Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01199 and H281DRAFT_01200 overlap by 4 nucleotides H281DRAFT_01200 and H281DRAFT_01201 overlap by 4 nucleotides H281DRAFT_01201 and H281DRAFT_01202 are separated by 28 nucleotides
H281DRAFT_01199: H281DRAFT_01199 - Enoyl-CoA hydratase/carnithine racemase, at 36,599 to 37,759
_01199
H281DRAFT_01200: H281DRAFT_01200 - short chain enoyl-CoA hydratase (EC 4.2.1.17), at 37,756 to 38,592
_01200
H281DRAFT_01201: H281DRAFT_01201 - 3-hydroxyisobutyrate dehydrogenase, at 38,589 to 39,518
_01201
H281DRAFT_01202: H281DRAFT_01202 - methylmalonate-semialdehyde dehydrogenase [acylating], at 39,547 to 41,055
_01202
Position (kb)
37
38
39 Strain fitness (log2 ratio)
-1
0
1 at 36.842 kb on + strand, within H281DRAFT_01199 at 36.843 kb on - strand, within H281DRAFT_01199 at 36.865 kb on - strand, within H281DRAFT_01199 at 36.865 kb on - strand, within H281DRAFT_01199 at 36.865 kb on - strand, within H281DRAFT_01199 at 36.900 kb on + strand at 36.901 kb on - strand, within H281DRAFT_01199 at 37.224 kb on + strand, within H281DRAFT_01199 at 37.224 kb on + strand, within H281DRAFT_01199 at 38.447 kb on - strand, within H281DRAFT_01200 at 38.576 kb on + strand at 38.648 kb on - strand at 38.864 kb on + strand, within H281DRAFT_01201 at 38.867 kb on - strand, within H281DRAFT_01201 at 38.906 kb on + strand, within H281DRAFT_01201 at 38.907 kb on - strand, within H281DRAFT_01201 at 39.092 kb on - strand, within H281DRAFT_01201 at 39.436 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 36,842 + H281DRAFT_01199 0.21 +0.2 36,843 - H281DRAFT_01199 0.21 +0.2 36,865 - H281DRAFT_01199 0.23 +0.1 36,865 - H281DRAFT_01199 0.23 +0.2 36,865 - H281DRAFT_01199 0.23 +0.2 36,900 + -0.2 36,901 - H281DRAFT_01199 0.26 +0.1 37,224 + H281DRAFT_01199 0.54 +0.4 37,224 + H281DRAFT_01199 0.54 -0.2 38,447 - H281DRAFT_01200 0.83 -0.1 38,576 + -0.2 38,648 - +1.1 38,864 + H281DRAFT_01201 0.30 +1.6 38,867 - H281DRAFT_01201 0.30 -0.4 38,906 + H281DRAFT_01201 0.34 -0.5 38,907 - H281DRAFT_01201 0.34 +0.1 39,092 - H281DRAFT_01201 0.54 -0.3 39,436 - +0.5
Or see this region's nucleotide sequence