Experiment: McCas_H2_complete Magnesium chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00735 and MMP_RS00740 are separated by 306 nucleotides MMP_RS00740 and MMP_RS00745 are separated by 77 nucleotides MMP_RS00745 and MMP_RS00750 are separated by 15 nucleotides
MMP_RS00735: MMP_RS00735 - 5 10-methenyltetrahydromethanopterin hydrogenase, at 133,625 to 134,689
_RS00735
MMP_RS00740: MMP_RS00740 - cyclase family protein, at 134,996 to 135,565
_RS00740
MMP_RS00745: MMP_RS00745 - histidinol phosphate phosphatase domain-containing protein, at 135,643 to 136,299
_RS00745
MMP_RS00750: MMP_RS00750 - fumarate hydratase, at 136,315 to 137,160
_RS00750
Position (kb) 134
135
136 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 134.255 kb on - strand, within MMP_RS00735 at 134.255 kb on - strand, within MMP_RS00735 at 134.424 kb on + strand, within MMP_RS00735 at 134.713 kb on - strand at 134.713 kb on - strand at 134.723 kb on - strand at 134.757 kb on + strand at 134.760 kb on - strand at 134.760 kb on - strand at 134.782 kb on + strand at 134.863 kb on - strand at 134.900 kb on + strand at 134.982 kb on + strand at 135.287 kb on - strand, within MMP_RS00740 at 135.338 kb on + strand, within MMP_RS00740 at 135.421 kb on - strand, within MMP_RS00740 at 135.422 kb on + strand, within MMP_RS00740 at 135.565 kb on - strand at 135.600 kb on + strand at 135.606 kb on + strand at 135.613 kb on - strand at 135.613 kb on - strand at 135.619 kb on + strand at 135.675 kb on - strand at 135.800 kb on - strand at 136.395 kb on + strand at 136.454 kb on - strand, within MMP_RS00750
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Magnesium chloride 250 mM remove 134,255 - MMP_RS00735 0.59 -1.7 134,255 - MMP_RS00735 0.59 -0.8 134,424 + MMP_RS00735 0.75 -0.8 134,713 - -2.8 134,713 - +0.4 134,723 - +0.5 134,757 + -0.7 134,760 - -1.2 134,760 - -2.4 134,782 + -0.6 134,863 - -0.2 134,900 + -0.4 134,982 + +1.1 135,287 - MMP_RS00740 0.51 +0.4 135,338 + MMP_RS00740 0.60 -0.2 135,421 - MMP_RS00740 0.75 -1.6 135,422 + MMP_RS00740 0.75 -0.8 135,565 - -0.3 135,600 + +0.0 135,606 + -0.4 135,613 - -2.1 135,613 - +0.1 135,619 + -1.2 135,675 - -0.2 135,800 - -2.4 136,395 + -0.6 136,454 - MMP_RS00750 0.16 -2.6
Or see this region's nucleotide sequence