand at 3994.755 kb on + strand at 3994.839 kb on + strand, within Pf1N1B4_3584 at 3994.844 kb on + strand, within Pf1N1B4_3584 at 3994.844 kb on + strand, within Pf1N1B4_3584 at 3994.844 kb on + strand, within Pf1N1B4_3584 at 3994.844 kb on + strand, within Pf1N1B4_3584 at 3994.845 kb on - strand, within Pf1N1B4_3584 at 3994.845 kb on - strand, within Pf1N1B4_3584 at 3994.869 kb on + strand, within Pf1N1B4_3584 at 3994.869 kb on + strand, within Pf1N1B4_3584 at 3994.870 kb on - strand, within Pf1N1B4_3584 at 3994.870 kb on - strand, within Pf1N1B4_3584 at 3994.887 kb on + strand, within Pf1N1B4_3584 at 3994.887 kb on + strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.896 kb on + strand, within Pf1N1B4_3584 at 3994.897 kb on - strand at 3994.899 kb on + strand, within Pf1N1B4_3584 at 3994.918 kb on + strand, within Pf1N1B4_3584 at 3994.965 kb on + strand at 3994.966 kb on - strand, within Pf1N1B4_3584 at 3994.977 kb on + strand, within Pf1N1B4_3584 at 3994.977 kb on + strand, within Pf1N1B4_3584 at 3994.978 kb on - strand, within Pf1N1B4_3584 at 3994.978 kb on - strand, within Pf1N1B4_3584 at 3994.978 kb on - strand at 3994.978 kb on - strand, within Pf1N1B4_3584 at 3994.992 kb on + strand, within Pf1N1B4_3584 at 3994.993 kb on - strand, within Pf1N1B4_3584 at 3995.028 kb on + strand, within Pf1N1B4_3584 at 3995.082 kb on + strand, within Pf1N1B4_3584 at 3995.083 kb on - strand, within Pf1N1B4_3584 at 3995.083 kb on - strand, within Pf1N1B4_3584 at 3995.083 kb on - strand, within Pf1N1B4_3584 at 3995.085 kb on + strand, within Pf1N1B4_3584 at 3995.085 kb on + strand, within Pf1N1B4_3584 at 3995.085 kb on + strand, within Pf1N1B4_3584 at 3995.086 kb on - strand, within Pf1N1B4_3584 at 3995.086 kb on - strand, within Pf1N1B4_3584 at 3995.086 kb on - strand, within Pf1N1B4_3584 at 3995.127 kb on + strand, within Pf1N1B4_3584 at 3995.127 kb on + strand, within Pf1N1B4_3584 at 3995.127 kb on + strand, within Pf1N1B4_3584 at 3995.127 kb on + strand, within Pf1N1B4_3584 at 3995.128 kb on - strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.428 kb on - strand, within Pf1N1B4_3584 at 3995.428 kb on - strand at 3995.428 kb on - strand, within Pf1N1B4_3584 at 3995.428 kb on - strand, within Pf1N1B4_3584 at 3995.428 kb on - strand, within Pf1N1B4_3584 at 3995.445 kb on + strand, within Pf1N1B4_3584 at 3995.555 kb on + strand, within Pf1N1B4_3584 at 3995.555 kb on + strand, within Pf1N1B4_3584 at 3995.555 kb on + strand at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.572 kb on - strand, within Pf1N1B4_3584 at 3996.054 kb on + strand, within Pf1N1B4_3584 at 3996.054 kb on + strand at 3996.054 kb on + strand at 3996.054 kb on + strand, within Pf1N1B4_3584 at 3996.843 kb on + strand, within Pf1N1B4_3584 at 3996.844 kb on - strand, within Pf1N1B4_3584 at 3997.051 kb on - strand, within Pf1N1B4_3584 at 3997.170 kb on + strand at 3997.332 kb on + strand at 3997.367 kb on + strand at 3997.414 kb on - strand at 3997.431 kb on - strand at 3997.431 kb on - strand at 3997.575 kb on + strand at 3997.615 kb on - strand, within Pf1N1B4_3585 at 3997.719 kb on + strand, within Pf1N1B4_3585 at 3997.719 kb on + strand, within Pf1N1B4_3585 at 3997.720 kb on - strand, within Pf1N1B4_3585 at 3998.274 kb on + strand, within Pf1N1B4_3585 at 3998.303 kb on + strand, within Pf1N1B4_3585 at 3998.303 kb on + strand, within Pf1N1B4_3585
Per-strain Table
Position Strand Gene LocusTag Fraction Time6-FRS remove 3,993,599 + +0.3 3,993,599 + +0.1 3,993,600 - -1.3 3,993,635 + -0.3 3,993,636 - -0.4 3,993,687 - +0.5 3,993,817 - +1.3 3,993,817 - Pf1N1B4_3583 0.17 -0.7 3,993,817 - Pf1N1B4_3583 0.17 -0.7 3,993,823 - -1.0 3,993,957 + Pf1N1B4_3583 0.36 +0.3 3,994,064 + Pf1N1B4_3583 0.51 +0.3 3,994,065 - Pf1N1B4_3583 0.51 -0.8 3,994,065 - Pf1N1B4_3583 0.51 -1.5 3,994,200 + Pf1N1B4_3583 0.69 -1.9 3,994,228 - Pf1N1B4_3583 0.73 -0.0 3,994,228 - Pf1N1B4_3583 0.73 -0.7 3,994,329 + Pf1N1B4_3583 0.87 -0.2 3,994,330 - Pf1N1B4_3583 0.87 -1.1 3,994,588 - -1.2 3,994,590 + +0.1 3,994,590 + -0.7 3,994,591 - -1.3 3,994,591 - -1.1 3,994,591 - +0.3 3,994,599 + +0.3 3,994,600 - -1.7 3,994,701 + +1.1 3,994,702 - +1.1 3,994,702 - +0.3 3,994,755 + +1.5 3,994,755 + -2.3 3,994,755 + -0.3 3,994,755 + +0.2 3,994,839 + Pf1N1B4_3584 0.12 -0.5 3,994,844 + Pf1N1B4_3584 0.12 -0.7 3,994,844 + Pf1N1B4_3584 0.12 -1.6 3,994,844 + Pf1N1B4_3584 0.12 +0.1 3,994,844 + Pf1N1B4_3584 0.12 +0.4 3,994,845 - Pf1N1B4_3584 0.12 +0.9 3,994,845 - Pf1N1B4_3584 0.12 -0.6 3,994,869 + Pf1N1B4_3584 0.13 +1.3 3,994,869 + Pf1N1B4_3584 0.13 -0.8 3,994,870 - Pf1N1B4_3584 0.13 -0.2 3,994,870 - Pf1N1B4_3584 0.13 -0.9 3,994,887 + Pf1N1B4_3584 0.14 -0.6 3,994,887 + Pf1N1B4_3584 0.14 -2.0 3,994,888 - Pf1N1B4_3584 0.14 +0.5 3,994,888 - Pf1N1B4_3584 0.14 +0.2 3,994,888 - Pf1N1B4_3584 0.14 +0.3 3,994,888 - Pf1N1B4_3584 0.14 -0.2 3,994,888 - Pf1N1B4_3584 0.14 +0.1 3,994,896 + Pf1N1B4_3584 0.14 +0.4 3,994,897 - -0.5 3,994,899 + Pf1N1B4_3584 0.14 -1.0 3,994,918 + Pf1N1B4_3584 0.15 -0.3 3,994,965 + +0.1 3,994,966 - Pf1N1B4_3584 0.17 -0.0 3,994,977 + Pf1N1B4_3584 0.17 -1.6 3,994,977 + Pf1N1B4_3584 0.17 +0.5 3,994,978 - Pf1N1B4_3584 0.17 -0.2 3,994,978 - Pf1N1B4_3584 0.17 +0.6 3,994,978 - -0.7 3,994,978 - Pf1N1B4_3584 0.17 -0.7 3,994,992 + Pf1N1B4_3584 0.17 +2.9 3,994,993 - Pf1N1B4_3584 0.18 +0.3 3,995,028 + Pf1N1B4_3584 0.19 +0.1 3,995,082 + Pf1N1B4_3584 0.21 -1.3 3,995,083 - Pf1N1B4_3584 0.21 -1.7 3,995,083 - Pf1N1B4_3584 0.21 -0.9 3,995,083 - Pf1N1B4_3584 0.21 -0.7 3,995,085 + Pf1N1B4_3584 0.21 -0.4 3,995,085 + Pf1N1B4_3584 0.21 -0.2 3,995,085 + Pf1N1B4_3584 0.21 +0.3 3,995,086 - Pf1N1B4_3584 0.21 -0.0 3,995,086 - Pf1N1B4_3584 0.21 +1.1 3,995,086 - Pf1N1B4_3584 0.21 +2.1 3,995,127 + Pf1N1B4_3584 0.22 +1.3 3,995,127 + Pf1N1B4_3584 0.22 -0.1 3,995,127 + Pf1N1B4_3584 0.22 +0.1 3,995,127 + Pf1N1B4_3584 0.22 -0.1 3,995,128 - Pf1N1B4_3584 0.22 -0.7 3,995,427 + Pf1N1B4_3584 0.32 +0.1 3,995,427 + Pf1N1B4_3584 0.32 -0.1 3,995,427 + Pf1N1B4_3584 0.32 -1.8 3,995,427 + Pf1N1B4_3584 0.32 -0.0 3,995,427 + Pf1N1B4_3584 0.32 -1.7 3,995,427 + Pf1N1B4_3584 0.32 +0.3 3,995,428 - Pf1N1B4_3584 0.32 -0.0 3,995,428 - -0.3 3,995,428 - Pf1N1B4_3584 0.32 -1.2 3,995,428 - Pf1N1B4_3584 0.32 -0.1 3,995,428 - Pf1N1B4_3584 0.32 -1.3 3,995,445 + Pf1N1B4_3584 0.33 -0.2 3,995,555 + Pf1N1B4_3584 0.37 -1.3 3,995,555 + Pf1N1B4_3584 0.37 +0.7 3,995,555 + +0.9 3,995,556 - Pf1N1B4_3584 0.37 -0.5 3,995,556 - Pf1N1B4_3584 0.37 -1.5 3,995,556 - Pf1N1B4_3584 0.37 -0.7 3,995,556 - Pf1N1B4_3584 0.37 +0.4 3,995,556 - Pf1N1B4_3584 0.37 -1.7 3,995,556 - Pf1N1B4_3584 0.37 -0.6 3,995,572 - Pf1N1B4_3584 0.37 -1.5 3,996,054 + Pf1N1B4_3584 0.54 +0.5 3,996,054 + +0.1 3,996,054 + +0.5 3,996,054 + Pf1N1B4_3584 0.54 -0.1 3,996,843 + Pf1N1B4_3584 0.80 -0.7 3,996,844 - Pf1N1B4_3584 0.81 +0.9 3,997,051 - Pf1N1B4_3584 0.88 +0.7 3,997,170 + +0.0 3,997,332 + +1.0 3,997,367 + +0.7 3,997,414 - -1.0 3,997,431 - +1.3 3,997,431 - +1.9 3,997,575 + -0.7 3,997,615 - Pf1N1B4_3585 0.17 -0.7 3,997,719 + Pf1N1B4_3585 0.27 -1.0 3,997,719 + Pf1N1B4_3585 0.27 -0.9 3,997,720 - Pf1N1B4_3585 0.27 +0.0 3,998,274 + Pf1N1B4_3585 0.83 -0.7 3,998,303 + Pf1N1B4_3585 0.86 +0.4 3,998,303 + Pf1N1B4_3585 0.86 -0.3
Or see this region's nucleotide sequence