Experiment: McCas_H2_complete 1,2-dimethyl-5-nitroimidazole 0.00833333333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02515 and MMP_RS02520 are separated by 26 nucleotides MMP_RS02520 and MMP_RS02525 are separated by 134 nucleotides MMP_RS02525 and MMP_RS02530 are separated by 732 nucleotides
MMP_RS02515: MMP_RS02515 - hypothetical protein, at 469,177 to 470,175
_RS02515
MMP_RS02520: MMP_RS02520 - hypothetical protein, at 470,202 to 471,008
_RS02520
MMP_RS02525: MMP_RS02525 - DUF2254 domain-containing protein, at 471,143 to 472,984
_RS02525
MMP_RS02530: MMP_RS02530 - type II toxin-antitoxin system mRNA interferase toxin RelE/StbE family, at 473,717 to 474,019
_RS02530
Position (kb) 471
472
473 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 470.200 kb on - strand at 470.212 kb on - strand at 470.216 kb on - strand at 470.267 kb on - strand at 470.270 kb on - strand at 470.277 kb on - strand at 470.277 kb on - strand at 470.278 kb on + strand at 470.278 kb on + strand at 470.298 kb on - strand, within MMP_RS02520 at 470.299 kb on + strand, within MMP_RS02520 at 470.299 kb on + strand, within MMP_RS02520 at 470.299 kb on + strand, within MMP_RS02520 at 470.299 kb on + strand, within MMP_RS02520 at 470.299 kb on + strand, within MMP_RS02520 at 470.304 kb on + strand, within MMP_RS02520 at 470.304 kb on + strand, within MMP_RS02520 at 470.308 kb on + strand, within MMP_RS02520 at 470.363 kb on + strand, within MMP_RS02520 at 470.377 kb on - strand, within MMP_RS02520 at 470.377 kb on - strand, within MMP_RS02520 at 470.377 kb on - strand, within MMP_RS02520 at 470.377 kb on - strand, within MMP_RS02520 at 470.428 kb on - strand, within MMP_RS02520 at 470.441 kb on - strand, within MMP_RS02520 at 470.441 kb on - strand, within MMP_RS02520 at 470.447 kb on - strand, within MMP_RS02520 at 470.450 kb on + strand, within MMP_RS02520 at 470.474 kb on + strand, within MMP_RS02520 at 470.487 kb on + strand, within MMP_RS02520 at 470.499 kb on + strand, within MMP_RS02520 at 470.576 kb on + strand, within MMP_RS02520 at 470.582 kb on - strand, within MMP_RS02520 at 470.582 kb on - strand, within MMP_RS02520 at 470.587 kb on - strand, within MMP_RS02520 at 470.655 kb on + strand, within MMP_RS02520 at 470.662 kb on - strand, within MMP_RS02520 at 470.692 kb on - strand, within MMP_RS02520 at 470.764 kb on - strand, within MMP_RS02520 at 470.765 kb on + strand, within MMP_RS02520 at 470.913 kb on + strand, within MMP_RS02520 at 470.913 kb on + strand, within MMP_RS02520 at 470.965 kb on - strand at 470.965 kb on - strand at 470.971 kb on - strand at 470.971 kb on - strand at 470.971 kb on - strand at 470.998 kb on - strand at 470.998 kb on - strand at 470.999 kb on + strand at 470.999 kb on + strand at 471.050 kb on - strand at 471.120 kb on + strand at 471.121 kb on - strand at 471.314 kb on - strand at 471.314 kb on - strand at 471.315 kb on + strand at 471.315 kb on + strand at 471.315 kb on + strand at 471.316 kb on - strand at 471.413 kb on + strand, within MMP_RS02525 at 471.502 kb on - strand, within MMP_RS02525 at 471.502 kb on - strand, within MMP_RS02525 at 471.527 kb on + strand, within MMP_RS02525 at 471.537 kb on - strand, within MMP_RS02525 at 471.600 kb on - strand, within MMP_RS02525 at 471.693 kb on + strand, within MMP_RS02525 at 471.751 kb on + strand, within MMP_RS02525 at 471.776 kb on + strand, within MMP_RS02525 at 471.779 kb on + strand, within MMP_RS02525 at 471.780 kb on - strand, within MMP_RS02525 at 471.781 kb on + strand, within MMP_RS02525 at 471.817 kb on - strand, within MMP_RS02525 at 471.840 kb on - strand, within MMP_RS02525 at 471.885 kb on - strand, within MMP_RS02525 at 471.885 kb on - strand, within MMP_RS02525 at 471.887 kb on - strand, within MMP_RS02525 at 471.904 kb on - strand, within MMP_RS02525 at 471.905 kb on + strand, within MMP_RS02525 at 471.907 kb on - strand, within MMP_RS02525 at 471.908 kb on + strand, within MMP_RS02525 at 471.915 kb on + strand, within MMP_RS02525 at 471.919 kb on - strand, within MMP_RS02525 at 471.919 kb on - strand, within MMP_RS02525 at 471.922 kb on + strand, within MMP_RS02525 at 471.926 kb on - strand, within MMP_RS02525 at 471.927 kb on + strand, within MMP_RS02525 at 471.931 kb on + strand, within MMP_RS02525 at 471.990 kb on + strand, within MMP_RS02525 at 471.990 kb on + strand, within MMP_RS02525 at 471.990 kb on + strand, within MMP_RS02525 at 472.013 kb on + strand, within MMP_RS02525 at 472.013 kb on + strand, within MMP_RS02525 at 472.013 kb on + strand, within MMP_RS02525 at 472.015 kb on + strand, within MMP_RS02525 at 472.046 kb on - strand, within MMP_RS02525 at 472.070 kb on + strand, within MMP_RS02525 at 472.130 kb on + strand, within MMP_RS02525 at 472.133 kb on - strand, within MMP_RS02525 at 472.133 kb on - strand, within MMP_RS02525 at 472.136 kb on - strand, within MMP_RS02525 at 472.153 kb on - strand, within MMP_RS02525 at 472.158 kb on - strand, within MMP_RS02525 at 472.161 kb on + strand, within MMP_RS02525 at 472.177 kb on - strand, within MMP_RS02525 at 472.186 kb on - strand, within MMP_RS02525 at 472.453 kb on - strand, within MMP_RS02525 at 472.456 kb on - strand, within MMP_RS02525 at 472.517 kb on - strand, within MMP_RS02525 at 472.580 kb on - strand, within MMP_RS02525 at 472.654 kb on - strand, within MMP_RS02525 at 472.655 kb on + strand, within MMP_RS02525 at 472.656 kb on - strand, within MMP_RS02525 at 472.656 kb on - strand, within MMP_RS02525 at 472.657 kb on + strand, within MMP_RS02525 at 472.659 kb on + strand, within MMP_RS02525 at 472.659 kb on + strand, within MMP_RS02525 at 472.659 kb on + strand, within MMP_RS02525 at 472.659 kb on + strand, within MMP_RS02525 at 472.661 kb on - strand, within MMP_RS02525 at 472.773 kb on + strand, within MMP_RS02525 at 472.831 kb on + strand at 472.837 kb on - strand at 472.862 kb on + strand at 472.862 kb on + strand at 472.862 kb on + strand at 472.911 kb on - strand at 472.911 kb on - strand at 472.965 kb on + strand at 473.003 kb on + strand at 473.008 kb on - strand at 473.095 kb on - strand at 473.167 kb on - strand at 473.167 kb on - strand at 473.168 kb on + strand at 473.213 kb on + strand at 473.379 kb on - strand at 473.445 kb on + strand at 473.450 kb on - strand at 473.460 kb on - strand at 473.650 kb on + strand at 473.754 kb on - strand, within MMP_RS02530 at 473.755 kb on + strand, within MMP_RS02530 at 473.776 kb on + strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.788 kb on + strand, within MMP_RS02530 at 473.789 kb on - strand, within MMP_RS02530 at 473.838 kb on - strand, within MMP_RS02530 at 473.948 kb on + strand, within MMP_RS02530
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete 1,2-dimethyl-5-nitroimidazole 0.00833333333333333 mM remove 470,200 - +0.5 470,212 - -0.4 470,216 - +0.3 470,267 - +0.1 470,270 - -0.3 470,277 - +3.2 470,277 - +0.4 470,278 + -0.1 470,278 + +2.2 470,298 - MMP_RS02520 0.12 +1.8 470,299 + MMP_RS02520 0.12 -0.2 470,299 + MMP_RS02520 0.12 +0.3 470,299 + MMP_RS02520 0.12 -0.1 470,299 + MMP_RS02520 0.12 +2.7 470,299 + MMP_RS02520 0.12 +2.0 470,304 + MMP_RS02520 0.13 -1.0 470,304 + MMP_RS02520 0.13 +1.2 470,308 + MMP_RS02520 0.13 +1.0 470,363 + MMP_RS02520 0.20 -3.0 470,377 - MMP_RS02520 0.22 -0.3 470,377 - MMP_RS02520 0.22 +0.8 470,377 - MMP_RS02520 0.22 -0.4 470,377 - MMP_RS02520 0.22 +0.9 470,428 - MMP_RS02520 0.28 +0.2 470,441 - MMP_RS02520 0.30 -0.9 470,441 - MMP_RS02520 0.30 +0.6 470,447 - MMP_RS02520 0.30 +0.5 470,450 + MMP_RS02520 0.31 -0.7 470,474 + MMP_RS02520 0.34 -0.7 470,487 + MMP_RS02520 0.35 +0.4 470,499 + MMP_RS02520 0.37 -0.1 470,576 + MMP_RS02520 0.46 -0.3 470,582 - MMP_RS02520 0.47 -1.8 470,582 - MMP_RS02520 0.47 -1.3 470,587 - MMP_RS02520 0.48 -0.1 470,655 + MMP_RS02520 0.56 +0.9 470,662 - MMP_RS02520 0.57 +0.4 470,692 - MMP_RS02520 0.61 +3.4 470,764 - MMP_RS02520 0.70 -0.3 470,765 + MMP_RS02520 0.70 +2.3 470,913 + MMP_RS02520 0.88 +1.9 470,913 + MMP_RS02520 0.88 +1.5 470,965 - +0.0 470,965 - +0.7 470,971 - +0.6 470,971 - +0.8 470,971 - -2.6 470,998 - -2.2 470,998 - -2.5 470,999 + +0.2 470,999 + +0.5 471,050 - -0.9 471,120 + +0.8 471,121 - +0.4 471,314 - +3.7 471,314 - -0.7 471,315 + -0.5 471,315 + +0.1 471,315 + -0.3 471,316 - +0.4 471,413 + MMP_RS02525 0.15 -1.4 471,502 - MMP_RS02525 0.19 +0.4 471,502 - MMP_RS02525 0.19 +2.3 471,527 + MMP_RS02525 0.21 +0.6 471,537 - MMP_RS02525 0.21 -0.5 471,600 - MMP_RS02525 0.25 -3.0 471,693 + MMP_RS02525 0.30 -0.6 471,751 + MMP_RS02525 0.33 +0.5 471,776 + MMP_RS02525 0.34 +1.2 471,779 + MMP_RS02525 0.35 +0.0 471,780 - MMP_RS02525 0.35 -0.0 471,781 + MMP_RS02525 0.35 +2.3 471,817 - MMP_RS02525 0.37 +1.4 471,840 - MMP_RS02525 0.38 +2.3 471,885 - MMP_RS02525 0.40 -0.1 471,885 - MMP_RS02525 0.40 +0.6 471,887 - MMP_RS02525 0.40 -0.5 471,904 - MMP_RS02525 0.41 +0.2 471,905 + MMP_RS02525 0.41 +3.1 471,907 - MMP_RS02525 0.41 -0.7 471,908 + MMP_RS02525 0.42 -1.2 471,915 + MMP_RS02525 0.42 -0.8 471,919 - MMP_RS02525 0.42 -2.7 471,919 - MMP_RS02525 0.42 +1.0 471,922 + MMP_RS02525 0.42 -0.5 471,926 - MMP_RS02525 0.43 +2.1 471,927 + MMP_RS02525 0.43 -1.0 471,931 + MMP_RS02525 0.43 -1.5 471,990 + MMP_RS02525 0.46 -0.6 471,990 + MMP_RS02525 0.46 +2.4 471,990 + MMP_RS02525 0.46 -0.7 472,013 + MMP_RS02525 0.47 -1.1 472,013 + MMP_RS02525 0.47 +0.5 472,013 + MMP_RS02525 0.47 -0.1 472,015 + MMP_RS02525 0.47 +1.1 472,046 - MMP_RS02525 0.49 +0.5 472,070 + MMP_RS02525 0.50 -0.6 472,130 + MMP_RS02525 0.54 -3.1 472,133 - MMP_RS02525 0.54 -0.4 472,133 - MMP_RS02525 0.54 +1.4 472,136 - MMP_RS02525 0.54 +1.0 472,153 - MMP_RS02525 0.55 +0.1 472,158 - MMP_RS02525 0.55 +0.6 472,161 + MMP_RS02525 0.55 -2.0 472,177 - MMP_RS02525 0.56 +0.7 472,186 - MMP_RS02525 0.57 +3.1 472,453 - MMP_RS02525 0.71 +1.0 472,456 - MMP_RS02525 0.71 +1.2 472,517 - MMP_RS02525 0.75 -1.7 472,580 - MMP_RS02525 0.78 +3.2 472,654 - MMP_RS02525 0.82 +0.3 472,655 + MMP_RS02525 0.82 +2.7 472,656 - MMP_RS02525 0.82 +3.9 472,656 - MMP_RS02525 0.82 +1.0 472,657 + MMP_RS02525 0.82 -2.1 472,659 + MMP_RS02525 0.82 +2.2 472,659 + MMP_RS02525 0.82 -0.5 472,659 + MMP_RS02525 0.82 +0.1 472,659 + MMP_RS02525 0.82 +0.6 472,661 - MMP_RS02525 0.82 +0.9 472,773 + MMP_RS02525 0.88 +2.9 472,831 + -0.6 472,837 - -2.0 472,862 + -1.5 472,862 + -0.9 472,862 + +0.4 472,911 - +1.4 472,911 - +0.3 472,965 + -0.9 473,003 + +0.0 473,008 - +1.1 473,095 - -0.9 473,167 - +0.9 473,167 - -1.2 473,168 + +1.0 473,213 + +1.0 473,379 - +0.7 473,445 + -0.8 473,450 - -0.6 473,460 - +0.5 473,650 + -0.1 473,754 - MMP_RS02530 0.12 +2.1 473,755 + MMP_RS02530 0.13 -1.9 473,776 + MMP_RS02530 0.19 +4.6 473,787 - MMP_RS02530 0.23 +1.4 473,787 - MMP_RS02530 0.23 -2.5 473,787 - MMP_RS02530 0.23 +3.0 473,788 + MMP_RS02530 0.23 +0.1 473,789 - MMP_RS02530 0.24 -2.2 473,838 - MMP_RS02530 0.40 -0.0 473,948 + MMP_RS02530 0.76 -0.0
Or see this region's nucleotide sequence