Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06885

Experiment: McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06880 and MMP_RS06885 are separated by 382 nucleotidesMMP_RS06885 and MMP_RS06890 are separated by 19 nucleotidesMMP_RS06890 and MMP_RS06895 are separated by 10 nucleotides MMP_RS06880: MMP_RS06880 - chorismate synthase, at 1,313,078 to 1,314,199 _RS06880 MMP_RS06885: MMP_RS06885 - 50S ribosome-binding GTPase, at 1,314,582 to 1,315,736 _RS06885 MMP_RS06890: MMP_RS06890 - mevalonate kinase, at 1,315,756 to 1,316,709 _RS06890 MMP_RS06895: MMP_RS06895 - selenocysteine-specific translation elongation factor, at 1,316,720 to 1,318,126 _RS06895 Position (kb) 1314 1315 1316Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1314.178 kb on - strandat 1314.197 kb on - strandat 1314.198 kb on + strandat 1314.286 kb on - strandat 1314.287 kb on + strandat 1314.313 kb on + strandat 1314.321 kb on - strandat 1314.321 kb on - strandat 1314.321 kb on - strandat 1314.373 kb on + strandat 1314.448 kb on + strandat 1314.504 kb on + strandat 1314.504 kb on + strandat 1314.553 kb on + strandat 1314.559 kb on - strandat 1314.560 kb on + strandat 1314.564 kb on - strandat 1314.566 kb on - strandat 1314.566 kb on - strandat 1314.567 kb on + strandat 1314.567 kb on + strandat 1314.569 kb on + strandat 1314.569 kb on + strandat 1314.569 kb on + strandat 1314.569 kb on + strandat 1314.590 kb on - strandat 1314.741 kb on - strand, within MMP_RS06885at 1314.771 kb on - strand, within MMP_RS06885at 1314.926 kb on + strand, within MMP_RS06885at 1315.212 kb on + strand, within MMP_RS06885at 1315.280 kb on + strand, within MMP_RS06885at 1316.083 kb on - strandat 1316.104 kb on + strandat 1316.147 kb on + strandat 1316.274 kb on + strandat 1316.539 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM
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1,314,178 - -2.5
1,314,197 - +0.9
1,314,198 + -0.6
1,314,286 - +0.8
1,314,287 + +1.0
1,314,313 + -3.6
1,314,321 - -0.2
1,314,321 - +1.2
1,314,321 - -3.9
1,314,373 + -0.3
1,314,448 + +1.3
1,314,504 + +4.6
1,314,504 + -0.1
1,314,553 + +0.7
1,314,559 - -0.1
1,314,560 + +1.8
1,314,564 - +0.4
1,314,566 - +1.1
1,314,566 - +1.7
1,314,567 + +1.4
1,314,567 + +1.8
1,314,569 + -0.2
1,314,569 + +0.4
1,314,569 + +1.6
1,314,569 + -0.2
1,314,590 - -0.5
1,314,741 - MMP_RS06885 0.14 -4.5
1,314,771 - MMP_RS06885 0.16 -4.7
1,314,926 + MMP_RS06885 0.30 -4.1
1,315,212 + MMP_RS06885 0.55 -2.1
1,315,280 + MMP_RS06885 0.60 -2.2
1,316,083 - -3.2
1,316,104 + -3.7
1,316,147 + -2.6
1,316,274 + +1.4
1,316,539 - -3.4

Or see this region's nucleotide sequence