Experiment: Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03540 and H281DRAFT_03541 overlap by 4 nucleotides H281DRAFT_03541 and H281DRAFT_03542 are separated by 52 nucleotides
H281DRAFT_03540: H281DRAFT_03540 - succinate semialdehyde dehydrogenase (EC 1.2.1.16), at 40,894 to 42,333
_03540
H281DRAFT_03541: H281DRAFT_03541 - Predicted oxidoreductase, at 42,330 to 43,367
_03541
H281DRAFT_03542: H281DRAFT_03542 - 5,6,7,8-tetrahydromethanopterin hydro-lyase, at 43,420 to 43,944
_03542
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1 at 41.447 kb on + strand, within H281DRAFT_03540 at 41.447 kb on + strand, within H281DRAFT_03540 at 41.677 kb on + strand, within H281DRAFT_03540 at 41.677 kb on + strand at 41.677 kb on + strand, within H281DRAFT_03540 at 41.678 kb on - strand, within H281DRAFT_03540 at 41.678 kb on - strand, within H281DRAFT_03540 at 41.972 kb on + strand, within H281DRAFT_03540 at 41.973 kb on - strand, within H281DRAFT_03540 at 42.223 kb on - strand at 42.287 kb on + strand at 42.287 kb on + strand at 42.287 kb on + strand at 42.305 kb on + strand at 42.305 kb on + strand at 42.410 kb on - strand at 42.436 kb on + strand, within H281DRAFT_03541 at 42.437 kb on - strand, within H281DRAFT_03541 at 42.492 kb on + strand, within H281DRAFT_03541 at 42.711 kb on + strand, within H281DRAFT_03541 at 42.889 kb on + strand, within H281DRAFT_03541 at 42.889 kb on + strand, within H281DRAFT_03541 at 42.889 kb on + strand, within H281DRAFT_03541 at 42.910 kb on + strand, within H281DRAFT_03541 at 42.910 kb on + strand, within H281DRAFT_03541 at 42.911 kb on - strand, within H281DRAFT_03541 at 42.997 kb on + strand, within H281DRAFT_03541 at 42.997 kb on + strand, within H281DRAFT_03541 at 42.998 kb on - strand, within H281DRAFT_03541 at 43.009 kb on + strand, within H281DRAFT_03541 at 43.193 kb on - strand, within H281DRAFT_03541 at 43.360 kb on + strand at 43.360 kb on + strand at 43.361 kb on - strand at 43.440 kb on - strand at 43.440 kb on - strand at 43.440 kb on - strand at 43.440 kb on - strand at 43.872 kb on + strand, within H281DRAFT_03542 at 43.873 kb on - strand, within H281DRAFT_03542 at 43.873 kb on - strand, within H281DRAFT_03542 at 44.003 kb on + strand at 44.004 kb on - strand at 44.004 kb on - strand at 44.004 kb on - strand at 44.004 kb on - strand at 44.004 kb on - strand at 44.026 kb on + strand at 44.027 kb on - strand at 44.104 kb on + strand at 44.105 kb on - strand at 44.105 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days remove 41,447 + H281DRAFT_03540 0.38 -0.1 41,447 + H281DRAFT_03540 0.38 +0.1 41,677 + H281DRAFT_03540 0.54 -0.9 41,677 + -1.6 41,677 + H281DRAFT_03540 0.54 +0.2 41,678 - H281DRAFT_03540 0.54 +0.8 41,678 - H281DRAFT_03540 0.54 +0.1 41,972 + H281DRAFT_03540 0.75 +0.2 41,973 - H281DRAFT_03540 0.75 -1.1 42,223 - +0.4 42,287 + +0.0 42,287 + +0.5 42,287 + -0.4 42,305 + +0.4 42,305 + -0.6 42,410 - -0.2 42,436 + H281DRAFT_03541 0.10 -0.2 42,437 - H281DRAFT_03541 0.10 +0.7 42,492 + H281DRAFT_03541 0.16 +0.2 42,711 + H281DRAFT_03541 0.37 -0.5 42,889 + H281DRAFT_03541 0.54 +0.0 42,889 + H281DRAFT_03541 0.54 -0.5 42,889 + H281DRAFT_03541 0.54 +0.5 42,910 + H281DRAFT_03541 0.56 -0.5 42,910 + H281DRAFT_03541 0.56 +0.4 42,911 - H281DRAFT_03541 0.56 -0.2 42,997 + H281DRAFT_03541 0.64 -0.1 42,997 + H281DRAFT_03541 0.64 -0.2 42,998 - H281DRAFT_03541 0.64 -0.1 43,009 + H281DRAFT_03541 0.65 +1.1 43,193 - H281DRAFT_03541 0.83 -0.0 43,360 + -0.3 43,360 + -0.1 43,361 - +0.1 43,440 - -0.1 43,440 - -0.0 43,440 - -0.4 43,440 - -0.8 43,872 + H281DRAFT_03542 0.86 +0.3 43,873 - H281DRAFT_03542 0.86 +0.3 43,873 - H281DRAFT_03542 0.86 +0.4 44,003 + -0.3 44,004 - +0.3 44,004 - -0.4 44,004 - +0.0 44,004 - +0.1 44,004 - +0.3 44,026 + -2.3 44,027 - +0.5 44,104 + -0.5 44,105 - +0.1 44,105 - -0.4
Or see this region's nucleotide sequence