Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03300 and MMP_RS03305 are separated by 168 nucleotides MMP_RS03305 and MMP_RS03310 are separated by 177 nucleotides MMP_RS03310 and MMP_RS08960 are separated by 14 nucleotides MMP_RS08960 and MMP_RS03315 are separated by 114 nucleotides MMP_RS03315 and MMP_RS03320 are separated by 111 nucleotides
MMP_RS03300: MMP_RS03300 - TOBE domain-containing protein, at 620,371 to 620,577
_RS03300
MMP_RS03305: MMP_RS03305 - winged helix-turn-helix domain-containing protein, at 620,746 to 621,144
_RS03305
MMP_RS03310: MMP_RS03310 - ferrous iron transport protein B, at 621,322 to 623,265
_RS03310
MMP_RS08960: MMP_RS08960 - hypothetical protein, at 623,280 to 623,417
_RS08960
MMP_RS03315: MMP_RS03315 - ferrous iron transport protein A, at 623,532 to 623,738
_RS03315
MMP_RS03320: MMP_RS03320 - metal-dependent transcriptional regulator, at 623,850 to 624,254
_RS03320
Position (kb)
621
622
623
624 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 620.336 kb on - strand at 620.368 kb on - strand at 620.369 kb on + strand at 620.369 kb on + strand at 620.370 kb on - strand at 620.382 kb on - strand at 620.546 kb on - strand, within MMP_RS03300 at 620.642 kb on - strand at 620.643 kb on + strand at 620.645 kb on + strand at 620.662 kb on + strand at 620.664 kb on + strand at 620.679 kb on + strand at 620.682 kb on - strand at 620.682 kb on - strand at 620.683 kb on + strand at 620.685 kb on + strand at 620.685 kb on + strand at 620.686 kb on - strand at 620.692 kb on - strand at 620.716 kb on + strand at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.835 kb on + strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.891 kb on - strand, within MMP_RS03305 at 620.930 kb on + strand, within MMP_RS03305 at 621.127 kb on + strand at 621.142 kb on - strand at 621.167 kb on + strand at 621.196 kb on - strand at 621.239 kb on - strand at 621.239 kb on - strand at 621.267 kb on - strand at 621.268 kb on + strand at 621.268 kb on + strand at 621.271 kb on - strand at 621.273 kb on - strand at 621.323 kb on - strand at 621.334 kb on - strand at 621.334 kb on - strand at 621.350 kb on - strand at 621.350 kb on - strand at 621.355 kb on - strand at 621.390 kb on + strand at 621.855 kb on - strand at 621.894 kb on - strand at 622.326 kb on - strand at 622.331 kb on + strand at 622.385 kb on + strand at 622.838 kb on + strand at 623.036 kb on - strand at 623.247 kb on - strand at 623.421 kb on - strand at 623.462 kb on - strand at 623.473 kb on + strand at 623.497 kb on + strand at 623.504 kb on + strand at 623.517 kb on - strand at 623.693 kb on - strand, within MMP_RS03315 at 623.702 kb on - strand, within MMP_RS03315 at 623.704 kb on - strand, within MMP_RS03315 at 623.755 kb on - strand at 623.755 kb on - strand at 623.756 kb on + strand at 623.822 kb on - strand at 623.823 kb on + strand at 623.823 kb on + strand at 623.824 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.827 kb on + strand at 623.828 kb on - strand at 623.828 kb on - strand at 623.828 kb on - strand at 623.887 kb on - strand at 623.897 kb on - strand, within MMP_RS03320 at 623.901 kb on + strand, within MMP_RS03320 at 623.909 kb on - strand, within MMP_RS03320 at 624.012 kb on - strand, within MMP_RS03320 at 624.023 kb on - strand, within MMP_RS03320
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 620,336 - +1.7 620,368 - +0.2 620,369 + -1.5 620,369 + +1.2 620,370 - +0.5 620,382 - -3.5 620,546 - MMP_RS03300 0.85 -4.5 620,642 - +1.0 620,643 + -0.4 620,645 + -0.4 620,662 + +0.3 620,664 + +1.0 620,679 + +2.1 620,682 - +2.9 620,682 - +1.3 620,683 + +1.1 620,685 + +2.4 620,685 + +0.9 620,686 - +3.0 620,692 - +3.6 620,716 + +2.4 620,793 + MMP_RS03305 0.12 +4.3 620,793 + MMP_RS03305 0.12 +3.7 620,793 + MMP_RS03305 0.12 +2.5 620,793 + MMP_RS03305 0.12 +1.9 620,835 + MMP_RS03305 0.22 +3.2 620,859 - MMP_RS03305 0.28 +2.6 620,859 - MMP_RS03305 0.28 +4.0 620,859 - MMP_RS03305 0.28 +2.1 620,891 - MMP_RS03305 0.36 +1.7 620,930 + MMP_RS03305 0.46 +3.8 621,127 + +1.6 621,142 - +0.6 621,167 + +0.1 621,196 - -0.9 621,239 - +0.9 621,239 - +1.0 621,267 - +0.2 621,268 + +0.3 621,268 + +0.6 621,271 - +1.3 621,273 - +0.5 621,323 - +0.6 621,334 - +0.5 621,334 - -5.1 621,350 - -5.4 621,350 - -0.3 621,355 - -4.1 621,390 + -1.1 621,855 - -1.7 621,894 - -0.5 622,326 - -1.9 622,331 + -4.5 622,385 + -2.6 622,838 + -0.9 623,036 - -4.1 623,247 - -1.1 623,421 - +0.8 623,462 - +0.7 623,473 + +0.7 623,497 + +1.4 623,504 + -0.9 623,517 - +1.0 623,693 - MMP_RS03315 0.78 +0.3 623,702 - MMP_RS03315 0.82 -2.0 623,704 - MMP_RS03315 0.83 -4.5 623,755 - -1.9 623,755 - -4.4 623,756 + -3.9 623,822 - -4.7 623,823 + -3.4 623,823 + -1.7 623,824 - -2.0 623,826 - +0.5 623,826 - +1.3 623,826 - +2.0 623,826 - +0.1 623,826 - -3.4 623,826 - +0.8 623,827 + +1.2 623,828 - -2.7 623,828 - -4.5 623,828 - -1.4 623,887 - +1.0 623,897 - MMP_RS03320 0.12 -1.0 623,901 + MMP_RS03320 0.13 -0.8 623,909 - MMP_RS03320 0.15 -1.1 624,012 - MMP_RS03320 0.40 +1.3 624,023 - MMP_RS03320 0.43 +0.4
Or see this region's nucleotide sequence