Experiment: McCas_H2_complete Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04185 and MMP_RS04190 are separated by 87 nucleotides MMP_RS04190 and MMP_RS04195 are separated by 217 nucleotides MMP_RS04195 and MMP_RS04200 are separated by 30 nucleotides MMP_RS04200 and MMP_RS04205 are separated by 130 nucleotides
MMP_RS04185: MMP_RS04185 - aldo/keto reductase, at 796,127 to 797,239
_RS04185
MMP_RS04190: MMP_RS04190 - hypothetical protein, at 797,327 to 797,842
_RS04190
MMP_RS04195: MMP_RS04195 - flavodoxin, at 798,060 to 798,596
_RS04195
MMP_RS04200: MMP_RS04200 - DUF111 family protein, at 798,627 to 799,346
_RS04200
MMP_RS04205: MMP_RS04205 - carboxymuconolactone decarboxylase family protein, at 799,477 to 800,229
_RS04205
Position (kb)
798
799 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 797.099 kb on - strand, within MMP_RS04185 at 797.100 kb on + strand, within MMP_RS04185 at 797.172 kb on - strand at 797.273 kb on - strand at 797.305 kb on + strand at 797.361 kb on - strand at 797.362 kb on + strand at 797.362 kb on + strand at 797.362 kb on + strand at 797.365 kb on + strand at 797.403 kb on + strand, within MMP_RS04190 at 797.536 kb on + strand, within MMP_RS04190 at 797.597 kb on + strand, within MMP_RS04190 at 797.709 kb on + strand, within MMP_RS04190 at 797.723 kb on - strand, within MMP_RS04190 at 797.728 kb on - strand, within MMP_RS04190 at 797.728 kb on - strand, within MMP_RS04190 at 797.810 kb on + strand at 797.810 kb on + strand at 797.818 kb on - strand at 797.821 kb on + strand at 797.821 kb on + strand at 797.859 kb on - strand at 797.959 kb on + strand at 797.959 kb on + strand at 798.002 kb on + strand at 798.090 kb on + strand at 798.090 kb on + strand at 798.179 kb on + strand, within MMP_RS04195 at 798.301 kb on - strand, within MMP_RS04195 at 798.508 kb on - strand, within MMP_RS04195 at 798.511 kb on - strand, within MMP_RS04195 at 798.574 kb on - strand at 798.578 kb on - strand at 798.579 kb on + strand at 798.608 kb on + strand at 798.612 kb on + strand at 798.622 kb on - strand at 798.623 kb on + strand at 798.639 kb on + strand at 798.687 kb on + strand at 798.687 kb on + strand at 798.743 kb on + strand, within MMP_RS04200 at 798.759 kb on + strand, within MMP_RS04200 at 798.782 kb on - strand, within MMP_RS04200 at 798.806 kb on - strand, within MMP_RS04200 at 798.807 kb on + strand, within MMP_RS04200 at 798.837 kb on - strand, within MMP_RS04200 at 798.858 kb on + strand, within MMP_RS04200 at 798.886 kb on - strand, within MMP_RS04200 at 799.090 kb on - strand, within MMP_RS04200 at 799.215 kb on + strand, within MMP_RS04200 at 799.219 kb on - strand, within MMP_RS04200 at 799.273 kb on - strand, within MMP_RS04200 at 799.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Chloride 250 mM remove 797,099 - MMP_RS04185 0.87 -0.9 797,100 + MMP_RS04185 0.87 +0.6 797,172 - -5.2 797,273 - +2.0 797,305 + +0.0 797,361 - +0.3 797,362 + -0.1 797,362 + -0.0 797,362 + -0.3 797,365 + +1.1 797,403 + MMP_RS04190 0.15 +0.3 797,536 + MMP_RS04190 0.41 +0.2 797,597 + MMP_RS04190 0.52 +1.3 797,709 + MMP_RS04190 0.74 +0.8 797,723 - MMP_RS04190 0.77 +4.1 797,728 - MMP_RS04190 0.78 +0.8 797,728 - MMP_RS04190 0.78 +1.7 797,810 + +2.7 797,810 + -0.6 797,818 - +1.2 797,821 + -1.0 797,821 + -0.1 797,859 - +0.1 797,959 + -2.3 797,959 + +0.6 798,002 + +0.5 798,090 + +0.8 798,090 + -0.5 798,179 + MMP_RS04195 0.22 -0.1 798,301 - MMP_RS04195 0.45 +0.9 798,508 - MMP_RS04195 0.83 -0.4 798,511 - MMP_RS04195 0.84 -2.2 798,574 - +0.7 798,578 - -0.3 798,579 + -0.4 798,608 + -0.0 798,612 + +0.3 798,622 - +0.3 798,623 + -0.2 798,639 + +0.5 798,687 + -0.5 798,687 + -0.0 798,743 + MMP_RS04200 0.16 -0.5 798,759 + MMP_RS04200 0.18 -0.9 798,782 - MMP_RS04200 0.22 +1.3 798,806 - MMP_RS04200 0.25 -0.3 798,807 + MMP_RS04200 0.25 +1.1 798,837 - MMP_RS04200 0.29 +0.3 798,858 + MMP_RS04200 0.32 -0.7 798,886 - MMP_RS04200 0.36 -5.4 799,090 - MMP_RS04200 0.64 +0.4 799,215 + MMP_RS04200 0.82 -0.7 799,219 - MMP_RS04200 0.82 +0.6 799,273 - MMP_RS04200 0.90 +0.1 799,470 - -1.9
Or see this region's nucleotide sequence