Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02101 and H281DRAFT_02102 are separated by 61 nucleotides H281DRAFT_02102 and H281DRAFT_02103 are separated by 4 nucleotides H281DRAFT_02103 and H281DRAFT_02104 overlap by 4 nucleotides H281DRAFT_02104 and H281DRAFT_02105 overlap by 4 nucleotides
H281DRAFT_02101: H281DRAFT_02101 - hydroxymethylglutaryl-CoA lyase, at 227,971 to 228,897
_02101
H281DRAFT_02102: H281DRAFT_02102 - Cys-tRNA(Pro) deacylase, prolyl-tRNA editing enzyme YbaK/EbsC, at 228,959 to 229,480
_02102
H281DRAFT_02103: H281DRAFT_02103 - hypothetical protein, at 229,485 to 229,712
_02103
H281DRAFT_02104: H281DRAFT_02104 - Acyl dehydratase, at 229,709 to 230,194
_02104
H281DRAFT_02105: H281DRAFT_02105 - Glyoxylase, beta-lactamase superfamily II, at 230,191 to 231,129
_02105
Position (kb)
229
230
231 Strain fitness (log2 ratio)
-1
0
1
2 at 228.739 kb on + strand, within H281DRAFT_02101 at 228.739 kb on + strand, within H281DRAFT_02101 at 228.901 kb on + strand at 228.902 kb on - strand at 228.942 kb on + strand at 229.225 kb on - strand, within H281DRAFT_02102 at 229.287 kb on - strand, within H281DRAFT_02102 at 229.287 kb on - strand, within H281DRAFT_02102 at 229.624 kb on - strand, within H281DRAFT_02103 at 229.838 kb on + strand, within H281DRAFT_02104 at 229.839 kb on - strand, within H281DRAFT_02104 at 229.961 kb on - strand, within H281DRAFT_02104 at 230.028 kb on - strand, within H281DRAFT_02104 at 230.028 kb on - strand, within H281DRAFT_02104 at 230.191 kb on - strand at 230.790 kb on + strand, within H281DRAFT_02105 at 231.127 kb on + strand at 231.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 228,739 + H281DRAFT_02101 0.83 +0.2 228,739 + H281DRAFT_02101 0.83 -0.2 228,901 + -0.1 228,902 - +0.5 228,942 + -0.3 229,225 - H281DRAFT_02102 0.51 +0.2 229,287 - H281DRAFT_02102 0.63 -0.2 229,287 - H281DRAFT_02102 0.63 -0.4 229,624 - H281DRAFT_02103 0.61 +0.3 229,838 + H281DRAFT_02104 0.27 +0.3 229,839 - H281DRAFT_02104 0.27 +1.9 229,961 - H281DRAFT_02104 0.52 +0.7 230,028 - H281DRAFT_02104 0.66 +0.2 230,028 - H281DRAFT_02104 0.66 +0.1 230,191 - +0.3 230,790 + H281DRAFT_02105 0.64 -0.1 231,127 + +1.5 231,135 + +0.0
Or see this region's nucleotide sequence