Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08215 and MMP_RS08220 are separated by 15 nucleotides MMP_RS08220 and MMP_RS08225 are separated by 30 nucleotides MMP_RS08225 and MMP_RS08230 are separated by 27 nucleotides MMP_RS08230 and MMP_RS08235 are separated by 106 nucleotides
MMP_RS08215: MMP_RS08215 - DUF483 domain-containing protein, at 1,546,728 to 1,547,282
_RS08215
MMP_RS08220: MMP_RS08220 - TrkA family potassium uptake protein, at 1,547,298 to 1,547,963
_RS08220
MMP_RS08225: MMP_RS08225 - RimK family alpha-L-glutamate ligase, at 1,547,994 to 1,548,800
_RS08225
MMP_RS08230: MMP_RS08230 - 50S ribosomal protein L11 methyltransferase, at 1,548,828 to 1,549,610
_RS08230
MMP_RS08235: MMP_RS08235 - hypothetical protein, at 1,549,717 to 1,549,944
_RS08235
Position (kb)
1547
1548
1549 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1547.033 kb on + strand, within MMP_RS08215 at 1547.033 kb on + strand, within MMP_RS08215 at 1547.064 kb on + strand, within MMP_RS08215 at 1547.374 kb on - strand at 1547.500 kb on - strand at 1547.501 kb on + strand at 1547.996 kb on + strand at 1548.000 kb on - strand at 1548.009 kb on + strand at 1548.017 kb on + strand at 1548.062 kb on - strand at 1548.088 kb on + strand, within MMP_RS08225 at 1548.310 kb on - strand, within MMP_RS08225 at 1548.311 kb on + strand, within MMP_RS08225 at 1548.311 kb on + strand, within MMP_RS08225 at 1548.418 kb on - strand, within MMP_RS08225 at 1548.418 kb on - strand, within MMP_RS08225 at 1548.418 kb on - strand, within MMP_RS08225 at 1548.418 kb on - strand, within MMP_RS08225 at 1548.419 kb on + strand, within MMP_RS08225 at 1548.419 kb on + strand, within MMP_RS08225 at 1548.419 kb on + strand, within MMP_RS08225 at 1548.419 kb on + strand, within MMP_RS08225 at 1548.420 kb on - strand, within MMP_RS08225 at 1548.420 kb on - strand, within MMP_RS08225 at 1548.430 kb on + strand, within MMP_RS08225 at 1548.532 kb on + strand, within MMP_RS08225 at 1549.088 kb on - strand, within MMP_RS08230 at 1549.144 kb on - strand, within MMP_RS08230 at 1549.145 kb on + strand, within MMP_RS08230 at 1549.287 kb on - strand, within MMP_RS08230 at 1549.303 kb on + strand, within MMP_RS08230 at 1549.333 kb on - strand, within MMP_RS08230 at 1549.351 kb on + strand, within MMP_RS08230 at 1549.369 kb on - strand, within MMP_RS08230 at 1549.370 kb on + strand, within MMP_RS08230 at 1549.398 kb on + strand, within MMP_RS08230 at 1549.538 kb on - strand at 1549.563 kb on - strand at 1549.576 kb on - strand at 1549.576 kb on - strand at 1549.577 kb on + strand at 1549.577 kb on + strand at 1549.656 kb on + strand at 1549.657 kb on - strand at 1549.657 kb on - strand at 1549.658 kb on + strand at 1549.658 kb on + strand at 1549.658 kb on + strand at 1549.658 kb on + strand at 1549.658 kb on + strand at 1549.714 kb on - strand at 1549.750 kb on + strand, within MMP_RS08235
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 1,547,033 + MMP_RS08215 0.55 +1.9 1,547,033 + MMP_RS08215 0.55 -0.5 1,547,064 + MMP_RS08215 0.61 +0.4 1,547,374 - -2.4 1,547,500 - -3.4 1,547,501 + +0.3 1,547,996 + +1.2 1,548,000 - +0.4 1,548,009 + -1.3 1,548,017 + -0.8 1,548,062 - +1.0 1,548,088 + MMP_RS08225 0.12 +3.1 1,548,310 - MMP_RS08225 0.39 +1.2 1,548,311 + MMP_RS08225 0.39 -0.4 1,548,311 + MMP_RS08225 0.39 -1.6 1,548,418 - MMP_RS08225 0.53 +0.1 1,548,418 - MMP_RS08225 0.53 -0.2 1,548,418 - MMP_RS08225 0.53 -1.5 1,548,418 - MMP_RS08225 0.53 -0.4 1,548,419 + MMP_RS08225 0.53 +0.5 1,548,419 + MMP_RS08225 0.53 +0.9 1,548,419 + MMP_RS08225 0.53 -1.1 1,548,419 + MMP_RS08225 0.53 -2.8 1,548,420 - MMP_RS08225 0.53 +0.3 1,548,420 - MMP_RS08225 0.53 +0.3 1,548,430 + MMP_RS08225 0.54 +3.9 1,548,532 + MMP_RS08225 0.67 -0.0 1,549,088 - MMP_RS08230 0.33 +0.7 1,549,144 - MMP_RS08230 0.40 -0.2 1,549,145 + MMP_RS08230 0.40 -0.0 1,549,287 - MMP_RS08230 0.59 -0.8 1,549,303 + MMP_RS08230 0.61 -1.1 1,549,333 - MMP_RS08230 0.64 -1.2 1,549,351 + MMP_RS08230 0.67 -2.1 1,549,369 - MMP_RS08230 0.69 +2.2 1,549,370 + MMP_RS08230 0.69 -0.6 1,549,398 + MMP_RS08230 0.73 -0.5 1,549,538 - +0.2 1,549,563 - -0.0 1,549,576 - +1.2 1,549,576 - +0.2 1,549,577 + +0.6 1,549,577 + +0.0 1,549,656 + -1.5 1,549,657 - +0.4 1,549,657 - -0.4 1,549,658 + +1.8 1,549,658 + +0.4 1,549,658 + +0.1 1,549,658 + -0.4 1,549,658 + +1.7 1,549,714 - -0.1 1,549,750 + MMP_RS08235 0.14 +1.1
Or see this region's nucleotide sequence