Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02120 and MMP_RS02125 are separated by 41 nucleotides MMP_RS02125 and MMP_RS02130 are separated by 10 nucleotides MMP_RS02130 and MMP_RS02135 are separated by 9 nucleotides MMP_RS02135 and MMP_RS02140 are separated by 35 nucleotides
MMP_RS02120: MMP_RS02120 - alanine--tRNA ligase, at 394,599 to 397,277
_RS02120
MMP_RS02125: MMP_RS02125 - pantoate kinase, at 397,319 to 398,191
_RS02125
MMP_RS02130: MMP_RS02130 - zinc metalloprotease HtpX, at 398,202 to 399,059
_RS02130
MMP_RS02135: MMP_RS02135 - DUF5612 domain-containing protein, at 399,069 to 399,722
_RS02135
MMP_RS02140: MMP_RS02140 - methionine synthase, at 399,758 to 400,696
_RS02140
Position (kb) 398
399
400 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 397.309 kb on + strand at 397.336 kb on - strand at 397.361 kb on + strand at 397.870 kb on - strand at 397.871 kb on + strand at 397.872 kb on - strand at 397.873 kb on + strand at 397.900 kb on + strand at 397.998 kb on - strand at 397.998 kb on - strand at 398.136 kb on - strand at 398.190 kb on + strand at 398.211 kb on + strand at 398.268 kb on - strand at 398.269 kb on + strand at 398.271 kb on + strand at 398.271 kb on + strand at 398.271 kb on + strand at 398.300 kb on + strand, within MMP_RS02130 at 398.323 kb on - strand, within MMP_RS02130 at 398.324 kb on + strand, within MMP_RS02130 at 398.351 kb on + strand, within MMP_RS02130 at 398.364 kb on - strand, within MMP_RS02130 at 398.365 kb on + strand, within MMP_RS02130 at 398.365 kb on + strand, within MMP_RS02130 at 398.378 kb on + strand, within MMP_RS02130 at 398.522 kb on + strand, within MMP_RS02130 at 398.535 kb on + strand, within MMP_RS02130 at 398.602 kb on - strand, within MMP_RS02130 at 398.603 kb on + strand, within MMP_RS02130 at 398.617 kb on - strand, within MMP_RS02130 at 398.617 kb on - strand, within MMP_RS02130 at 398.618 kb on + strand, within MMP_RS02130 at 398.632 kb on - strand, within MMP_RS02130 at 398.661 kb on - strand, within MMP_RS02130 at 398.661 kb on - strand, within MMP_RS02130 at 398.662 kb on + strand, within MMP_RS02130 at 398.856 kb on - strand, within MMP_RS02130 at 399.089 kb on + strand at 399.119 kb on - strand at 399.158 kb on + strand, within MMP_RS02135 at 399.162 kb on - strand, within MMP_RS02135 at 399.162 kb on - strand, within MMP_RS02135 at 399.163 kb on + strand, within MMP_RS02135 at 399.192 kb on + strand, within MMP_RS02135 at 399.207 kb on + strand, within MMP_RS02135 at 399.423 kb on + strand, within MMP_RS02135 at 399.587 kb on + strand, within MMP_RS02135 at 399.694 kb on - strand at 399.695 kb on + strand at 399.789 kb on - strand at 399.850 kb on - strand at 399.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 397,309 + -2.1 397,336 - -1.3 397,361 + -3.6 397,870 - -1.7 397,871 + -0.7 397,872 - -4.8 397,873 + -5.2 397,900 + +0.1 397,998 - -3.4 397,998 - -2.6 398,136 - -4.2 398,190 + +0.3 398,211 + -1.1 398,268 - +0.7 398,269 + +0.7 398,271 + +1.4 398,271 + -0.0 398,271 + +0.2 398,300 + MMP_RS02130 0.11 -0.4 398,323 - MMP_RS02130 0.14 +1.3 398,324 + MMP_RS02130 0.14 -0.4 398,351 + MMP_RS02130 0.17 +0.1 398,364 - MMP_RS02130 0.19 +0.5 398,365 + MMP_RS02130 0.19 -3.8 398,365 + MMP_RS02130 0.19 -1.2 398,378 + MMP_RS02130 0.21 +0.7 398,522 + MMP_RS02130 0.37 -2.9 398,535 + MMP_RS02130 0.39 -1.5 398,602 - MMP_RS02130 0.47 -2.7 398,603 + MMP_RS02130 0.47 -0.5 398,617 - MMP_RS02130 0.48 -4.3 398,617 - MMP_RS02130 0.48 -2.7 398,618 + MMP_RS02130 0.48 +0.7 398,632 - MMP_RS02130 0.50 -3.2 398,661 - MMP_RS02130 0.53 -5.0 398,661 - MMP_RS02130 0.53 -4.7 398,662 + MMP_RS02130 0.54 +1.9 398,856 - MMP_RS02130 0.76 +0.3 399,089 + +2.0 399,119 - -5.3 399,158 + MMP_RS02135 0.14 -2.0 399,162 - MMP_RS02135 0.14 -2.9 399,162 - MMP_RS02135 0.14 -1.5 399,163 + MMP_RS02135 0.14 +1.4 399,192 + MMP_RS02135 0.19 -1.1 399,207 + MMP_RS02135 0.21 -4.7 399,423 + MMP_RS02135 0.54 -3.8 399,587 + MMP_RS02135 0.79 -0.5 399,694 - -4.7 399,695 + -4.7 399,789 - -3.9 399,850 - -2.0 399,948 - -0.9
Or see this region's nucleotide sequence