Experiment: McCas_H2_complete Irgasan 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04270 and MMP_RS04275 are separated by 358 nucleotides MMP_RS04275 and MMP_RS04280 are separated by 16 nucleotides MMP_RS04280 and MMP_RS04285 are separated by 21 nucleotides
MMP_RS04270: MMP_RS04270 - coenzyme F420 hydrogenase subunit alpha, at 813,762 to 814,994
_RS04270
MMP_RS04275: MMP_RS04275 - hydrogenase iron-sulfur subunit, at 815,353 to 815,775
_RS04275
MMP_RS04280: MMP_RS04280 - coenzyme F420-non-reducing hydrogenase subunit gamma, at 815,792 to 816,694
_RS04280
MMP_RS04285: MMP_RS04285 - Ni/Fe hydrogenase subunit alpha, at 816,716 to 818,131
_RS04285
Position (kb) 815
816
817 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 814.890 kb on - strand at 814.894 kb on - strand at 814.895 kb on + strand at 815.283 kb on + strand at 815.407 kb on - strand, within MMP_RS04275 at 815.407 kb on - strand, within MMP_RS04275 at 815.422 kb on + strand, within MMP_RS04275 at 815.436 kb on - strand, within MMP_RS04275 at 815.446 kb on - strand, within MMP_RS04275 at 815.460 kb on + strand, within MMP_RS04275 at 815.465 kb on - strand, within MMP_RS04275 at 815.465 kb on - strand, within MMP_RS04275 at 815.510 kb on - strand, within MMP_RS04275 at 815.521 kb on + strand, within MMP_RS04275 at 815.621 kb on + strand, within MMP_RS04275 at 815.706 kb on - strand, within MMP_RS04275 at 815.770 kb on + strand at 815.778 kb on + strand at 815.778 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.807 kb on + strand at 815.807 kb on + strand at 815.841 kb on - strand at 815.841 kb on - strand at 815.842 kb on + strand at 815.856 kb on - strand at 815.856 kb on - strand at 816.082 kb on + strand, within MMP_RS04280 at 816.121 kb on - strand, within MMP_RS04280 at 816.268 kb on + strand, within MMP_RS04280 at 816.310 kb on - strand, within MMP_RS04280 at 816.449 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.496 kb on - strand, within MMP_RS04280 at 816.520 kb on - strand, within MMP_RS04280 at 816.542 kb on + strand, within MMP_RS04280 at 816.570 kb on + strand, within MMP_RS04280 at 816.692 kb on - strand at 816.951 kb on - strand, within MMP_RS04285 at 817.135 kb on + strand, within MMP_RS04285 at 817.417 kb on - strand, within MMP_RS04285 at 817.417 kb on - strand, within MMP_RS04285 at 817.422 kb on + strand, within MMP_RS04285 at 817.527 kb on + strand, within MMP_RS04285 at 817.640 kb on - strand, within MMP_RS04285 at 817.644 kb on + strand, within MMP_RS04285
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Irgasan 0.025 mM remove 814,890 - +0.2 814,894 - -2.8 814,895 + +0.3 815,283 + -0.4 815,407 - MMP_RS04275 0.13 -0.8 815,407 - MMP_RS04275 0.13 +0.3 815,422 + MMP_RS04275 0.16 +3.1 815,436 - MMP_RS04275 0.20 +0.8 815,446 - MMP_RS04275 0.22 +0.7 815,460 + MMP_RS04275 0.25 +0.2 815,465 - MMP_RS04275 0.26 +0.2 815,465 - MMP_RS04275 0.26 +0.2 815,510 - MMP_RS04275 0.37 -0.9 815,521 + MMP_RS04275 0.40 -0.3 815,621 + MMP_RS04275 0.63 -1.9 815,706 - MMP_RS04275 0.83 -0.9 815,770 + -2.9 815,778 + -0.1 815,778 + -0.3 815,781 + -0.3 815,781 + +1.1 815,781 + -0.6 815,781 + -1.0 815,807 + +0.8 815,807 + +0.5 815,841 - -1.4 815,841 - -1.0 815,842 + -0.2 815,856 - +0.2 815,856 - +0.0 816,082 + MMP_RS04280 0.32 +0.2 816,121 - MMP_RS04280 0.36 -0.6 816,268 + MMP_RS04280 0.53 +0.9 816,310 - MMP_RS04280 0.57 -0.2 816,449 + MMP_RS04280 0.73 -0.2 816,472 + MMP_RS04280 0.75 +0.1 816,472 + MMP_RS04280 0.75 -1.4 816,472 + MMP_RS04280 0.75 +0.8 816,496 - MMP_RS04280 0.78 -1.1 816,520 - MMP_RS04280 0.81 +0.2 816,542 + MMP_RS04280 0.83 -1.1 816,570 + MMP_RS04280 0.86 -0.9 816,692 - -0.2 816,951 - MMP_RS04285 0.17 -0.1 817,135 + MMP_RS04285 0.30 +0.4 817,417 - MMP_RS04285 0.50 +0.2 817,417 - MMP_RS04285 0.50 -0.5 817,422 + MMP_RS04285 0.50 -0.4 817,527 + MMP_RS04285 0.57 -0.4 817,640 - MMP_RS04285 0.65 -0.7 817,644 + MMP_RS04285 0.66 +0.8
Or see this region's nucleotide sequence