Experiment: McCas_H2_complete rubidium chloride 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03095 and MMP_RS03100 are separated by 5 nucleotides MMP_RS03100 and MMP_RS03105 are separated by 261 nucleotides MMP_RS03105 and MMP_RS03110 are separated by 4 nucleotides MMP_RS03110 and MMP_RS03115 are separated by 36 nucleotides
MMP_RS03095: MMP_RS03095 - diphthine synthase, at 584,564 to 585,331
_RS03095
MMP_RS03100: MMP_RS03100 - hypothetical protein, at 585,337 to 585,537
_RS03100
MMP_RS03105: MMP_RS03105 - radical SAM protein, at 585,799 to 587,331
_RS03105
MMP_RS03110: MMP_RS03110 - glycosyltransferase family 2 protein, at 587,336 to 588,031
_RS03110
MMP_RS03115: MMP_RS03115 - hypothetical protein, at 588,068 to 588,727
_RS03115
Position (kb) 585
586
587
588 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 585.002 kb on - strand, within MMP_RS03095 at 585.071 kb on + strand, within MMP_RS03095 at 585.206 kb on - strand, within MMP_RS03095 at 585.210 kb on + strand, within MMP_RS03095 at 585.401 kb on + strand, within MMP_RS03100 at 585.509 kb on + strand, within MMP_RS03100 at 585.512 kb on - strand, within MMP_RS03100 at 585.512 kb on - strand, within MMP_RS03100 at 585.513 kb on + strand, within MMP_RS03100 at 585.514 kb on - strand, within MMP_RS03100 at 585.551 kb on - strand at 585.553 kb on - strand at 585.553 kb on - strand at 585.553 kb on - strand at 585.554 kb on + strand at 585.572 kb on - strand at 585.573 kb on + strand at 585.587 kb on - strand at 585.592 kb on + strand at 585.627 kb on + strand at 585.675 kb on + strand at 585.739 kb on - strand at 585.739 kb on - strand at 585.741 kb on - strand at 585.741 kb on - strand at 585.756 kb on - strand at 585.757 kb on + strand at 585.757 kb on + strand at 585.763 kb on - strand at 585.763 kb on - strand at 585.764 kb on + strand at 585.764 kb on + strand at 585.766 kb on + strand at 585.766 kb on + strand at 585.824 kb on + strand at 585.936 kb on - strand at 585.979 kb on - strand, within MMP_RS03105 at 585.980 kb on + strand, within MMP_RS03105 at 585.986 kb on - strand, within MMP_RS03105 at 585.987 kb on + strand, within MMP_RS03105 at 585.990 kb on - strand, within MMP_RS03105 at 586.117 kb on + strand, within MMP_RS03105 at 586.345 kb on + strand, within MMP_RS03105 at 586.378 kb on + strand, within MMP_RS03105 at 586.389 kb on - strand, within MMP_RS03105 at 586.797 kb on + strand, within MMP_RS03105 at 586.956 kb on + strand, within MMP_RS03105 at 586.964 kb on - strand, within MMP_RS03105 at 586.964 kb on - strand, within MMP_RS03105 at 586.965 kb on + strand, within MMP_RS03105 at 586.982 kb on - strand, within MMP_RS03105 at 586.999 kb on + strand, within MMP_RS03105 at 587.000 kb on - strand, within MMP_RS03105 at 587.001 kb on + strand, within MMP_RS03105 at 587.009 kb on - strand, within MMP_RS03105 at 587.009 kb on - strand, within MMP_RS03105 at 587.009 kb on - strand, within MMP_RS03105 at 587.031 kb on - strand, within MMP_RS03105 at 587.033 kb on - strand, within MMP_RS03105 at 587.040 kb on - strand, within MMP_RS03105 at 587.111 kb on - strand, within MMP_RS03105 at 587.157 kb on + strand, within MMP_RS03105 at 587.228 kb on - strand at 587.349 kb on + strand at 587.352 kb on + strand at 587.352 kb on + strand at 587.352 kb on + strand at 587.396 kb on + strand at 587.398 kb on - strand at 587.398 kb on - strand at 587.399 kb on + strand at 587.403 kb on - strand at 587.404 kb on + strand at 587.425 kb on + strand, within MMP_RS03110 at 587.435 kb on - strand, within MMP_RS03110 at 587.440 kb on + strand, within MMP_RS03110 at 587.487 kb on + strand, within MMP_RS03110 at 587.551 kb on - strand, within MMP_RS03110 at 587.573 kb on + strand, within MMP_RS03110 at 587.662 kb on - strand, within MMP_RS03110 at 587.662 kb on - strand, within MMP_RS03110 at 587.663 kb on + strand, within MMP_RS03110 at 587.732 kb on + strand, within MMP_RS03110 at 587.803 kb on + strand, within MMP_RS03110 at 587.864 kb on - strand, within MMP_RS03110 at 587.920 kb on - strand, within MMP_RS03110 at 587.939 kb on - strand, within MMP_RS03110 at 587.961 kb on + strand, within MMP_RS03110 at 588.047 kb on + strand at 588.065 kb on + strand at 588.101 kb on - strand at 588.101 kb on - strand at 588.101 kb on - strand at 588.101 kb on - strand at 588.102 kb on + strand at 588.102 kb on + strand at 588.104 kb on - strand at 588.106 kb on - strand at 588.107 kb on + strand at 588.110 kb on - strand at 588.111 kb on + strand at 588.116 kb on - strand at 588.128 kb on - strand at 588.128 kb on - strand at 588.128 kb on - strand at 588.129 kb on + strand at 588.130 kb on - strand at 588.130 kb on - strand at 588.130 kb on - strand at 588.131 kb on + strand at 588.131 kb on + strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.132 kb on - strand at 588.133 kb on + strand at 588.133 kb on + strand at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.135 kb on - strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.136 kb on + strand, within MMP_RS03115 at 588.137 kb on - strand, within MMP_RS03115 at 588.137 kb on - strand, within MMP_RS03115 at 588.138 kb on + strand, within MMP_RS03115 at 588.139 kb on - strand, within MMP_RS03115 at 588.140 kb on + strand, within MMP_RS03115 at 588.202 kb on - strand, within MMP_RS03115 at 588.203 kb on + strand, within MMP_RS03115 at 588.209 kb on + strand, within MMP_RS03115 at 588.220 kb on - strand, within MMP_RS03115 at 588.225 kb on + strand, within MMP_RS03115 at 588.253 kb on - strand, within MMP_RS03115 at 588.253 kb on - strand, within MMP_RS03115 at 588.254 kb on + strand, within MMP_RS03115 at 588.272 kb on + strand, within MMP_RS03115 at 588.289 kb on - strand, within MMP_RS03115 at 588.290 kb on + strand, within MMP_RS03115
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete rubidium chloride 125 mM remove 585,002 - MMP_RS03095 0.57 +0.4 585,071 + MMP_RS03095 0.66 -0.3 585,206 - MMP_RS03095 0.84 -0.5 585,210 + MMP_RS03095 0.84 -0.8 585,401 + MMP_RS03100 0.32 -0.6 585,509 + MMP_RS03100 0.86 +1.0 585,512 - MMP_RS03100 0.87 -0.9 585,512 - MMP_RS03100 0.87 +0.0 585,513 + MMP_RS03100 0.88 -0.6 585,514 - MMP_RS03100 0.88 +1.1 585,551 - +1.5 585,553 - +1.4 585,553 - +0.1 585,553 - +0.1 585,554 + -0.3 585,572 - +0.6 585,573 + -0.8 585,587 - -0.5 585,592 + -0.6 585,627 + -0.1 585,675 + -0.2 585,739 - -0.2 585,739 - -1.1 585,741 - -0.4 585,741 - +0.7 585,756 - +0.7 585,757 + +0.4 585,757 + +0.9 585,763 - -0.7 585,763 - +0.7 585,764 + +0.9 585,764 + -0.5 585,766 + -0.7 585,766 + -0.4 585,824 + -0.9 585,936 - +0.6 585,979 - MMP_RS03105 0.12 -0.6 585,980 + MMP_RS03105 0.12 -1.3 585,986 - MMP_RS03105 0.12 +0.6 585,987 + MMP_RS03105 0.12 -1.1 585,990 - MMP_RS03105 0.12 -0.2 586,117 + MMP_RS03105 0.21 -1.0 586,345 + MMP_RS03105 0.36 -2.3 586,378 + MMP_RS03105 0.38 -0.4 586,389 - MMP_RS03105 0.38 -0.1 586,797 + MMP_RS03105 0.65 -0.7 586,956 + MMP_RS03105 0.75 -1.5 586,964 - MMP_RS03105 0.76 +0.2 586,964 - MMP_RS03105 0.76 -0.4 586,965 + MMP_RS03105 0.76 +0.5 586,982 - MMP_RS03105 0.77 +0.9 586,999 + MMP_RS03105 0.78 -0.3 587,000 - MMP_RS03105 0.78 -0.8 587,001 + MMP_RS03105 0.78 -2.4 587,009 - MMP_RS03105 0.79 -0.7 587,009 - MMP_RS03105 0.79 -0.9 587,009 - MMP_RS03105 0.79 +0.4 587,031 - MMP_RS03105 0.80 -1.7 587,033 - MMP_RS03105 0.80 -0.1 587,040 - MMP_RS03105 0.81 -0.3 587,111 - MMP_RS03105 0.86 -1.4 587,157 + MMP_RS03105 0.89 -0.4 587,228 - +1.0 587,349 + +1.2 587,352 + -0.5 587,352 + +0.6 587,352 + +1.5 587,396 + -0.2 587,398 - +1.1 587,398 - +1.0 587,399 + +1.7 587,403 - +1.1 587,404 + -1.1 587,425 + MMP_RS03110 0.13 +0.2 587,435 - MMP_RS03110 0.14 +1.6 587,440 + MMP_RS03110 0.15 +0.1 587,487 + MMP_RS03110 0.22 -1.7 587,551 - MMP_RS03110 0.31 +2.3 587,573 + MMP_RS03110 0.34 -0.5 587,662 - MMP_RS03110 0.47 +0.0 587,662 - MMP_RS03110 0.47 +1.7 587,663 + MMP_RS03110 0.47 -0.9 587,732 + MMP_RS03110 0.57 +0.1 587,803 + MMP_RS03110 0.67 -0.5 587,864 - MMP_RS03110 0.76 -1.8 587,920 - MMP_RS03110 0.84 -3.6 587,939 - MMP_RS03110 0.87 -0.1 587,961 + MMP_RS03110 0.90 -3.1 588,047 + +1.3 588,065 + +1.8 588,101 - +0.9 588,101 - +1.9 588,101 - +2.4 588,101 - -0.3 588,102 + -0.9 588,102 + -1.6 588,104 - +0.8 588,106 - +0.7 588,107 + +0.6 588,110 - +2.2 588,111 + +1.2 588,116 - -2.2 588,128 - +0.4 588,128 - +0.9 588,128 - +1.1 588,129 + +2.6 588,130 - +1.4 588,130 - +0.8 588,130 - -0.8 588,131 + +1.4 588,131 + -0.3 588,132 - +1.3 588,132 - -1.0 588,132 - +0.2 588,132 - +1.4 588,132 - +0.0 588,132 - -0.1 588,132 - +1.5 588,132 - +0.4 588,132 - +0.3 588,132 - -0.0 588,132 - +0.6 588,133 + -0.9 588,133 + -1.4 588,135 - MMP_RS03115 0.10 +1.1 588,135 - MMP_RS03115 0.10 +0.1 588,135 - MMP_RS03115 0.10 +0.3 588,135 - MMP_RS03115 0.10 +2.3 588,135 - MMP_RS03115 0.10 -0.0 588,135 - MMP_RS03115 0.10 +1.7 588,135 - MMP_RS03115 0.10 +2.3 588,135 - MMP_RS03115 0.10 -0.2 588,136 + MMP_RS03115 0.10 +1.1 588,136 + MMP_RS03115 0.10 +0.7 588,136 + MMP_RS03115 0.10 +0.1 588,136 + MMP_RS03115 0.10 +1.5 588,136 + MMP_RS03115 0.10 -1.0 588,136 + MMP_RS03115 0.10 +3.6 588,136 + MMP_RS03115 0.10 +0.1 588,136 + MMP_RS03115 0.10 +2.0 588,137 - MMP_RS03115 0.10 +0.7 588,137 - MMP_RS03115 0.10 +1.1 588,138 + MMP_RS03115 0.11 -0.2 588,139 - MMP_RS03115 0.11 +0.8 588,140 + MMP_RS03115 0.11 +0.8 588,202 - MMP_RS03115 0.20 -0.6 588,203 + MMP_RS03115 0.20 +0.8 588,209 + MMP_RS03115 0.21 -0.1 588,220 - MMP_RS03115 0.23 +0.6 588,225 + MMP_RS03115 0.24 +0.3 588,253 - MMP_RS03115 0.28 -0.9 588,253 - MMP_RS03115 0.28 +0.6 588,254 + MMP_RS03115 0.28 -0.4 588,272 + MMP_RS03115 0.31 -0.1 588,289 - MMP_RS03115 0.33 +0.0 588,290 + MMP_RS03115 0.34 +0.9
Or see this region's nucleotide sequence