Strain Fitness in Methanococcus maripaludis S2 around MMP_RS01395

Experiment: McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS01385 and MMP_RS01390 are separated by 11 nucleotidesMMP_RS01390 and MMP_RS01395 are separated by 131 nucleotidesMMP_RS01395 and MMP_RS01400 are separated by 87 nucleotides MMP_RS01385: MMP_RS01385 - imidazole glycerol phosphate synthase subunit HisH, at 258,316 to 258,927 _RS01385 MMP_RS01390: MMP_RS01390 - TATA-box-binding protein, at 258,939 to 259,484 _RS01390 MMP_RS01395: MMP_RS01395 - 50S ribosomal protein P1, at 259,616 to 259,915 _RS01395 MMP_RS01400: MMP_RS01400 - 50S ribosomal protein L10, at 260,003 to 261,010 _RS01400 Position (kb) 259 260Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 258.865 kb on - strand, within MMP_RS01385at 258.908 kb on - strandat 258.943 kb on - strandat 258.974 kb on - strandat 258.993 kb on - strandat 258.997 kb on - strandat 258.998 kb on + strandat 258.998 kb on + strandat 259.147 kb on + strandat 259.525 kb on + strandat 259.903 kb on + strandat 259.936 kb on + strandat 259.992 kb on - strandat 259.995 kb on + strandat 260.284 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM
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258,865 - MMP_RS01385 0.90 -4.0
258,908 - -1.3
258,943 - -2.8
258,974 - +0.3
258,993 - -4.2
258,997 - -2.3
258,998 + -2.9
258,998 + +0.0
259,147 + +0.1
259,525 + -2.0
259,903 + -0.8
259,936 + -3.0
259,992 - -3.0
259,995 + -3.5
260,284 - -2.5

Or see this region's nucleotide sequence