Experiment: Time16-FRS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3158 and Pf1N1B4_3159 are separated by 18 nucleotides Pf1N1B4_3159 and Pf1N1B4_3160 are separated by 104 nucleotides Pf1N1B4_3160 and Pf1N1B4_3161 are separated by 92 nucleotides Pf1N1B4_3161 and Pf1N1B4_3162 overlap by 39 nucleotides
Pf1N1B4_3158 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 3,536,103 to 3,536,987
_3158
Pf1N1B4_3159 - Histone acetyltransferase HPA2 and related acetyltransferases, at 3,537,006 to 3,537,479
_3159
Pf1N1B4_3160 - Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase, at 3,537,584 to 3,538,921
_3160
Pf1N1B4_3161 - hypothetical protein, at 3,539,014 to 3,539,250
_3161
Pf1N1B4_3162 - Kup system potassium uptake protein, at 3,539,212 to 3,541,113
_3162
Position (kb)
3537
3538
3539 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3536.847 kb on + strand, within Pf1N1B4_3158 at 3536.847 kb on + strand, within Pf1N1B4_3158 at 3536.847 kb on + strand at 3536.847 kb on + strand, within Pf1N1B4_3158 at 3536.848 kb on - strand, within Pf1N1B4_3158 at 3536.848 kb on - strand, within Pf1N1B4_3158 at 3536.929 kb on - strand at 3536.942 kb on + strand at 3536.943 kb on - strand at 3536.943 kb on - strand at 3536.943 kb on - strand at 3536.943 kb on - strand at 3536.943 kb on - strand at 3536.964 kb on - strand at 3536.964 kb on - strand at 3537.040 kb on - strand at 3537.062 kb on - strand, within Pf1N1B4_3159 at 3537.121 kb on + strand, within Pf1N1B4_3159 at 3537.121 kb on + strand, within Pf1N1B4_3159 at 3537.121 kb on + strand, within Pf1N1B4_3159 at 3537.121 kb on + strand, within Pf1N1B4_3159 at 3537.122 kb on - strand, within Pf1N1B4_3159 at 3537.122 kb on - strand, within Pf1N1B4_3159 at 3537.122 kb on - strand, within Pf1N1B4_3159 at 3537.122 kb on - strand, within Pf1N1B4_3159 at 3537.309 kb on - strand, within Pf1N1B4_3159 at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.370 kb on + strand at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.371 kb on - strand at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.436 kb on + strand at 3537.437 kb on - strand at 3537.526 kb on - strand at 3537.531 kb on + strand at 3537.531 kb on + strand at 3537.531 kb on + strand at 3537.532 kb on - strand at 3537.532 kb on - strand at 3537.572 kb on + strand at 3537.585 kb on + strand at 3537.586 kb on - strand at 3537.586 kb on - strand at 3537.586 kb on - strand at 3537.610 kb on - strand at 3537.961 kb on - strand, within Pf1N1B4_3160 at 3537.961 kb on - strand, within Pf1N1B4_3160 at 3538.138 kb on - strand, within Pf1N1B4_3160 at 3538.204 kb on - strand, within Pf1N1B4_3160 at 3538.204 kb on - strand at 3538.204 kb on - strand, within Pf1N1B4_3160 at 3538.318 kb on - strand, within Pf1N1B4_3160 at 3538.486 kb on - strand, within Pf1N1B4_3160 at 3538.510 kb on + strand, within Pf1N1B4_3160 at 3538.511 kb on - strand, within Pf1N1B4_3160 at 3538.511 kb on - strand, within Pf1N1B4_3160 at 3538.599 kb on + strand, within Pf1N1B4_3160 at 3538.637 kb on + strand, within Pf1N1B4_3160 at 3538.637 kb on + strand, within Pf1N1B4_3160 at 3538.638 kb on - strand, within Pf1N1B4_3160 at 3538.638 kb on - strand, within Pf1N1B4_3160 at 3538.638 kb on - strand at 3538.638 kb on - strand, within Pf1N1B4_3160 at 3538.792 kb on - strand at 3538.810 kb on - strand at 3538.981 kb on + strand at 3538.982 kb on - strand at 3538.982 kb on - strand at 3539.045 kb on - strand, within Pf1N1B4_3161 at 3539.045 kb on - strand at 3539.045 kb on - strand, within Pf1N1B4_3161 at 3539.056 kb on + strand, within Pf1N1B4_3161 at 3539.056 kb on + strand, within Pf1N1B4_3161 at 3539.057 kb on - strand, within Pf1N1B4_3161 at 3539.096 kb on - strand, within Pf1N1B4_3161 at 3539.106 kb on + strand, within Pf1N1B4_3161 at 3539.107 kb on - strand, within Pf1N1B4_3161 at 3539.116 kb on - strand, within Pf1N1B4_3161 at 3539.137 kb on - strand at 3539.137 kb on - strand, within Pf1N1B4_3161 at 3539.165 kb on + strand, within Pf1N1B4_3161 at 3539.166 kb on - strand, within Pf1N1B4_3161 at 3539.166 kb on - strand, within Pf1N1B4_3161 at 3539.175 kb on + strand, within Pf1N1B4_3161 at 3539.175 kb on + strand, within Pf1N1B4_3161 at 3539.175 kb on + strand at 3539.175 kb on + strand, within Pf1N1B4_3161 at 3539.175 kb on + strand, within Pf1N1B4_3161 at 3539.176 kb on - strand, within Pf1N1B4_3161 at 3539.176 kb on - strand, within Pf1N1B4_3161 at 3539.176 kb on - strand, within Pf1N1B4_3161 at 3539.176 kb on - strand, within Pf1N1B4_3161 at 3539.176 kb on - strand, within Pf1N1B4_3161 at 3539.176 kb on - strand, within Pf1N1B4_3161 at 3539.177 kb on + strand, within Pf1N1B4_3161 at 3539.177 kb on + strand, within Pf1N1B4_3161 at 3539.177 kb on + strand, within Pf1N1B4_3161 at 3539.178 kb on - strand, within Pf1N1B4_3161 at 3539.178 kb on - strand, within Pf1N1B4_3161 at 3539.178 kb on - strand, within Pf1N1B4_3161 at 3539.178 kb on - strand, within Pf1N1B4_3161 at 3539.178 kb on - strand, within Pf1N1B4_3161 at 3539.178 kb on - strand at 3539.178 kb on - strand, within Pf1N1B4_3161 at 3539.178 kb on - strand, within Pf1N1B4_3161 at 3539.178 kb on - strand, within Pf1N1B4_3161 at 3539.200 kb on - strand, within Pf1N1B4_3161 at 3539.226 kb on + strand at 3539.226 kb on + strand at 3539.226 kb on + strand at 3539.226 kb on + strand at 3539.227 kb on - strand at 3539.227 kb on - strand at 3539.227 kb on - strand at 3539.326 kb on + strand at 3539.326 kb on + strand at 3539.326 kb on + strand at 3539.326 kb on + strand at 3539.326 kb on + strand at 3539.327 kb on - strand at 3539.327 kb on - strand at 3539.327 kb on - strand at 3539.357 kb on - strand at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.440 kb on + strand, within Pf1N1B4_3162 at 3539.441 kb on - strand, within Pf1N1B4_3162 at 3539.441 kb on - strand, within Pf1N1B4_3162 at 3539.441 kb on - strand, within Pf1N1B4_3162 at 3539.441 kb on - strand, within Pf1N1B4_3162 at 3539.441 kb on - strand, within Pf1N1B4_3162 at 3539.441 kb on - strand, within Pf1N1B4_3162 at 3539.541 kb on + strand, within Pf1N1B4_3162 at 3539.541 kb on + strand, within Pf1N1B4_3162 at 3539.584 kb on + strand, within Pf1N1B4_3162 at 3539.585 kb on - strand at 3539.800 kb on + strand, within Pf1N1B4_3162 at 3539.801 kb on - strand, within Pf1N1B4_3162 at 3539.801 kb on - strand, within Pf1N1B4_3162
Per-strain Table
Position Strand Gene LocusTag Fraction Time16-FRS remove 3,536,847 + Pf1N1B4_3158 0.84 +0.6 3,536,847 + Pf1N1B4_3158 0.84 -0.1 3,536,847 + +2.6 3,536,847 + Pf1N1B4_3158 0.84 -0.1 3,536,848 - Pf1N1B4_3158 0.84 -0.0 3,536,848 - Pf1N1B4_3158 0.84 -0.4 3,536,929 - +0.7 3,536,942 + +0.5 3,536,943 - -1.0 3,536,943 - -0.2 3,536,943 - -1.0 3,536,943 - +0.2 3,536,943 - -0.0 3,536,964 - +0.5 3,536,964 - -0.0 3,537,040 - +0.1 3,537,062 - Pf1N1B4_3159 0.12 +0.2 3,537,121 + Pf1N1B4_3159 0.24 -1.9 3,537,121 + Pf1N1B4_3159 0.24 -0.8 3,537,121 + Pf1N1B4_3159 0.24 +1.1 3,537,121 + Pf1N1B4_3159 0.24 +0.6 3,537,122 - Pf1N1B4_3159 0.24 -0.4 3,537,122 - Pf1N1B4_3159 0.24 -1.4 3,537,122 - Pf1N1B4_3159 0.24 +0.4 3,537,122 - Pf1N1B4_3159 0.24 +0.7 3,537,309 - Pf1N1B4_3159 0.64 -0.2 3,537,370 + Pf1N1B4_3159 0.77 -1.2 3,537,370 + Pf1N1B4_3159 0.77 -0.6 3,537,370 + Pf1N1B4_3159 0.77 -0.5 3,537,370 + +0.2 3,537,370 + Pf1N1B4_3159 0.77 -0.5 3,537,370 + Pf1N1B4_3159 0.77 +0.7 3,537,371 - +1.0 3,537,371 - Pf1N1B4_3159 0.77 -2.4 3,537,371 - Pf1N1B4_3159 0.77 -0.2 3,537,371 - Pf1N1B4_3159 0.77 -0.6 3,537,371 - Pf1N1B4_3159 0.77 +0.7 3,537,371 - Pf1N1B4_3159 0.77 +1.6 3,537,371 - Pf1N1B4_3159 0.77 -1.8 3,537,371 - Pf1N1B4_3159 0.77 +0.6 3,537,371 - Pf1N1B4_3159 0.77 +0.7 3,537,371 - Pf1N1B4_3159 0.77 +0.9 3,537,436 + -0.4 3,537,437 - -0.1 3,537,526 - +0.3 3,537,531 + +2.3 3,537,531 + -1.1 3,537,531 + +0.7 3,537,532 - -0.0 3,537,532 - -0.2 3,537,572 + -1.3 3,537,585 + -0.0 3,537,586 - +0.6 3,537,586 - +0.6 3,537,586 - -1.2 3,537,610 - -0.6 3,537,961 - Pf1N1B4_3160 0.28 -0.1 3,537,961 - Pf1N1B4_3160 0.28 -1.8 3,538,138 - Pf1N1B4_3160 0.41 -1.1 3,538,204 - Pf1N1B4_3160 0.46 +1.0 3,538,204 - -0.0 3,538,204 - Pf1N1B4_3160 0.46 -2.0 3,538,318 - Pf1N1B4_3160 0.55 -1.7 3,538,486 - Pf1N1B4_3160 0.67 -1.2 3,538,510 + Pf1N1B4_3160 0.69 -0.0 3,538,511 - Pf1N1B4_3160 0.69 -0.0 3,538,511 - Pf1N1B4_3160 0.69 -0.0 3,538,599 + Pf1N1B4_3160 0.76 -0.3 3,538,637 + Pf1N1B4_3160 0.79 -1.0 3,538,637 + Pf1N1B4_3160 0.79 -0.7 3,538,638 - Pf1N1B4_3160 0.79 -0.6 3,538,638 - Pf1N1B4_3160 0.79 -0.6 3,538,638 - +1.6 3,538,638 - Pf1N1B4_3160 0.79 -0.3 3,538,792 - -0.4 3,538,810 - -0.2 3,538,981 + -0.9 3,538,982 - -0.4 3,538,982 - +0.3 3,539,045 - Pf1N1B4_3161 0.13 -0.2 3,539,045 - -0.3 3,539,045 - Pf1N1B4_3161 0.13 +0.3 3,539,056 + Pf1N1B4_3161 0.18 +0.9 3,539,056 + Pf1N1B4_3161 0.18 +0.8 3,539,057 - Pf1N1B4_3161 0.18 -0.1 3,539,096 - Pf1N1B4_3161 0.35 -1.9 3,539,106 + Pf1N1B4_3161 0.39 +0.2 3,539,107 - Pf1N1B4_3161 0.39 +0.7 3,539,116 - Pf1N1B4_3161 0.43 +0.2 3,539,137 - +0.6 3,539,137 - Pf1N1B4_3161 0.52 -0.3 3,539,165 + Pf1N1B4_3161 0.64 +0.5 3,539,166 - Pf1N1B4_3161 0.64 +1.6 3,539,166 - Pf1N1B4_3161 0.64 +0.2 3,539,175 + Pf1N1B4_3161 0.68 -0.9 3,539,175 + Pf1N1B4_3161 0.68 -0.8 3,539,175 + -1.0 3,539,175 + Pf1N1B4_3161 0.68 -0.6 3,539,175 + Pf1N1B4_3161 0.68 -0.2 3,539,176 - Pf1N1B4_3161 0.68 +1.3 3,539,176 - Pf1N1B4_3161 0.68 +0.2 3,539,176 - Pf1N1B4_3161 0.68 +1.0 3,539,176 - Pf1N1B4_3161 0.68 -0.2 3,539,176 - Pf1N1B4_3161 0.68 +0.7 3,539,176 - Pf1N1B4_3161 0.68 -1.0 3,539,177 + Pf1N1B4_3161 0.69 -1.2 3,539,177 + Pf1N1B4_3161 0.69 -0.3 3,539,177 + Pf1N1B4_3161 0.69 -0.1 3,539,178 - Pf1N1B4_3161 0.69 -0.3 3,539,178 - Pf1N1B4_3161 0.69 -0.2 3,539,178 - Pf1N1B4_3161 0.69 +1.6 3,539,178 - Pf1N1B4_3161 0.69 +0.3 3,539,178 - Pf1N1B4_3161 0.69 -2.0 3,539,178 - +2.6 3,539,178 - Pf1N1B4_3161 0.69 -0.2 3,539,178 - Pf1N1B4_3161 0.69 -0.3 3,539,178 - Pf1N1B4_3161 0.69 +0.7 3,539,200 - Pf1N1B4_3161 0.78 +0.8 3,539,226 + -0.4 3,539,226 + +0.6 3,539,226 + +0.1 3,539,226 + -1.0 3,539,227 - +0.2 3,539,227 - -0.1 3,539,227 - -0.8 3,539,326 + -0.9 3,539,326 + -0.2 3,539,326 + -0.8 3,539,326 + +0.9 3,539,326 + -1.4 3,539,327 - +0.8 3,539,327 - -1.9 3,539,327 - +1.2 3,539,357 - +0.3 3,539,440 + Pf1N1B4_3162 0.12 -0.2 3,539,440 + Pf1N1B4_3162 0.12 +0.2 3,539,440 + Pf1N1B4_3162 0.12 -1.2 3,539,440 + Pf1N1B4_3162 0.12 -2.0 3,539,440 + Pf1N1B4_3162 0.12 -0.3 3,539,440 + Pf1N1B4_3162 0.12 -0.2 3,539,440 + Pf1N1B4_3162 0.12 -2.3 3,539,440 + Pf1N1B4_3162 0.12 -2.2 3,539,440 + Pf1N1B4_3162 0.12 +0.5 3,539,440 + Pf1N1B4_3162 0.12 -0.4 3,539,441 - Pf1N1B4_3162 0.12 +1.4 3,539,441 - Pf1N1B4_3162 0.12 -0.0 3,539,441 - Pf1N1B4_3162 0.12 -0.3 3,539,441 - Pf1N1B4_3162 0.12 +0.2 3,539,441 - Pf1N1B4_3162 0.12 -1.2 3,539,441 - Pf1N1B4_3162 0.12 +0.6 3,539,541 + Pf1N1B4_3162 0.17 +0.8 3,539,541 + Pf1N1B4_3162 0.17 -0.5 3,539,584 + Pf1N1B4_3162 0.20 +0.6 3,539,585 - -0.0 3,539,800 + Pf1N1B4_3162 0.31 -0.0 3,539,801 - Pf1N1B4_3162 0.31 +0.7 3,539,801 - Pf1N1B4_3162 0.31 -0.4
Or see this region's nucleotide sequence