Experiment: McCas_H2_complete rubidium chloride 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03110 and MMP_RS03115 are separated by 36 nucleotides MMP_RS03115 and MMP_RS03120 are separated by 10 nucleotides MMP_RS03120 and MMP_RS03125 overlap by 4 nucleotides MMP_RS03125 and MMP_RS03130 are separated by 76 nucleotides MMP_RS03130 and MMP_RS03135 are separated by 18 nucleotides
MMP_RS03110: MMP_RS03110 - glycosyltransferase family 2 protein, at 587,336 to 588,031
_RS03110
MMP_RS03115: MMP_RS03115 - hypothetical protein, at 588,068 to 588,727
_RS03115
MMP_RS03120: MMP_RS03120 - hypothetical protein, at 588,738 to 588,920
_RS03120
MMP_RS03125: MMP_RS03125 - cell division ATPase MinD, at 588,917 to 589,702
_RS03125
MMP_RS03130: MMP_RS03130 - hypothetical protein, at 589,779 to 590,345
_RS03130
MMP_RS03135: MMP_RS03135 - O-phosphoseryl-tRNA(Sec) selenium transferase, at 590,364 to 591,674
_RS03135
Position (kb) 588
589
590 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 587.920 kb on - strand, within MMP_RS03110 at 587.939 kb on - strand, within MMP_RS03110 at 587.961 kb on + strand, within MMP_RS03110 at 588.047 kb on + strand at 588.065 kb on + strand at 588.101 kb on - strand at 588.101 kb on - strand at 588.101 kb on - strand at 588.101 kb on - strand at 588.102 kb on + strand at 588.102 kb on + strand at 588.104 kb on - strand at 588.106 kb on - strand at 588.107 kb on + strand at 588.110 kb on - strand at 588.111 kb on + strand at 588.116 kb on - strand at 588.128 kb on - strand at 588.128 kb on - strand at 588.128 kb on - strand at 588.129 kb on + strand at 588.130 kb on - strand at 588.130 kb on - strand at 588.130 kb on - strand at 588.131 kb on + strand at 588.131 kb on + strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.133 kb on + strand at 588.133 kb on + strand at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.137 kb on - strand, within MMP_RS03115 at 588.137 kb on - strand, within MMP_RS03115 at 588.138 kb on + strand, within MMP_RS03115 at 588.139 kb on - strand, within MMP_RS03115 at 588.140 kb on + strand, within MMP_RS03115 at 588.202 kb on - strand, within MMP_RS03115 at 588.203 kb on + strand, within MMP_RS03115 at 588.209 kb on + strand, within MMP_RS03115 at 588.220 kb on - strand, within MMP_RS03115 at 588.225 kb on + strand, within MMP_RS03115 at 588.253 kb on - strand, within MMP_RS03115 at 588.253 kb on - strand, within MMP_RS03115 at 588.254 kb on + strand, within MMP_RS03115 at 588.272 kb on + strand, within MMP_RS03115 at 588.289 kb on - strand, within MMP_RS03115 at 588.290 kb on + strand, within MMP_RS03115 at 588.341 kb on + strand, within MMP_RS03115 at 588.344 kb on - strand, within MMP_RS03115 at 588.493 kb on - strand, within MMP_RS03115 at 588.498 kb on + strand, within MMP_RS03115 at 588.498 kb on + strand, within MMP_RS03115 at 588.524 kb on - strand, within MMP_RS03115 at 588.524 kb on - strand, within MMP_RS03115 at 588.524 kb on - strand, within MMP_RS03115 at 588.525 kb on + strand, within MMP_RS03115 at 588.525 kb on + strand, within MMP_RS03115 at 588.525 kb on + strand, within MMP_RS03115 at 588.538 kb on + strand, within MMP_RS03115 at 588.554 kb on + strand, within MMP_RS03115 at 588.581 kb on + strand, within MMP_RS03115 at 588.689 kb on - strand at 588.712 kb on + strand at 588.717 kb on + strand at 588.727 kb on - strand at 588.728 kb on + strand at 588.880 kb on + strand, within MMP_RS03120 at 588.880 kb on + strand, within MMP_RS03120 at 588.888 kb on - strand, within MMP_RS03120 at 588.891 kb on - strand, within MMP_RS03120 at 588.938 kb on - strand at 588.993 kb on - strand at 589.069 kb on - strand, within MMP_RS03125 at 589.129 kb on - strand, within MMP_RS03125 at 589.284 kb on + strand, within MMP_RS03125 at 589.295 kb on - strand, within MMP_RS03125 at 589.322 kb on + strand, within MMP_RS03125 at 589.596 kb on + strand, within MMP_RS03125 at 589.683 kb on + strand at 589.736 kb on - strand at 589.737 kb on + strand at 589.737 kb on + strand at 589.738 kb on - strand at 589.738 kb on - strand at 589.738 kb on - strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.740 kb on - strand at 589.741 kb on + strand at 589.746 kb on + strand at 589.754 kb on + strand at 589.764 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.768 kb on + strand at 589.769 kb on - strand at 589.769 kb on - strand at 589.780 kb on - strand at 589.827 kb on + strand at 589.827 kb on + strand at 589.887 kb on + strand, within MMP_RS03130 at 589.889 kb on - strand, within MMP_RS03130 at 589.897 kb on + strand, within MMP_RS03130 at 590.016 kb on + strand, within MMP_RS03130 at 590.031 kb on - strand, within MMP_RS03130 at 590.055 kb on - strand, within MMP_RS03130 at 590.060 kb on - strand, within MMP_RS03130 at 590.100 kb on + strand, within MMP_RS03130 at 590.100 kb on + strand, within MMP_RS03130 at 590.103 kb on + strand, within MMP_RS03130 at 590.267 kb on - strand, within MMP_RS03130 at 590.277 kb on - strand, within MMP_RS03130 at 590.323 kb on - strand at 590.387 kb on - strand at 590.401 kb on + strand at 590.446 kb on - strand at 590.528 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete rubidium chloride 125 mM remove 587,920 - MMP_RS03110 0.84 -3.6 587,939 - MMP_RS03110 0.87 -0.1 587,961 + MMP_RS03110 0.90 -3.1 588,047 + +1.3 588,065 + +1.8 588,101 - +0.9 588,101 - +1.9 588,101 - +2.4 588,101 - -0.3 588,102 + -0.9 588,102 + -1.6 588,104 - +0.8 588,106 - +0.7 588,107 + +0.6 588,110 - +2.2 588,111 + +1.2 588,116 - -2.2 588,128 - +0.4 588,128 - +0.9 588,128 - +1.1 588,129 + +2.6 588,130 - +1.4 588,130 - +0.8 588,130 - -0.8 588,131 + +1.4 588,131 + -0.3 588,132 - +1.3 588,132 - -1.0 588,132 - +0.2 588,132 - +1.4 588,132 - +0.0 588,132 - -0.1 588,132 - +1.5 588,132 - +0.4 588,132 - +0.3 588,132 - -0.0 588,132 - +0.6 588,133 + -0.9 588,133 + -1.4 588,135 - MMP_RS03115 0.10 +1.1 588,135 - MMP_RS03115 0.10 +0.1 588,135 - MMP_RS03115 0.10 +0.3 588,135 - MMP_RS03115 0.10 +2.3 588,135 - MMP_RS03115 0.10 -0.0 588,135 - MMP_RS03115 0.10 +1.7 588,135 - MMP_RS03115 0.10 +2.3 588,135 - MMP_RS03115 0.10 -0.2 588,136 + MMP_RS03115 0.10 +1.1 588,136 + MMP_RS03115 0.10 +0.7 588,136 + MMP_RS03115 0.10 +0.1 588,136 + MMP_RS03115 0.10 +1.5 588,136 + MMP_RS03115 0.10 -1.0 588,136 + MMP_RS03115 0.10 +3.6 588,136 + MMP_RS03115 0.10 +0.1 588,136 + MMP_RS03115 0.10 +2.0 588,137 - MMP_RS03115 0.10 +0.7 588,137 - MMP_RS03115 0.10 +1.1 588,138 + MMP_RS03115 0.11 -0.2 588,139 - MMP_RS03115 0.11 +0.8 588,140 + MMP_RS03115 0.11 +0.8 588,202 - MMP_RS03115 0.20 -0.6 588,203 + MMP_RS03115 0.20 +0.8 588,209 + MMP_RS03115 0.21 -0.1 588,220 - MMP_RS03115 0.23 +0.6 588,225 + MMP_RS03115 0.24 +0.3 588,253 - MMP_RS03115 0.28 -0.9 588,253 - MMP_RS03115 0.28 +0.6 588,254 + MMP_RS03115 0.28 -0.4 588,272 + MMP_RS03115 0.31 -0.1 588,289 - MMP_RS03115 0.33 +0.0 588,290 + MMP_RS03115 0.34 +0.9 588,341 + MMP_RS03115 0.41 -0.1 588,344 - MMP_RS03115 0.42 +1.0 588,493 - MMP_RS03115 0.64 +1.5 588,498 + MMP_RS03115 0.65 -0.0 588,498 + MMP_RS03115 0.65 +1.5 588,524 - MMP_RS03115 0.69 +1.1 588,524 - MMP_RS03115 0.69 -1.0 588,524 - MMP_RS03115 0.69 +1.0 588,525 + MMP_RS03115 0.69 -0.2 588,525 + MMP_RS03115 0.69 +0.5 588,525 + MMP_RS03115 0.69 +1.0 588,538 + MMP_RS03115 0.71 -0.7 588,554 + MMP_RS03115 0.74 +3.1 588,581 + MMP_RS03115 0.78 +0.1 588,689 - +0.7 588,712 + +0.6 588,717 + -0.5 588,727 - +0.1 588,728 + +1.3 588,880 + MMP_RS03120 0.78 -0.9 588,880 + MMP_RS03120 0.78 -1.2 588,888 - MMP_RS03120 0.82 +0.4 588,891 - MMP_RS03120 0.84 -0.1 588,938 - +0.2 588,993 - -0.1 589,069 - MMP_RS03125 0.19 -0.3 589,129 - MMP_RS03125 0.27 -0.9 589,284 + MMP_RS03125 0.47 -0.4 589,295 - MMP_RS03125 0.48 -0.4 589,322 + MMP_RS03125 0.52 -1.1 589,596 + MMP_RS03125 0.86 -0.7 589,683 + -0.9 589,736 - -0.2 589,737 + -1.3 589,737 + +0.2 589,738 - +0.2 589,738 - -0.7 589,738 - +0.4 589,739 + -0.3 589,739 + -0.3 589,739 + -0.6 589,739 + -0.4 589,739 + -2.2 589,740 - -0.8 589,741 + -0.5 589,746 + -1.2 589,754 + -1.5 589,764 - -1.1 589,767 - -0.6 589,767 - -0.6 589,767 - -1.1 589,767 - +0.6 589,768 + -0.1 589,769 - -0.3 589,769 - +0.3 589,780 - +1.6 589,827 + +1.1 589,827 + +0.7 589,887 + MMP_RS03130 0.19 -0.8 589,889 - MMP_RS03130 0.19 +0.8 589,897 + MMP_RS03130 0.21 -0.1 590,016 + MMP_RS03130 0.42 -0.8 590,031 - MMP_RS03130 0.44 +1.5 590,055 - MMP_RS03130 0.49 -0.3 590,060 - MMP_RS03130 0.50 +0.7 590,100 + MMP_RS03130 0.57 -0.6 590,100 + MMP_RS03130 0.57 -0.2 590,103 + MMP_RS03130 0.57 -0.8 590,267 - MMP_RS03130 0.86 -0.4 590,277 - MMP_RS03130 0.88 +0.0 590,323 - -0.1 590,387 - -1.5 590,401 + -1.6 590,446 - -1.2 590,528 - -0.7
Or see this region's nucleotide sequence