Strain Fitness in Methanococcus maripaludis S2 around MMP_RS01050

Experiment: McCas_H2_complete silver chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS01045 and MMP_RS01050 are separated by 84 nucleotidesMMP_RS01050 and MMP_RS01055 are separated by 334 nucleotidesMMP_RS01055 and MMP_RS01060 are separated by 236 nucleotides MMP_RS01045: MMP_RS01045 - diphthamide biosynthesis enzyme Dph2, at 196,599 to 197,528 _RS01045 MMP_RS01050: MMP_RS01050 - tRNA-Leu, at 197,613 to 197,697 _RS01050 MMP_RS01055: MMP_RS01055 - hypothetical protein, at 198,032 to 198,409 _RS01055 MMP_RS01060: MMP_RS01060 - calcium/sodium antiporter, at 198,646 to 199,608 _RS01060 Position (kb) 197 198Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 196.781 kb on + strand, within MMP_RS01045at 196.817 kb on + strand, within MMP_RS01045at 196.892 kb on - strand, within MMP_RS01045at 196.959 kb on + strand, within MMP_RS01045at 196.998 kb on + strand, within MMP_RS01045at 196.998 kb on + strand, within MMP_RS01045at 197.096 kb on - strand, within MMP_RS01045at 197.209 kb on - strand, within MMP_RS01045at 197.209 kb on - strand, within MMP_RS01045at 197.322 kb on - strand, within MMP_RS01045at 197.323 kb on + strand, within MMP_RS01045at 197.325 kb on + strand, within MMP_RS01045at 197.443 kb on - strandat 197.443 kb on - strandat 197.470 kb on + strandat 197.516 kb on - strandat 197.524 kb on + strandat 197.811 kb on - strandat 197.856 kb on - strandat 197.894 kb on - strandat 198.086 kb on - strand, within MMP_RS01055at 198.086 kb on - strand, within MMP_RS01055at 198.170 kb on + strand, within MMP_RS01055at 198.406 kb on + strandat 198.406 kb on + strandat 198.411 kb on - strandat 198.411 kb on - strandat 198.412 kb on + strandat 198.444 kb on - strandat 198.569 kb on - strandat 198.605 kb on - strandat 198.640 kb on + strandat 198.644 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_H2_complete silver chloride 0.5 mM
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196,781 + MMP_RS01045 0.20 -0.7
196,817 + MMP_RS01045 0.23 -2.6
196,892 - MMP_RS01045 0.32 -1.1
196,959 + MMP_RS01045 0.39 -2.5
196,998 + MMP_RS01045 0.43 +1.3
196,998 + MMP_RS01045 0.43 +4.7
197,096 - MMP_RS01045 0.53 -1.1
197,209 - MMP_RS01045 0.66 -0.6
197,209 - MMP_RS01045 0.66 +1.2
197,322 - MMP_RS01045 0.78 -1.7
197,323 + MMP_RS01045 0.78 +0.4
197,325 + MMP_RS01045 0.78 -1.0
197,443 - -0.7
197,443 - -0.4
197,470 + -1.1
197,516 - -1.6
197,524 + +0.5
197,811 - -0.9
197,856 - +1.8
197,894 - -1.9
198,086 - MMP_RS01055 0.14 +0.8
198,086 - MMP_RS01055 0.14 +0.4
198,170 + MMP_RS01055 0.37 +2.8
198,406 + -1.4
198,406 + +0.2
198,411 - +1.4
198,411 - +0.1
198,412 + +0.3
198,444 - +1.4
198,569 - +0.7
198,605 - -5.2
198,640 + +0.5
198,644 - +0.1

Or see this region's nucleotide sequence