Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04255 and MMP_RS04260 are separated by 21 nucleotides MMP_RS04260 and MMP_RS04265 are separated by 10 nucleotides MMP_RS04265 and MMP_RS04270 are separated by 13 nucleotides
MMP_RS04255: MMP_RS04255 - coenzyme F420 hydrogenase subunit beta, at 811,639 to 812,487
_RS04255
MMP_RS04260: MMP_RS04260 - coenzyme F420 hydrogenase subunit gamma, at 812,509 to 813,237
_RS04260
MMP_RS04265: MMP_RS04265 - coenzyme F420-reducing hydrogenase FrhD protein, at 813,248 to 813,748
_RS04265
MMP_RS04270: MMP_RS04270 - coenzyme F420 hydrogenase subunit alpha, at 813,762 to 814,994
_RS04270
Position (kb)
812
813
814 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 811.514 kb on + strand at 811.525 kb on + strand at 811.552 kb on - strand at 811.553 kb on + strand at 811.560 kb on - strand at 811.601 kb on - strand at 811.607 kb on - strand at 811.607 kb on - strand at 811.726 kb on - strand, within MMP_RS04255 at 811.872 kb on - strand, within MMP_RS04255 at 811.925 kb on - strand, within MMP_RS04255 at 811.925 kb on - strand, within MMP_RS04255 at 811.926 kb on + strand, within MMP_RS04255 at 811.935 kb on - strand, within MMP_RS04255 at 812.048 kb on + strand, within MMP_RS04255 at 812.101 kb on + strand, within MMP_RS04255 at 812.124 kb on - strand, within MMP_RS04255 at 812.213 kb on - strand, within MMP_RS04255 at 812.242 kb on - strand, within MMP_RS04255 at 812.374 kb on - strand, within MMP_RS04255 at 812.457 kb on - strand at 812.457 kb on - strand at 812.458 kb on + strand at 812.466 kb on - strand at 812.468 kb on - strand at 812.468 kb on - strand at 812.487 kb on - strand at 812.488 kb on + strand at 812.506 kb on - strand at 812.507 kb on + strand at 812.527 kb on - strand at 812.528 kb on + strand at 812.632 kb on - strand, within MMP_RS04260 at 812.699 kb on + strand, within MMP_RS04260 at 812.913 kb on - strand, within MMP_RS04260 at 812.913 kb on - strand, within MMP_RS04260 at 813.010 kb on - strand, within MMP_RS04260 at 813.133 kb on - strand, within MMP_RS04260 at 813.569 kb on - strand, within MMP_RS04265 at 813.570 kb on + strand, within MMP_RS04265 at 813.696 kb on + strand, within MMP_RS04265 at 813.764 kb on + strand at 813.768 kb on - strand at 813.769 kb on + strand at 813.813 kb on - strand at 813.943 kb on - strand, within MMP_RS04270 at 813.947 kb on - strand, within MMP_RS04270 at 814.144 kb on + strand, within MMP_RS04270 at 814.225 kb on - strand, within MMP_RS04270
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 811,514 + +0.0 811,525 + -2.0 811,552 - -1.6 811,553 + +0.4 811,560 - -1.0 811,601 - +0.4 811,607 - -0.5 811,607 - +0.8 811,726 - MMP_RS04255 0.10 -0.9 811,872 - MMP_RS04255 0.27 -1.9 811,925 - MMP_RS04255 0.34 -1.7 811,925 - MMP_RS04255 0.34 -1.6 811,926 + MMP_RS04255 0.34 +0.4 811,935 - MMP_RS04255 0.35 -2.5 812,048 + MMP_RS04255 0.48 -0.7 812,101 + MMP_RS04255 0.54 -1.3 812,124 - MMP_RS04255 0.57 -2.2 812,213 - MMP_RS04255 0.68 +0.9 812,242 - MMP_RS04255 0.71 +0.2 812,374 - MMP_RS04255 0.87 -0.0 812,457 - +1.1 812,457 - +0.1 812,458 + +0.3 812,466 - +1.0 812,468 - -0.3 812,468 - +3.1 812,487 - -0.1 812,488 + -0.2 812,506 - -1.9 812,507 + +0.6 812,527 - +0.1 812,528 + -0.4 812,632 - MMP_RS04260 0.17 +1.6 812,699 + MMP_RS04260 0.26 +0.1 812,913 - MMP_RS04260 0.55 +0.7 812,913 - MMP_RS04260 0.55 +1.7 813,010 - MMP_RS04260 0.69 +1.1 813,133 - MMP_RS04260 0.86 +0.8 813,569 - MMP_RS04265 0.64 -0.7 813,570 + MMP_RS04265 0.64 +0.3 813,696 + MMP_RS04265 0.89 +0.1 813,764 + +0.7 813,768 - -1.0 813,769 + +0.4 813,813 - +0.2 813,943 - MMP_RS04270 0.15 +1.9 813,947 - MMP_RS04270 0.15 +0.9 814,144 + MMP_RS04270 0.31 +0.2 814,225 - MMP_RS04270 0.38 -0.0
Or see this region's nucleotide sequence