Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00315 and MMP_RS00320 overlap by 4 nucleotides MMP_RS00320 and MMP_RS00325 are separated by 13 nucleotides MMP_RS00325 and MMP_RS00330 are separated by 107 nucleotides
MMP_RS00315: MMP_RS00315 - Nif3-like dinuclear metal center hexameric protein, at 68,156 to 68,896
_RS00315
MMP_RS00320: MMP_RS00320 - class I SAM-dependent methyltransferase, at 68,893 to 69,501
_RS00320
MMP_RS00325: MMP_RS00325 - RNA-binding protein, at 69,515 to 69,994
_RS00325
MMP_RS00330: MMP_RS00330 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 70,102 to 71,181
_RS00330
Position (kb) 69
70 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 68.613 kb on - strand, within MMP_RS00315 at 68.711 kb on - strand, within MMP_RS00315 at 68.711 kb on - strand, within MMP_RS00315 at 68.754 kb on + strand, within MMP_RS00315 at 69.287 kb on + strand, within MMP_RS00320 at 69.302 kb on + strand, within MMP_RS00320 at 69.446 kb on - strand at 69.447 kb on + strand at 69.448 kb on - strand at 69.454 kb on + strand at 69.749 kb on - strand, within MMP_RS00325 at 69.750 kb on + strand, within MMP_RS00325 at 70.059 kb on - strand at 70.103 kb on - strand at 70.104 kb on + strand at 70.576 kb on + strand at 70.576 kb on + strand at 70.623 kb on - strand at 70.849 kb on + strand at 70.906 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 68,613 - MMP_RS00315 0.62 +0.1 68,711 - MMP_RS00315 0.75 -3.3 68,711 - MMP_RS00315 0.75 +0.4 68,754 + MMP_RS00315 0.81 -1.6 69,287 + MMP_RS00320 0.65 -0.2 69,302 + MMP_RS00320 0.67 +1.4 69,446 - +0.4 69,447 + -1.8 69,448 - -1.5 69,454 + -1.5 69,749 - MMP_RS00325 0.49 -0.7 69,750 + MMP_RS00325 0.49 -2.2 70,059 - -0.9 70,103 - +0.8 70,104 + +0.5 70,576 + -0.1 70,576 + -0.7 70,623 - -0.6 70,849 + -1.4 70,906 + +0.3
Or see this region's nucleotide sequence