Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00115 and MMP_RS00120 are separated by 72 nucleotides MMP_RS00120 and MMP_RS00125 are separated by 29 nucleotides MMP_RS00125 and MMP_RS00130 are separated by 91 nucleotides MMP_RS00130 and MMP_RS00135 are separated by 116 nucleotides
MMP_RS00115: MMP_RS00115 - radical SAM protein, at 26,673 to 27,725
_RS00115
MMP_RS00120: MMP_RS00120 - LysR family transcriptional regulator, at 27,798 to 28,640
_RS00120
MMP_RS00125: MMP_RS00125 - DUF2798 domain-containing protein, at 28,670 to 28,897
_RS00125
MMP_RS00130: MMP_RS00130 - nickel-responsive transcriptional regulator NikR, at 28,989 to 29,414
_RS00130
MMP_RS00135: MMP_RS00135 - DUF2121 domain-containing protein, at 29,531 to 30,406
_RS00135
Position (kb) 28
29 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 27.725 kb on - strand at 27.728 kb on - strand at 27.739 kb on + strand at 27.764 kb on - strand at 27.765 kb on + strand at 27.823 kb on - strand at 27.824 kb on + strand at 27.840 kb on + strand at 27.842 kb on + strand at 27.846 kb on - strand at 27.846 kb on - strand at 27.847 kb on + strand at 27.847 kb on + strand at 27.848 kb on - strand at 28.059 kb on - strand, within MMP_RS00120 at 28.060 kb on + strand, within MMP_RS00120 at 28.071 kb on - strand, within MMP_RS00120 at 28.115 kb on - strand, within MMP_RS00120 at 28.118 kb on + strand, within MMP_RS00120 at 28.332 kb on - strand, within MMP_RS00120 at 28.609 kb on - strand at 28.657 kb on + strand at 28.659 kb on - strand at 28.660 kb on + strand at 28.683 kb on + strand at 28.829 kb on - strand, within MMP_RS00125 at 28.900 kb on + strand at 28.900 kb on + strand at 28.924 kb on - strand at 29.300 kb on - strand, within MMP_RS00130 at 29.390 kb on - strand at 29.390 kb on - strand at 29.390 kb on - strand at 29.417 kb on - strand at 29.417 kb on - strand at 29.418 kb on + strand at 29.453 kb on + strand at 29.459 kb on - strand at 29.516 kb on + strand at 29.746 kb on + strand, within MMP_RS00135 at 29.770 kb on + strand, within MMP_RS00135 at 29.770 kb on + strand, within MMP_RS00135 at 29.807 kb on - strand, within MMP_RS00135 at 29.807 kb on - strand, within MMP_RS00135
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 27,725 - -1.9 27,728 - -5.0 27,739 + -2.3 27,764 - -1.5 27,765 + -0.1 27,823 - -2.6 27,824 + -1.2 27,840 + -1.9 27,842 + -0.3 27,846 - +0.2 27,846 - +0.1 27,847 + -2.0 27,847 + -0.3 27,848 - -0.8 28,059 - MMP_RS00120 0.31 +0.3 28,060 + MMP_RS00120 0.31 -0.9 28,071 - MMP_RS00120 0.32 +2.9 28,115 - MMP_RS00120 0.38 -0.2 28,118 + MMP_RS00120 0.38 -0.0 28,332 - MMP_RS00120 0.63 -0.9 28,609 - -1.6 28,657 + +0.1 28,659 - -0.2 28,660 + +0.8 28,683 + +2.0 28,829 - MMP_RS00125 0.70 -1.2 28,900 + -1.1 28,900 + +0.5 28,924 - +2.3 29,300 - MMP_RS00130 0.73 -0.3 29,390 - +0.7 29,390 - +1.2 29,390 - +1.1 29,417 - +1.0 29,417 - +0.3 29,418 + +2.0 29,453 + -0.3 29,459 - +0.3 29,516 + +1.6 29,746 + MMP_RS00135 0.25 -1.1 29,770 + MMP_RS00135 0.27 -0.1 29,770 + MMP_RS00135 0.27 -0.2 29,807 - MMP_RS00135 0.32 +0.4 29,807 - MMP_RS00135 0.32 +1.2
Or see this region's nucleotide sequence