Experiment: McCas_H2_complete Irgasan 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04715 and MMP_RS04720 are separated by 14 nucleotides MMP_RS04720 and MMP_RS04725 are separated by 38 nucleotides MMP_RS04725 and MMP_RS04730 are separated by 43 nucleotides MMP_RS04730 and MMP_RS04735 are separated by 75 nucleotides MMP_RS04735 and MMP_RS04740 are separated by 41 nucleotides
MMP_RS04715: MMP_RS04715 - CBS domain-containing protein, at 901,511 to 901,891
_RS04715
MMP_RS04720: MMP_RS04720 - DedA family protein, at 901,906 to 902,388
_RS04720
MMP_RS04725: MMP_RS04725 - hypothetical protein, at 902,427 to 902,855
_RS04725
MMP_RS04730: MMP_RS04730 - flavodoxin family protein, at 902,899 to 903,480
_RS04730
MMP_RS04735: MMP_RS04735 - tautomerase family protein, at 903,556 to 903,750
_RS04735
MMP_RS04740: MMP_RS04740 - DUF473 domain-containing protein, at 903,792 to 904,169
_RS04740
Position (kb)
902
903 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 901.453 kb on - strand at 901.470 kb on - strand at 901.470 kb on - strand at 901.474 kb on - strand at 901.474 kb on - strand at 901.475 kb on + strand at 901.476 kb on - strand at 901.476 kb on - strand at 901.477 kb on + strand at 901.477 kb on + strand at 901.478 kb on - strand at 901.478 kb on - strand at 901.479 kb on + strand at 901.479 kb on + strand at 901.481 kb on - strand at 901.511 kb on + strand at 901.582 kb on - strand, within MMP_RS04715 at 901.583 kb on + strand, within MMP_RS04715 at 901.583 kb on + strand, within MMP_RS04715 at 901.598 kb on + strand, within MMP_RS04715 at 901.620 kb on - strand, within MMP_RS04715 at 901.626 kb on - strand, within MMP_RS04715 at 901.627 kb on + strand, within MMP_RS04715 at 901.636 kb on - strand, within MMP_RS04715 at 901.648 kb on - strand, within MMP_RS04715 at 901.649 kb on + strand, within MMP_RS04715 at 901.650 kb on - strand, within MMP_RS04715 at 901.702 kb on + strand, within MMP_RS04715 at 901.710 kb on - strand, within MMP_RS04715 at 901.710 kb on - strand, within MMP_RS04715 at 901.729 kb on - strand, within MMP_RS04715 at 901.729 kb on - strand, within MMP_RS04715 at 901.740 kb on - strand, within MMP_RS04715 at 901.750 kb on + strand, within MMP_RS04715 at 901.931 kb on + strand at 902.011 kb on + strand, within MMP_RS04720 at 902.012 kb on - strand, within MMP_RS04720 at 902.016 kb on - strand, within MMP_RS04720 at 902.017 kb on + strand, within MMP_RS04720 at 902.017 kb on + strand, within MMP_RS04720 at 902.337 kb on + strand, within MMP_RS04720 at 902.341 kb on - strand at 902.366 kb on - strand at 902.391 kb on - strand at 902.426 kb on + strand at 902.482 kb on + strand, within MMP_RS04725 at 902.482 kb on + strand, within MMP_RS04725 at 902.482 kb on + strand, within MMP_RS04725 at 902.552 kb on - strand, within MMP_RS04725 at 902.557 kb on + strand, within MMP_RS04725 at 902.575 kb on - strand, within MMP_RS04725 at 902.575 kb on - strand, within MMP_RS04725 at 902.575 kb on - strand, within MMP_RS04725 at 902.643 kb on - strand, within MMP_RS04725 at 902.669 kb on - strand, within MMP_RS04725 at 902.691 kb on - strand, within MMP_RS04725 at 902.692 kb on + strand, within MMP_RS04725 at 902.696 kb on - strand, within MMP_RS04725 at 902.696 kb on - strand, within MMP_RS04725 at 902.696 kb on - strand, within MMP_RS04725 at 902.742 kb on + strand, within MMP_RS04725 at 902.758 kb on + strand, within MMP_RS04725 at 902.758 kb on + strand, within MMP_RS04725 at 902.817 kb on - strand at 902.873 kb on - strand at 902.873 kb on - strand at 902.897 kb on - strand at 902.897 kb on - strand at 902.897 kb on - strand at 902.898 kb on + strand at 902.898 kb on + strand at 902.900 kb on - strand at 902.902 kb on - strand at 902.903 kb on + strand at 902.903 kb on + strand at 902.943 kb on - strand at 903.018 kb on + strand, within MMP_RS04730 at 903.088 kb on - strand, within MMP_RS04730 at 903.094 kb on + strand, within MMP_RS04730 at 903.160 kb on + strand, within MMP_RS04730 at 903.171 kb on - strand, within MMP_RS04730 at 903.171 kb on - strand, within MMP_RS04730 at 903.228 kb on + strand, within MMP_RS04730 at 903.243 kb on - strand, within MMP_RS04730 at 903.244 kb on + strand, within MMP_RS04730 at 903.247 kb on - strand, within MMP_RS04730 at 903.248 kb on + strand, within MMP_RS04730 at 903.248 kb on + strand, within MMP_RS04730 at 903.248 kb on + strand, within MMP_RS04730 at 903.248 kb on + strand, within MMP_RS04730 at 903.250 kb on + strand, within MMP_RS04730 at 903.295 kb on - strand, within MMP_RS04730 at 903.301 kb on + strand, within MMP_RS04730 at 903.422 kb on + strand at 903.501 kb on - strand at 903.501 kb on - strand at 903.501 kb on - strand at 903.502 kb on + strand at 903.502 kb on + strand at 903.502 kb on + strand at 903.542 kb on - strand at 903.542 kb on - strand at 903.578 kb on + strand, within MMP_RS04735 at 903.632 kb on + strand, within MMP_RS04735 at 903.661 kb on - strand, within MMP_RS04735 at 903.661 kb on - strand, within MMP_RS04735 at 903.662 kb on + strand, within MMP_RS04735 at 903.793 kb on - strand at 903.794 kb on + strand at 903.804 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Irgasan 0.025 mM remove 901,453 - -5.2 901,470 - -3.9 901,470 - -2.6 901,474 - -3.9 901,474 - +0.1 901,475 + -1.7 901,476 - -3.9 901,476 - -5.1 901,477 + -4.1 901,477 + -2.3 901,478 - -0.9 901,478 - -1.6 901,479 + -3.4 901,479 + -0.3 901,481 - -0.6 901,511 + +1.9 901,582 - MMP_RS04715 0.19 -1.8 901,583 + MMP_RS04715 0.19 -0.8 901,583 + MMP_RS04715 0.19 -0.0 901,598 + MMP_RS04715 0.23 -1.3 901,620 - MMP_RS04715 0.29 -1.1 901,626 - MMP_RS04715 0.30 -1.4 901,627 + MMP_RS04715 0.30 +0.4 901,636 - MMP_RS04715 0.33 +0.1 901,648 - MMP_RS04715 0.36 +0.1 901,649 + MMP_RS04715 0.36 -0.4 901,650 - MMP_RS04715 0.36 -0.3 901,702 + MMP_RS04715 0.50 +0.4 901,710 - MMP_RS04715 0.52 -0.5 901,710 - MMP_RS04715 0.52 -1.5 901,729 - MMP_RS04715 0.57 -1.2 901,729 - MMP_RS04715 0.57 -0.3 901,740 - MMP_RS04715 0.60 +0.3 901,750 + MMP_RS04715 0.63 +1.6 901,931 + -1.8 902,011 + MMP_RS04720 0.22 -2.0 902,012 - MMP_RS04720 0.22 -3.4 902,016 - MMP_RS04720 0.23 -0.8 902,017 + MMP_RS04720 0.23 -3.6 902,017 + MMP_RS04720 0.23 -4.2 902,337 + MMP_RS04720 0.89 -0.6 902,341 - -1.0 902,366 - -1.1 902,391 - +0.0 902,426 + -0.1 902,482 + MMP_RS04725 0.13 -0.8 902,482 + MMP_RS04725 0.13 +0.1 902,482 + MMP_RS04725 0.13 +0.7 902,552 - MMP_RS04725 0.29 -1.2 902,557 + MMP_RS04725 0.30 +1.2 902,575 - MMP_RS04725 0.34 +0.6 902,575 - MMP_RS04725 0.34 -1.2 902,575 - MMP_RS04725 0.34 -0.5 902,643 - MMP_RS04725 0.50 +0.0 902,669 - MMP_RS04725 0.56 -2.0 902,691 - MMP_RS04725 0.62 -0.7 902,692 + MMP_RS04725 0.62 -2.6 902,696 - MMP_RS04725 0.63 -2.2 902,696 - MMP_RS04725 0.63 +1.1 902,696 - MMP_RS04725 0.63 +1.7 902,742 + MMP_RS04725 0.73 +0.8 902,758 + MMP_RS04725 0.77 -1.1 902,758 + MMP_RS04725 0.77 -1.1 902,817 - -1.7 902,873 - -0.6 902,873 - +0.2 902,897 - -2.2 902,897 - -0.5 902,897 - +1.8 902,898 + +0.7 902,898 + +0.6 902,900 - -0.4 902,902 - +1.6 902,903 + +0.4 902,903 + +3.3 902,943 - -0.6 903,018 + MMP_RS04730 0.20 +1.2 903,088 - MMP_RS04730 0.32 +0.5 903,094 + MMP_RS04730 0.34 -1.2 903,160 + MMP_RS04730 0.45 -1.5 903,171 - MMP_RS04730 0.47 +1.5 903,171 - MMP_RS04730 0.47 +0.1 903,228 + MMP_RS04730 0.57 +0.5 903,243 - MMP_RS04730 0.59 -0.8 903,244 + MMP_RS04730 0.59 -0.5 903,247 - MMP_RS04730 0.60 +2.2 903,248 + MMP_RS04730 0.60 -0.7 903,248 + MMP_RS04730 0.60 +1.2 903,248 + MMP_RS04730 0.60 +0.4 903,248 + MMP_RS04730 0.60 -1.6 903,250 + MMP_RS04730 0.60 -0.2 903,295 - MMP_RS04730 0.68 +1.1 903,301 + MMP_RS04730 0.69 -0.7 903,422 + -1.7 903,501 - +0.5 903,501 - +1.0 903,501 - -0.3 903,502 + -1.1 903,502 + +0.7 903,502 + +1.4 903,542 - +0.9 903,542 - -0.4 903,578 + MMP_RS04735 0.11 +0.8 903,632 + MMP_RS04735 0.39 +0.7 903,661 - MMP_RS04735 0.54 +1.7 903,661 - MMP_RS04735 0.54 +0.6 903,662 + MMP_RS04735 0.54 -0.4 903,793 - +0.7 903,794 + -0.4 903,804 - -0.4
Or see this region's nucleotide sequence