Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06180 and MMP_RS06185 are separated by 250 nucleotides MMP_RS06185 and MMP_RS06190 are separated by 9 nucleotides MMP_RS06190 and MMP_RS06195 are separated by 7 nucleotides
MMP_RS06180: MMP_RS06180 - diaminopimelate decarboxylase, at 1,186,534 to 1,187,844
_RS06180
MMP_RS06185: MMP_RS06185 - thermonuclease family protein, at 1,188,095 to 1,188,652
_RS06185
MMP_RS06190: MMP_RS06190 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,188,662 to 1,189,045
_RS06190
MMP_RS06195: MMP_RS06195 - cobalt-precorrin-5B (C(1))-methyltransferase CbiD, at 1,189,053 to 1,190,150
_RS06195
Position (kb)
1188
1189 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1187.302 kb on - strand at 1187.361 kb on + strand at 1187.537 kb on + strand at 1187.722 kb on - strand at 1187.782 kb on + strand at 1187.820 kb on - strand at 1187.848 kb on + strand at 1187.878 kb on - strand at 1187.884 kb on - strand at 1187.903 kb on - strand at 1187.908 kb on - strand at 1187.918 kb on + strand at 1187.956 kb on - strand at 1187.959 kb on - strand at 1187.960 kb on + strand at 1188.089 kb on + strand at 1188.094 kb on + strand at 1188.185 kb on + strand, within MMP_RS06185 at 1188.373 kb on + strand, within MMP_RS06185 at 1188.373 kb on + strand, within MMP_RS06185 at 1188.403 kb on - strand, within MMP_RS06185 at 1188.404 kb on + strand, within MMP_RS06185 at 1188.557 kb on - strand, within MMP_RS06185 at 1188.576 kb on - strand, within MMP_RS06185 at 1188.580 kb on - strand, within MMP_RS06185 at 1188.580 kb on - strand, within MMP_RS06185 at 1188.598 kb on - strand at 1188.598 kb on - strand at 1188.599 kb on + strand at 1188.653 kb on + strand at 1188.655 kb on - strand at 1188.739 kb on + strand, within MMP_RS06190 at 1188.739 kb on + strand, within MMP_RS06190 at 1188.739 kb on + strand, within MMP_RS06190 at 1188.740 kb on - strand, within MMP_RS06190 at 1188.740 kb on - strand, within MMP_RS06190 at 1188.741 kb on + strand, within MMP_RS06190 at 1188.777 kb on - strand, within MMP_RS06190 at 1188.786 kb on + strand, within MMP_RS06190 at 1188.788 kb on + strand, within MMP_RS06190 at 1189.071 kb on - strand at 1189.072 kb on + strand at 1189.074 kb on + strand at 1189.085 kb on - strand at 1189.090 kb on + strand at 1189.094 kb on - strand at 1189.095 kb on + strand at 1189.095 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 1,187,302 - -1.3 1,187,361 + -1.8 1,187,537 + -3.6 1,187,722 - +0.3 1,187,782 + -1.0 1,187,820 - -1.5 1,187,848 + +1.4 1,187,878 - +0.9 1,187,884 - -1.3 1,187,903 - -0.1 1,187,908 - -0.5 1,187,918 + -1.5 1,187,956 - +1.0 1,187,959 - +1.6 1,187,960 + +1.5 1,188,089 + +0.1 1,188,094 + +2.2 1,188,185 + MMP_RS06185 0.16 +1.7 1,188,373 + MMP_RS06185 0.50 +1.3 1,188,373 + MMP_RS06185 0.50 +2.0 1,188,403 - MMP_RS06185 0.55 +0.5 1,188,404 + MMP_RS06185 0.55 +1.4 1,188,557 - MMP_RS06185 0.83 +0.7 1,188,576 - MMP_RS06185 0.86 +1.4 1,188,580 - MMP_RS06185 0.87 -1.6 1,188,580 - MMP_RS06185 0.87 +1.7 1,188,598 - +1.0 1,188,598 - -0.2 1,188,599 + +0.4 1,188,653 + +0.5 1,188,655 - -0.9 1,188,739 + MMP_RS06190 0.20 +1.1 1,188,739 + MMP_RS06190 0.20 +0.5 1,188,739 + MMP_RS06190 0.20 +0.2 1,188,740 - MMP_RS06190 0.20 +0.5 1,188,740 - MMP_RS06190 0.20 +0.4 1,188,741 + MMP_RS06190 0.21 +0.3 1,188,777 - MMP_RS06190 0.30 +0.2 1,188,786 + MMP_RS06190 0.32 +1.5 1,188,788 + MMP_RS06190 0.33 +0.3 1,189,071 - -4.7 1,189,072 + +0.0 1,189,074 + -3.7 1,189,085 - -3.0 1,189,090 + -2.4 1,189,094 - -4.2 1,189,095 + +0.4 1,189,095 + -4.9
Or see this region's nucleotide sequence