Experiment: McCas_H2_complete rubidium chloride 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06030 and MMP_RS06035 are separated by 118 nucleotides MMP_RS06035 and MMP_RS06040 are separated by 11 nucleotides MMP_RS06040 and MMP_RS06045 are separated by 29 nucleotides
MMP_RS06030: MMP_RS06030 - SufD family Fe-S cluster assembly protein, at 1,152,534 to 1,153,790
_RS06030
MMP_RS06035: MMP_RS06035 - glycosyltransferase family 2 protein, at 1,153,909 to 1,154,601
_RS06035
MMP_RS06040: MMP_RS06040 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,154,613 to 1,155,227
_RS06040
MMP_RS06045: MMP_RS06045 - DNA polymerase, at 1,155,257 to 1,155,808
_RS06045
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1153.048 kb on - strand at 1153.049 kb on + strand at 1153.062 kb on + strand at 1153.064 kb on - strand at 1153.143 kb on + strand at 1153.220 kb on - strand at 1153.221 kb on + strand at 1153.319 kb on + strand at 1153.454 kb on + strand at 1153.661 kb on - strand at 1153.688 kb on + strand at 1153.756 kb on - strand at 1153.785 kb on + strand at 1153.788 kb on - strand at 1153.788 kb on - strand at 1153.828 kb on - strand at 1153.843 kb on - strand at 1153.847 kb on - strand at 1153.848 kb on + strand at 1153.864 kb on - strand at 1153.864 kb on - strand at 1153.865 kb on + strand at 1153.869 kb on + strand at 1153.906 kb on + strand at 1153.935 kb on + strand at 1153.946 kb on + strand at 1154.016 kb on + strand, within MMP_RS06035 at 1154.016 kb on + strand, within MMP_RS06035 at 1154.070 kb on + strand, within MMP_RS06035 at 1154.089 kb on + strand, within MMP_RS06035 at 1154.091 kb on - strand, within MMP_RS06035 at 1154.144 kb on + strand, within MMP_RS06035 at 1154.157 kb on + strand, within MMP_RS06035 at 1154.222 kb on - strand, within MMP_RS06035 at 1154.257 kb on + strand, within MMP_RS06035 at 1154.287 kb on + strand, within MMP_RS06035 at 1154.335 kb on - strand, within MMP_RS06035 at 1154.340 kb on + strand, within MMP_RS06035 at 1154.433 kb on - strand, within MMP_RS06035 at 1154.437 kb on - strand, within MMP_RS06035 at 1154.444 kb on - strand, within MMP_RS06035 at 1154.445 kb on + strand, within MMP_RS06035 at 1154.445 kb on + strand, within MMP_RS06035 at 1154.515 kb on - strand, within MMP_RS06035 at 1154.516 kb on + strand, within MMP_RS06035 at 1154.516 kb on + strand, within MMP_RS06035 at 1154.518 kb on + strand, within MMP_RS06035 at 1154.608 kb on - strand at 1154.653 kb on - strand at 1154.663 kb on + strand at 1154.669 kb on - strand at 1154.838 kb on - strand at 1154.877 kb on + strand at 1155.033 kb on - strand at 1155.034 kb on + strand at 1155.077 kb on + strand at 1155.077 kb on + strand at 1155.086 kb on - strand at 1155.086 kb on - strand at 1155.197 kb on + strand at 1155.197 kb on + strand at 1155.209 kb on + strand at 1155.209 kb on + strand at 1155.209 kb on + strand at 1155.243 kb on + strand at 1155.249 kb on - strand at 1155.250 kb on + strand at 1155.250 kb on + strand at 1155.250 kb on + strand at 1155.253 kb on - strand at 1155.372 kb on + strand, within MMP_RS06045 at 1155.375 kb on + strand, within MMP_RS06045 at 1155.420 kb on + strand, within MMP_RS06045 at 1155.483 kb on + strand, within MMP_RS06045 at 1155.488 kb on + strand, within MMP_RS06045 at 1155.538 kb on - strand, within MMP_RS06045
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete rubidium chloride 125 mM remove 1,153,048 - +0.8 1,153,049 + -3.7 1,153,062 + -0.8 1,153,064 - +1.0 1,153,143 + -1.8 1,153,220 - +0.2 1,153,221 + -0.2 1,153,319 + +1.1 1,153,454 + -0.2 1,153,661 - -3.4 1,153,688 + -1.5 1,153,756 - +1.3 1,153,785 + +0.0 1,153,788 - +0.4 1,153,788 - -0.5 1,153,828 - +0.9 1,153,843 - -0.7 1,153,847 - -1.2 1,153,848 + +0.1 1,153,864 - -0.1 1,153,864 - -0.2 1,153,865 + +0.2 1,153,869 + +0.1 1,153,906 + -2.5 1,153,935 + -0.4 1,153,946 + -1.1 1,154,016 + MMP_RS06035 0.15 -1.1 1,154,016 + MMP_RS06035 0.15 -3.5 1,154,070 + MMP_RS06035 0.23 +0.4 1,154,089 + MMP_RS06035 0.26 -0.7 1,154,091 - MMP_RS06035 0.26 -0.2 1,154,144 + MMP_RS06035 0.34 -0.3 1,154,157 + MMP_RS06035 0.36 -0.5 1,154,222 - MMP_RS06035 0.45 -0.2 1,154,257 + MMP_RS06035 0.50 -0.7 1,154,287 + MMP_RS06035 0.55 +0.4 1,154,335 - MMP_RS06035 0.61 -1.5 1,154,340 + MMP_RS06035 0.62 +0.1 1,154,433 - MMP_RS06035 0.76 -0.2 1,154,437 - MMP_RS06035 0.76 -1.8 1,154,444 - MMP_RS06035 0.77 -0.1 1,154,445 + MMP_RS06035 0.77 -0.7 1,154,445 + MMP_RS06035 0.77 -0.4 1,154,515 - MMP_RS06035 0.87 -0.4 1,154,516 + MMP_RS06035 0.88 -0.9 1,154,516 + MMP_RS06035 0.88 -0.6 1,154,518 + MMP_RS06035 0.88 -0.3 1,154,608 - -1.4 1,154,653 - -1.8 1,154,663 + -4.3 1,154,669 - -1.5 1,154,838 - -1.6 1,154,877 + -1.4 1,155,033 - -2.4 1,155,034 + -0.6 1,155,077 + -4.3 1,155,077 + -0.3 1,155,086 - +0.5 1,155,086 - -3.9 1,155,197 + +0.8 1,155,197 + -0.3 1,155,209 + +0.2 1,155,209 + -0.1 1,155,209 + -1.6 1,155,243 + +0.7 1,155,249 - +0.4 1,155,250 + -0.6 1,155,250 + +0.7 1,155,250 + +1.7 1,155,253 - +0.1 1,155,372 + MMP_RS06045 0.21 +0.1 1,155,375 + MMP_RS06045 0.21 +0.2 1,155,420 + MMP_RS06045 0.30 +2.0 1,155,483 + MMP_RS06045 0.41 +0.7 1,155,488 + MMP_RS06045 0.42 +0.8 1,155,538 - MMP_RS06045 0.51 +0.3
Or see this region's nucleotide sequence