Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07960 and MMP_RS07965 are separated by 21 nucleotides MMP_RS07965 and MMP_RS07970 overlap by 4 nucleotides MMP_RS07970 and MMP_RS07975 are separated by 138 nucleotides
MMP_RS07960: MMP_RS07960 - sugar phosphate isomerase/epimerase, at 1,502,975 to 1,503,781
_RS07960
MMP_RS07965: MMP_RS07965 - NADPH-dependent F420 reductase, at 1,503,803 to 1,504,474
_RS07965
MMP_RS07970: MMP_RS07970 - hypothetical protein, at 1,504,471 to 1,504,692
_RS07970
MMP_RS07975: MMP_RS07975 - signal recognition particle protein Srp54, at 1,504,831 to 1,506,183
_RS07975
Position (kb)
1504
1505 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1503.494 kb on + strand, within MMP_RS07960 at 1503.680 kb on + strand, within MMP_RS07960 at 1503.683 kb on + strand, within MMP_RS07960 at 1503.686 kb on - strand, within MMP_RS07960 at 1503.686 kb on - strand, within MMP_RS07960 at 1503.687 kb on + strand, within MMP_RS07960 at 1503.759 kb on + strand at 1504.038 kb on + strand at 1504.092 kb on - strand at 1504.465 kb on + strand at 1504.472 kb on - strand at 1504.548 kb on - strand, within MMP_RS07970 at 1504.577 kb on + strand, within MMP_RS07970 at 1504.683 kb on - strand at 1504.724 kb on + strand at 1504.755 kb on + strand at 1504.781 kb on + strand at 1504.817 kb on + strand at 1504.817 kb on + strand at 1504.841 kb on - strand at 1504.841 kb on - strand at 1504.842 kb on + strand at 1505.287 kb on - strand at 1505.287 kb on - strand at 1505.341 kb on - strand at 1505.342 kb on + strand at 1505.368 kb on - strand at 1505.437 kb on - strand at 1505.630 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 1,503,494 + MMP_RS07960 0.64 +0.7 1,503,680 + MMP_RS07960 0.87 +0.6 1,503,683 + MMP_RS07960 0.88 -0.7 1,503,686 - MMP_RS07960 0.88 +0.3 1,503,686 - MMP_RS07960 0.88 +0.6 1,503,687 + MMP_RS07960 0.88 -0.3 1,503,759 + +0.1 1,504,038 + -1.5 1,504,092 - -4.1 1,504,465 + +1.7 1,504,472 - +0.5 1,504,548 - MMP_RS07970 0.35 +0.4 1,504,577 + MMP_RS07970 0.48 +2.3 1,504,683 - -2.7 1,504,724 + +0.8 1,504,755 + +1.6 1,504,781 + +0.7 1,504,817 + +0.7 1,504,817 + +1.1 1,504,841 - -1.9 1,504,841 - -0.1 1,504,842 + +1.4 1,505,287 - -2.7 1,505,287 - -4.7 1,505,341 - -1.9 1,505,342 + -3.8 1,505,368 - -5.0 1,505,437 - -3.7 1,505,630 + -4.4
Or see this region's nucleotide sequence