Experiment: McCas_H2_complete rubidium chloride 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00425 and MMP_RS00430 are separated by 69 nucleotides MMP_RS00430 and MMP_RS00435 overlap by 12 nucleotides MMP_RS00435 and MMP_RS00440 are separated by 228 nucleotides MMP_RS00440 and MMP_RS00445 are separated by 143 nucleotides
MMP_RS00425: MMP_RS00425 - argininosuccinate synthase, at 84,962 to 86,155
_RS00425
MMP_RS00430: MMP_RS00430 - hypothetical protein, at 86,225 to 86,581
_RS00430
MMP_RS00435: MMP_RS00435 - CPBP family intramembrane metalloprotease, at 86,570 to 87,379
_RS00435
MMP_RS00440: MMP_RS00440 - TRAM domain-containing protein, at 87,608 to 87,817
_RS00440
MMP_RS00445: MMP_RS00445 - 7-carboxy-7-deazaguanine synthase QueE, at 87,961 to 88,689
_RS00445
Position (kb) 86
87
88 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 86.476 kb on - strand, within MMP_RS00430 at 86.476 kb on - strand, within MMP_RS00430 at 86.478 kb on - strand, within MMP_RS00430 at 86.610 kb on + strand at 86.673 kb on + strand, within MMP_RS00435 at 86.679 kb on + strand, within MMP_RS00435 at 86.684 kb on - strand, within MMP_RS00435 at 86.734 kb on - strand, within MMP_RS00435 at 86.737 kb on + strand, within MMP_RS00435 at 86.789 kb on - strand, within MMP_RS00435 at 86.790 kb on + strand, within MMP_RS00435 at 86.851 kb on - strand, within MMP_RS00435 at 86.855 kb on - strand, within MMP_RS00435 at 86.855 kb on - strand, within MMP_RS00435 at 86.855 kb on - strand, within MMP_RS00435 at 86.855 kb on - strand, within MMP_RS00435 at 86.856 kb on + strand, within MMP_RS00435 at 86.902 kb on - strand, within MMP_RS00435 at 86.902 kb on - strand, within MMP_RS00435 at 86.940 kb on - strand, within MMP_RS00435 at 86.968 kb on - strand, within MMP_RS00435 at 86.987 kb on - strand, within MMP_RS00435 at 87.110 kb on - strand, within MMP_RS00435 at 87.113 kb on + strand, within MMP_RS00435 at 87.134 kb on - strand, within MMP_RS00435 at 87.218 kb on + strand, within MMP_RS00435 at 87.363 kb on - strand at 87.368 kb on - strand at 87.488 kb on - strand at 87.491 kb on + strand at 87.612 kb on + strand at 87.821 kb on - strand at 87.829 kb on - strand at 87.832 kb on - strand at 87.832 kb on - strand at 87.833 kb on + strand at 87.835 kb on - strand at 87.836 kb on + strand at 87.836 kb on + strand at 87.837 kb on - strand at 87.838 kb on + strand at 87.838 kb on + strand at 87.839 kb on - strand at 87.839 kb on - strand at 87.839 kb on - strand at 87.839 kb on - strand at 87.840 kb on + strand at 87.840 kb on + strand at 87.840 kb on + strand at 87.840 kb on + strand at 87.840 kb on + strand at 87.847 kb on - strand at 87.856 kb on - strand at 87.856 kb on - strand at 87.856 kb on - strand at 87.856 kb on - strand at 87.857 kb on + strand at 87.857 kb on + strand at 87.861 kb on - strand at 87.878 kb on - strand at 87.882 kb on - strand at 87.882 kb on - strand at 87.884 kb on - strand at 87.884 kb on - strand at 87.884 kb on - strand at 87.884 kb on - strand at 87.884 kb on - strand at 87.885 kb on + strand at 88.056 kb on - strand, within MMP_RS00445 at 88.057 kb on + strand, within MMP_RS00445 at 88.057 kb on + strand, within MMP_RS00445 at 88.057 kb on + strand, within MMP_RS00445 at 88.058 kb on - strand, within MMP_RS00445 at 88.058 kb on - strand, within MMP_RS00445 at 88.059 kb on + strand, within MMP_RS00445 at 88.059 kb on + strand, within MMP_RS00445 at 88.061 kb on + strand, within MMP_RS00445 at 88.061 kb on + strand, within MMP_RS00445 at 88.061 kb on + strand, within MMP_RS00445 at 88.135 kb on - strand, within MMP_RS00445 at 88.136 kb on + strand, within MMP_RS00445 at 88.231 kb on - strand, within MMP_RS00445 at 88.281 kb on - strand, within MMP_RS00445 at 88.347 kb on + strand, within MMP_RS00445
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete rubidium chloride 125 mM remove 86,476 - MMP_RS00430 0.70 +1.2 86,476 - MMP_RS00430 0.70 -1.2 86,478 - MMP_RS00430 0.71 -0.3 86,610 + +0.6 86,673 + MMP_RS00435 0.13 +0.2 86,679 + MMP_RS00435 0.13 +0.2 86,684 - MMP_RS00435 0.14 -0.5 86,734 - MMP_RS00435 0.20 +0.6 86,737 + MMP_RS00435 0.21 +0.4 86,789 - MMP_RS00435 0.27 +2.0 86,790 + MMP_RS00435 0.27 +0.5 86,851 - MMP_RS00435 0.35 +0.7 86,855 - MMP_RS00435 0.35 -0.3 86,855 - MMP_RS00435 0.35 +0.8 86,855 - MMP_RS00435 0.35 -0.5 86,855 - MMP_RS00435 0.35 +0.2 86,856 + MMP_RS00435 0.35 -0.0 86,902 - MMP_RS00435 0.41 +1.6 86,902 - MMP_RS00435 0.41 -0.1 86,940 - MMP_RS00435 0.46 -0.8 86,968 - MMP_RS00435 0.49 +0.7 86,987 - MMP_RS00435 0.51 +0.3 87,110 - MMP_RS00435 0.67 +0.6 87,113 + MMP_RS00435 0.67 +2.0 87,134 - MMP_RS00435 0.70 +0.6 87,218 + MMP_RS00435 0.80 -0.3 87,363 - -0.8 87,368 - -0.0 87,488 - +0.2 87,491 + +0.2 87,612 + -0.1 87,821 - -0.5 87,829 - -0.4 87,832 - -3.6 87,832 - -0.4 87,833 + -1.0 87,835 - -2.0 87,836 + -0.3 87,836 + -2.8 87,837 - -1.6 87,838 + -0.2 87,838 + -5.1 87,839 - +1.3 87,839 - -0.9 87,839 - -3.3 87,839 - -0.3 87,840 + +0.2 87,840 + -0.5 87,840 + -0.4 87,840 + -1.3 87,840 + -0.8 87,847 - -1.1 87,856 - -1.1 87,856 - -1.5 87,856 - -0.8 87,856 - -2.4 87,857 + -1.3 87,857 + -1.1 87,861 - -2.6 87,878 - -0.6 87,882 - -1.1 87,882 - +0.0 87,884 - -0.9 87,884 - -0.5 87,884 - +0.2 87,884 - -1.5 87,884 - +0.0 87,885 + -0.7 88,056 - MMP_RS00445 0.13 +0.2 88,057 + MMP_RS00445 0.13 +0.6 88,057 + MMP_RS00445 0.13 -0.2 88,057 + MMP_RS00445 0.13 -0.0 88,058 - MMP_RS00445 0.13 +0.5 88,058 - MMP_RS00445 0.13 +0.3 88,059 + MMP_RS00445 0.13 +0.4 88,059 + MMP_RS00445 0.13 +0.2 88,061 + MMP_RS00445 0.14 +0.0 88,061 + MMP_RS00445 0.14 +0.7 88,061 + MMP_RS00445 0.14 -2.0 88,135 - MMP_RS00445 0.24 -1.1 88,136 + MMP_RS00445 0.24 +0.3 88,231 - MMP_RS00445 0.37 -0.7 88,281 - MMP_RS00445 0.44 -0.0 88,347 + MMP_RS00445 0.53 -1.2
Or see this region's nucleotide sequence