Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01740 and MMP_RS01745 are separated by 135 nucleotides MMP_RS01745 and MMP_RS01750 are separated by 109 nucleotides MMP_RS01750 and MMP_RS01755 are separated by 14 nucleotides
MMP_RS01740: MMP_RS01740 - methionine--tRNA ligase, at 322,398 to 324,389
_RS01740
MMP_RS01745: MMP_RS01745 - AMP phosphorylase, at 324,525 to 326,042
_RS01745
MMP_RS01750: MMP_RS01750 - DUF63 family protein, at 326,152 to 326,958
_RS01750
MMP_RS01755: MMP_RS01755 - DUF2097 domain-containing protein, at 326,973 to 327,263
_RS01755
Position (kb) 324
325
326
327 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 323.802 kb on - strand at 323.842 kb on - strand at 323.945 kb on + strand at 323.945 kb on + strand at 323.948 kb on - strand at 323.970 kb on + strand at 324.212 kb on + strand at 324.220 kb on + strand at 324.332 kb on - strand at 324.337 kb on - strand at 324.337 kb on - strand at 324.390 kb on - strand at 324.406 kb on - strand at 324.459 kb on - strand at 324.468 kb on - strand at 324.485 kb on - strand at 324.550 kb on - strand at 324.637 kb on - strand at 324.767 kb on + strand, within MMP_RS01745 at 324.918 kb on - strand, within MMP_RS01745 at 324.920 kb on - strand, within MMP_RS01745 at 325.025 kb on - strand, within MMP_RS01745 at 325.072 kb on - strand, within MMP_RS01745 at 325.469 kb on + strand, within MMP_RS01745 at 325.661 kb on + strand, within MMP_RS01745 at 325.929 kb on - strand at 325.972 kb on - strand at 325.972 kb on - strand at 326.019 kb on + strand at 326.058 kb on - strand at 326.060 kb on - strand at 326.060 kb on - strand at 326.061 kb on + strand at 326.061 kb on + strand at 326.065 kb on + strand at 326.081 kb on + strand at 326.087 kb on + strand at 326.116 kb on + strand at 326.131 kb on + strand at 326.194 kb on - strand at 326.195 kb on + strand at 326.227 kb on + strand at 326.268 kb on + strand, within MMP_RS01750 at 326.338 kb on + strand, within MMP_RS01750 at 326.445 kb on - strand, within MMP_RS01750 at 326.445 kb on - strand, within MMP_RS01750 at 326.449 kb on - strand, within MMP_RS01750 at 326.450 kb on + strand, within MMP_RS01750 at 326.501 kb on + strand, within MMP_RS01750 at 326.501 kb on + strand, within MMP_RS01750 at 326.505 kb on + strand, within MMP_RS01750 at 326.585 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.603 kb on - strand, within MMP_RS01750 at 326.613 kb on + strand, within MMP_RS01750 at 326.617 kb on - strand, within MMP_RS01750 at 326.620 kb on - strand, within MMP_RS01750 at 326.620 kb on - strand, within MMP_RS01750 at 326.621 kb on + strand, within MMP_RS01750 at 326.633 kb on + strand, within MMP_RS01750 at 326.837 kb on - strand, within MMP_RS01750 at 326.881 kb on + strand at 327.036 kb on - strand, within MMP_RS01755
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 323,802 - -2.3 323,842 - -2.0 323,945 + -2.5 323,945 + -2.3 323,948 - -2.0 323,970 + -0.4 324,212 + -4.4 324,220 + -3.3 324,332 - -4.7 324,337 - -0.2 324,337 - -0.4 324,390 - -1.3 324,406 - +0.7 324,459 - -1.1 324,468 - +0.5 324,485 - -0.0 324,550 - -0.3 324,637 - -1.9 324,767 + MMP_RS01745 0.16 -0.0 324,918 - MMP_RS01745 0.26 +0.4 324,920 - MMP_RS01745 0.26 -0.7 325,025 - MMP_RS01745 0.33 -3.3 325,072 - MMP_RS01745 0.36 -3.6 325,469 + MMP_RS01745 0.62 -1.5 325,661 + MMP_RS01745 0.75 -1.6 325,929 - -1.4 325,972 - -1.0 325,972 - -2.3 326,019 + +0.8 326,058 - -0.8 326,060 - -0.3 326,060 - +1.7 326,061 + +0.8 326,061 + +0.2 326,065 + +1.1 326,081 + -0.1 326,087 + -2.2 326,116 + -0.7 326,131 + -2.3 326,194 - -1.6 326,195 + +0.5 326,227 + +2.1 326,268 + MMP_RS01750 0.14 +1.1 326,338 + MMP_RS01750 0.23 +0.9 326,445 - MMP_RS01750 0.36 -0.1 326,445 - MMP_RS01750 0.36 +1.6 326,449 - MMP_RS01750 0.37 -4.4 326,450 + MMP_RS01750 0.37 -0.3 326,501 + MMP_RS01750 0.43 -0.6 326,501 + MMP_RS01750 0.43 -0.1 326,505 + MMP_RS01750 0.44 +1.5 326,585 - MMP_RS01750 0.54 -0.5 326,590 - MMP_RS01750 0.54 +0.9 326,590 - MMP_RS01750 0.54 -0.2 326,590 - MMP_RS01750 0.54 -0.3 326,590 - MMP_RS01750 0.54 -0.1 326,590 - MMP_RS01750 0.54 -1.1 326,591 + MMP_RS01750 0.54 +0.7 326,591 + MMP_RS01750 0.54 +1.1 326,591 + MMP_RS01750 0.54 +0.2 326,603 - MMP_RS01750 0.56 +0.6 326,613 + MMP_RS01750 0.57 -0.3 326,617 - MMP_RS01750 0.58 -0.2 326,620 - MMP_RS01750 0.58 -1.4 326,620 - MMP_RS01750 0.58 -0.5 326,621 + MMP_RS01750 0.58 +2.4 326,633 + MMP_RS01750 0.60 -0.7 326,837 - MMP_RS01750 0.85 -1.6 326,881 + +0.8 327,036 - MMP_RS01755 0.22 -1.1
Or see this region's nucleotide sequence