Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3600

Experiment: Time16-FRS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3599 and Pf1N1B4_3600 are separated by 110 nucleotidesPf1N1B4_3600 and Pf1N1B4_3601 overlap by 4 nucleotidesPf1N1B4_3601 and Pf1N1B4_3602 overlap by 37 nucleotides Pf1N1B4_3599 - Flagellar regulatory protein FleQ, at 4,009,732 to 4,011,207 _3599 Pf1N1B4_3600 - Flagellar sensor histidine kinase FleS, at 4,011,318 to 4,012,541 _3600 Pf1N1B4_3601 - Flagellar regulatory protein FleQ, at 4,012,538 to 4,013,380 _3601 Pf1N1B4_3602 - Flagellar regulatory protein FleQ, at 4,013,344 to 4,013,931 _3602 Position (kb) 4011 4012 4013Strain fitness (log2 ratio) -2 -1 0 1 2at 4010.346 kb on + strand, within Pf1N1B4_3599at 4010.451 kb on + strand, within Pf1N1B4_3599at 4010.641 kb on + strand, within Pf1N1B4_3599at 4010.641 kb on + strand, within Pf1N1B4_3599at 4010.642 kb on - strand, within Pf1N1B4_3599at 4011.163 kb on + strandat 4011.164 kb on - strandat 4011.232 kb on + strandat 4011.233 kb on - strandat 4011.234 kb on + strandat 4011.234 kb on + strandat 4011.234 kb on + strandat 4011.264 kb on + strandat 4011.264 kb on + strandat 4011.264 kb on + strandat 4011.453 kb on + strand, within Pf1N1B4_3600at 4011.629 kb on + strand, within Pf1N1B4_3600at 4011.758 kb on - strand, within Pf1N1B4_3600at 4011.882 kb on - strand, within Pf1N1B4_3600at 4011.936 kb on + strand, within Pf1N1B4_3600at 4011.937 kb on - strand, within Pf1N1B4_3600at 4012.191 kb on - strand, within Pf1N1B4_3600at 4012.281 kb on + strand, within Pf1N1B4_3600at 4012.281 kb on + strand, within Pf1N1B4_3600at 4012.281 kb on + strand, within Pf1N1B4_3600at 4012.282 kb on - strand, within Pf1N1B4_3600at 4012.282 kb on - strandat 4012.282 kb on - strand, within Pf1N1B4_3600at 4012.346 kb on - strand, within Pf1N1B4_3600at 4012.380 kb on - strand, within Pf1N1B4_3600at 4012.564 kb on - strandat 4012.632 kb on - strand, within Pf1N1B4_3601at 4012.687 kb on + strand, within Pf1N1B4_3601at 4012.687 kb on + strand, within Pf1N1B4_3601at 4012.776 kb on + strand, within Pf1N1B4_3601at 4012.777 kb on - strand, within Pf1N1B4_3601at 4012.777 kb on - strand, within Pf1N1B4_3601at 4012.779 kb on + strand, within Pf1N1B4_3601at 4012.845 kb on - strand, within Pf1N1B4_3601at 4013.055 kb on - strand, within Pf1N1B4_3601at 4013.055 kb on - strand, within Pf1N1B4_3601at 4013.092 kb on + strand, within Pf1N1B4_3601at 4013.407 kb on + strand, within Pf1N1B4_3602at 4013.408 kb on - strand, within Pf1N1B4_3602at 4013.408 kb on - strand, within Pf1N1B4_3602at 4013.408 kb on - strand, within Pf1N1B4_3602

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Per-strain Table

Position Strand Gene LocusTag Fraction Time16-FRS
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4,010,346 + Pf1N1B4_3599 0.42 -0.9
4,010,451 + Pf1N1B4_3599 0.49 +0.5
4,010,641 + Pf1N1B4_3599 0.62 -0.1
4,010,641 + Pf1N1B4_3599 0.62 +0.5
4,010,642 - Pf1N1B4_3599 0.62 -1.1
4,011,163 + -1.5
4,011,164 - +1.3
4,011,232 + -0.3
4,011,233 - +0.3
4,011,234 + -0.0
4,011,234 + +0.3
4,011,234 + -2.0
4,011,264 + +0.0
4,011,264 + -0.6
4,011,264 + -0.5
4,011,453 + Pf1N1B4_3600 0.11 -0.4
4,011,629 + Pf1N1B4_3600 0.25 +0.9
4,011,758 - Pf1N1B4_3600 0.36 -0.7
4,011,882 - Pf1N1B4_3600 0.46 -0.2
4,011,936 + Pf1N1B4_3600 0.50 +0.4
4,011,937 - Pf1N1B4_3600 0.51 +0.6
4,012,191 - Pf1N1B4_3600 0.71 -0.5
4,012,281 + Pf1N1B4_3600 0.79 -0.4
4,012,281 + Pf1N1B4_3600 0.79 -0.2
4,012,281 + Pf1N1B4_3600 0.79 +0.4
4,012,282 - Pf1N1B4_3600 0.79 +1.1
4,012,282 - -1.0
4,012,282 - Pf1N1B4_3600 0.79 -0.0
4,012,346 - Pf1N1B4_3600 0.84 +1.2
4,012,380 - Pf1N1B4_3600 0.87 +2.3
4,012,564 - -0.1
4,012,632 - Pf1N1B4_3601 0.11 -0.8
4,012,687 + Pf1N1B4_3601 0.18 -0.8
4,012,687 + Pf1N1B4_3601 0.18 +0.5
4,012,776 + Pf1N1B4_3601 0.28 -0.4
4,012,777 - Pf1N1B4_3601 0.28 -0.4
4,012,777 - Pf1N1B4_3601 0.28 -1.0
4,012,779 + Pf1N1B4_3601 0.29 -0.3
4,012,845 - Pf1N1B4_3601 0.36 -0.7
4,013,055 - Pf1N1B4_3601 0.61 +0.9
4,013,055 - Pf1N1B4_3601 0.61 -1.3
4,013,092 + Pf1N1B4_3601 0.66 -0.6
4,013,407 + Pf1N1B4_3602 0.11 -1.1
4,013,408 - Pf1N1B4_3602 0.11 +0.7
4,013,408 - Pf1N1B4_3602 0.11 -0.3
4,013,408 - Pf1N1B4_3602 0.11 +0.8

Or see this region's nucleotide sequence