Experiment: Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04549 and H281DRAFT_04550 overlap by 4 nucleotides H281DRAFT_04550 and H281DRAFT_04551 are separated by 355 nucleotides H281DRAFT_04551 and H281DRAFT_04552 are separated by 111 nucleotides H281DRAFT_04552 and H281DRAFT_04553 are separated by 112 nucleotides
H281DRAFT_04549: H281DRAFT_04549 - Protein of unknown function (DUF3619), at 281,135 to 281,563
_04549
H281DRAFT_04550: H281DRAFT_04550 - RNA polymerase, sigma-24 subunit, RpoE, at 281,560 to 282,123
_04550
H281DRAFT_04551: H281DRAFT_04551 - acetolactate synthase, large subunit, at 282,479 to 284,242
_04551
H281DRAFT_04552: H281DRAFT_04552 - acetolactate synthase, small subunit, at 284,354 to 284,845
_04552
H281DRAFT_04553: H281DRAFT_04553 - ketol-acid reductoisomerase, at 284,958 to 285,974
_04553
Position (kb) 282
283
284
285 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 281.563 kb on + strand at 281.564 kb on - strand at 281.679 kb on + strand, within H281DRAFT_04550 at 281.680 kb on - strand, within H281DRAFT_04550 at 281.680 kb on - strand, within H281DRAFT_04550 at 281.680 kb on - strand, within H281DRAFT_04550 at 281.725 kb on + strand, within H281DRAFT_04550 at 281.889 kb on + strand, within H281DRAFT_04550 at 281.889 kb on + strand, within H281DRAFT_04550 at 281.889 kb on + strand, within H281DRAFT_04550 at 281.889 kb on + strand, within H281DRAFT_04550 at 281.889 kb on + strand, within H281DRAFT_04550 at 281.890 kb on - strand, within H281DRAFT_04550 at 281.890 kb on - strand, within H281DRAFT_04550 at 281.890 kb on - strand, within H281DRAFT_04550 at 281.890 kb on - strand, within H281DRAFT_04550 at 281.890 kb on - strand, within H281DRAFT_04550 at 281.940 kb on + strand, within H281DRAFT_04550 at 282.157 kb on - strand at 282.346 kb on + strand at 282.346 kb on + strand at 282.346 kb on + strand at 284.238 kb on + strand at 284.238 kb on + strand at 284.323 kb on + strand at 284.324 kb on - strand at 284.365 kb on + strand at 284.711 kb on + strand, within H281DRAFT_04552 at 284.711 kb on + strand, within H281DRAFT_04552 at 284.711 kb on + strand, within H281DRAFT_04552 at 284.711 kb on + strand, within H281DRAFT_04552 at 284.914 kb on - strand at 285.223 kb on - strand, within H281DRAFT_04553
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days remove 281,563 + +1.2 281,564 - +0.1 281,679 + H281DRAFT_04550 0.21 -2.2 281,680 - H281DRAFT_04550 0.21 -1.4 281,680 - H281DRAFT_04550 0.21 -0.8 281,680 - H281DRAFT_04550 0.21 -0.7 281,725 + H281DRAFT_04550 0.29 -1.7 281,889 + H281DRAFT_04550 0.58 -0.7 281,889 + H281DRAFT_04550 0.58 -1.5 281,889 + H281DRAFT_04550 0.58 +1.4 281,889 + H281DRAFT_04550 0.58 -2.3 281,889 + H281DRAFT_04550 0.58 -1.4 281,890 - H281DRAFT_04550 0.59 -1.5 281,890 - H281DRAFT_04550 0.59 -1.2 281,890 - H281DRAFT_04550 0.59 -0.4 281,890 - H281DRAFT_04550 0.59 -1.1 281,890 - H281DRAFT_04550 0.59 -2.4 281,940 + H281DRAFT_04550 0.67 -0.9 282,157 - +0.6 282,346 + +0.1 282,346 + -0.2 282,346 + -0.1 284,238 + +1.2 284,238 + -0.3 284,323 + +1.1 284,324 - -0.1 284,365 + -0.3 284,711 + H281DRAFT_04552 0.73 -0.3 284,711 + H281DRAFT_04552 0.73 -1.0 284,711 + H281DRAFT_04552 0.73 -0.4 284,711 + H281DRAFT_04552 0.73 -2.1 284,914 - -4.3 285,223 - H281DRAFT_04553 0.26 -3.2
Or see this region's nucleotide sequence