Experiment: McCas_H2_complete rubidium chloride 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01535 and MMP_RS01540 are separated by 9 nucleotides MMP_RS01540 and MMP_RS01545 are separated by 5 nucleotides MMP_RS01545 and MMP_RS01550 are separated by 56 nucleotides
MMP_RS01535: MMP_RS01535 - DHH family phosphoesterase, at 286,576 to 288,036
_RS01535
MMP_RS01540: MMP_RS01540 - DUF504 domain-containing protein, at 288,046 to 288,276
_RS01540
MMP_RS01545: MMP_RS01545 - CBS domain-containing protein, at 288,282 to 289,076
_RS01545
MMP_RS01550: MMP_RS01550 - DUF1624 domain-containing protein, at 289,133 to 289,852
_RS01550
Position (kb) 288
289 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 287.494 kb on - strand at 287.494 kb on - strand at 287.768 kb on - strand at 287.779 kb on - strand at 287.781 kb on - strand at 287.782 kb on + strand at 287.813 kb on - strand at 287.841 kb on + strand at 287.841 kb on + strand at 288.044 kb on - strand at 288.104 kb on + strand, within MMP_RS01540 at 288.111 kb on + strand, within MMP_RS01540 at 288.590 kb on - strand, within MMP_RS01545 at 289.001 kb on - strand at 289.082 kb on + strand at 289.167 kb on - strand at 289.178 kb on + strand at 289.206 kb on + strand, within MMP_RS01550
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete rubidium chloride 125 mM remove 287,494 - -3.4 287,494 - -0.6 287,768 - -3.0 287,779 - +0.2 287,781 - -3.7 287,782 + -4.1 287,813 - +0.8 287,841 + -3.0 287,841 + +0.4 288,044 - -1.6 288,104 + MMP_RS01540 0.25 -0.3 288,111 + MMP_RS01540 0.28 -0.7 288,590 - MMP_RS01545 0.39 -1.1 289,001 - -2.2 289,082 + -0.1 289,167 - -1.6 289,178 + +0.2 289,206 + MMP_RS01550 0.10 -0.0
Or see this region's nucleotide sequence